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pcDNA3.1-pA83-HA-TAZ (#RDB12193)

Expression clone of mouse TAZ

Clone info. Expression clone of mouse TAZ. cDNA fragment 281 to 1,468 of NM_133784.3 (CDS, 281..1468) is cloned.
Comment See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Two instead of one T7 promoter sequences are appeared.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Wwtr1|TAZ
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12193 pcDNA3.1-pA83-HA-TAZ DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-HA-TAZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12193).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12193_A3Iap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro x2,insert 5'(HA,mTAZ 5')
Sequence file: RDB12193_A3Iae.seq check
>12193_2_CMV_F_A09_03.ab1
    1 NNNNNNNNNN NNNNNTTGNN GCNNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCACTA
  181 GTCCAGTGTG GTGGAATTGT AATACGACTC ACTATAGGGA GACCCAAGCT TGCCGCCACC
  241 ATGTACCCAT ACGACGTTCC AGATTACGCT GGTACCATGA ATCCGTCCTC GGTGCCCCAT
  301 CCGCTCCCGC CGCCAGGGCA GCAAGTCATC CACGTCACGC AGGACCTGGA CACCGACCTC
  361 GAAGCCCTCT TCAACTCTGT CATGAACCCC AAGCCCAGCT CATGGCGGAA AAAGATCCTC
  421 CCGGAGTCCT TCTTTAAGGA GCCCGATTCC GGCTCGCACT CGCGCCAATC CAGCACAGAC
  481 TCATCAGGCG GCCACCCGGG GCCTCGACTA GCTGGCGGCG CGCAGCACGT CCGCTCGCAC
  541 TCGTCGCCCG CATCCCTGCA GCTGGGCACC GGTGCGGGAG CCGCTGGAGG CCCTGCACAN
601 CANNNNNNNN NN
//
Primer: GNP Primer #10 (Pr0763)
Region: TRF2 3’-UTR and polyA,insert 3' (mTAZ 3')
Sequence file: RDB12193_A3Iaf.seq check
>12193_2_GNP10_B09_06.ab1
    1 NNNNNNNNNN NNNNTNATNN NNNNNANAAC GTTTACNGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCGCCA CTGTGCTGGA TATCTGCAGA ATTTAGAAGG CACAGTCGAG GCTGATCAGC
  121 GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGCGGC
  181 CGCCAGTGTG ATGGATATCT GCAGAATTCT TACAGCCAGG TTAGAAAGGG CTCGCTTTTG
  241 TTCAGAGCAG ACTCTACATC ATTGAAGAGA GGGATCAGAT CTTCAGACTC CAAAGTCCCG
  301 AGGTCAACAT TTGTTCCTGG AAGGCAGTCC AGGAAATCAG GGAAGCGGGT CTGCTGGGGA
  361 TTGACGGTCA TGGGTGTCTG ACCGGAATTT TCACCTGTAT CCATCTCGTC CATGTTGCTG
  421 AGGAAGTCTT CTGGAGTTGT GGGGACACTG TAGCACCCTA ACCCCAGGCC ACTGTCTGTG
  481 CTCTGCTCCC GTGAATGATA GGGCCCTCCA TTGAGGAAAG GATCTGAGCT ACTGTTGGTG
  541 ACAGATCTCA TATCTGTGCT CATGGCAGGC GTGTTGACAG GGGCCATGGT CTCGGTTTCC
  601 ATGGGGAGCT GTCGGCAGAG GGCAGCTTCC TGCCTCATGA GCTCCTCTTG ACGCATCCTA
  661 ATCCTCTCTC TCTCCATCTG GATCCTCTGA AGCCGCAGTT TCTGCTGCTG CTGCTGCTGA
  721 GTGGTCAGTG CATTGGGCAC ACTCATGAGC CCTGTGGGTT GNTTCTGANT CGGGTGGTTC
  781 TGTGGACTCA GGCTAGTGNC CACGACTTGC TGGTGTTGGT GANTCATTGC GAGATTCGGC
  841 TGGGANACTG CCNTGGACCT CTGTGGCACC NANGTGGAAN TGANGGANGG GTGGANGTTC
  901 ACATGATTCA NANGNTGANN CNTCACCTTN CTNGGGNCTT GNCNNGTGGN GATTTTCNCT
961 ANNNGATNNA NNNNAGTATC TCTGGNCNNN GNNNNNNNNN N
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,TRF2 3’-UTR and polyA
Sequence file: RDB12193_A3Iag.seq check
>12193_12193_B2KlF_bGH_rev3_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNGNNNNG GNNNNACNAC NGNNGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGGGTTTAAA CTCAATGGTG ATGGTGATGA TGACCGGTAT GCATATTCAG
  121 ATCCTCTTCT GAGATGAGTT TTTGTTCGAA GGGCCCTCTA GACTCGAGGT ACGCCACAGC
  181 GTATGATGCG TATTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
241 TTTTTTTTTT TTTTTTTTTN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori,NeoR
Sequence file: RDB12193_A3Iah.seq check
>12193_12193_B2KlF_SV40pro_ori_F_F01_06_ABI08.ab1
    1 NNNNNNNCNN NNNNNNNTCT NNNNNNNTCN NNAAGTAGTG AGGAGGCTTT TTTGGAGGCC
   61 TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA
  121 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
  181 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
  241 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
  301 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG
  361 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
  421 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT
  481 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
  541 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG
  601 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
  661 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
  721 CGAATATCAT GGTGGAAAAT GGNCGCTTTT CTGGATTCNT CGACTGTGGC CGGCTGGGTG
  781 TGGCGGACCG CTATCNNGAC ATAGCGTTGG CTANNCGTGA TATTGCTGAA GANCTTNNNN
  841 GCGAATGGGC TGANCGCTTC CTCNNGCTTT ACGGTATCGC CGCTCCCNAN TCNCANNGCA
  901 TCNCCNTCTA TCGCCTTCNT GACNANTTCN TCTGANCGGG ANNCNNGGGN TCGCGAANNG
  961 ANNNACCNAG CGNNGCCNNN NNNGNCNTCN CNANNNTNNN NNNCNNNNNN NCNNTNNNNG
1021 AANNNNNGGN CTNNNNNNNN TTTNNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]