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HA-Amot130-m5/pcDNA3.1-pA83 (#RDB12206)

Expression clone of mouse Amot (p130), mPY123 mutation

Alternative name HA-Amot-mPY123
Clone info. Expression clone of mouse Amot (p130), three mutations in PY motifs (mPY123). HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12206 HA-Amot130-m5/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130-m5/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12206).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12206_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12206_A5Hpa.seq check
>12206_12206_A5Hp_CMV-Forward_G11_20.ab1
    1 GCTATCCACT TCCGCCCCCC ATTGAAGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTAGCTTGC CGCCACCATG
  181 TACCCATACG ACGTTCCAGA TTACGCTGGA TCCAGAAGTT CTGACGATCA GCCAAGTGGA
  241 GGAACGACGG TACTGCAACG GCTGCTTCAA GAGCAGCTTC GCTATGGCAA TCCCAGTGAG
  301 AATCGCAGTC TGCTTGCCAT ACACCAGCAA GCCACAGGGA ATAGCTCTCC ATTTTCCACG
  361 GGCAGTGGGA ACCAAGGCCC TCAGAATGAT GTATTGAGTT CCCAGGATCA CCACCAGCAG
  421 CAGCTTGTGG CACACCCTGC TCGCCAAGAG CCCCAGGGTC AGGAAATCCA GTCAGAAAAC
  481 GGCGTCATGG AGAAGCAGCT GTCCCCTCGA ATGCAGAATA ACGAAGAATT CGCAACCCAT
  541 GAAGAGGCCA AGGTCCAGTC CCAGTACTTC AGGGGCCAGC AGCATGCTAG TGTTGGGGCT
  601 GCTTTCTATG TCACAGGAGT CACCAACCAG AAGATGAGAA CCGAGGGACG CCCGTCAGTC
  661 CAGCGCCTCA CGCCCGGGAA GATGCACCAA GATGAAGGCC TTCGAGATCT TAAGCAAGGG
  721 CATGTCCGAT CCTTGAGTGA AAGACTGATG CAGATGTCAT TGGCTACTAG TGGAGTTAAA
  781 GCCCACCCAC CTGTTACCAG TGCTCCTCTC TCCCCACCCA CAACCCAATG ATCTCTACAA
  841 GAATGCCACC AGTTCCAGTG AATTCTACAA GGCCCAAAGG GCCACCTCCA AGCCAACATA
  901 GTCTGAAGGG CATGGAAACA CCGAGGTCCC GGATCCGAAC ACCCTTTCAA GGGCGTGCCA
  961 TCCCAGTCTG TAGTGTGTAA GTCCAAAGAG CCAGGGCACT TCTATAGTGA GCATCGCCTG
 1021 AACCCAGCTA GGAGAACAGA GGGGGCAACT GATGAGTATC AGCCACGGGT ACCGAGCATG
 1081 GGGCAGCCAA GGCAACACAG GGACATTCCT CCTTCGCTTG TCTTTTGGTC AGTCAGGAAA
1141 CTTCTTCAAC CCTTCCATAT GAGACCTTAA CCATTCCTTT CCCCTTGACA
//
Primer: mAmot-M#2
Sequence file: RDB12206_A5Hpb.seq check
>12206_12206_A5Hp_mAmot-M#2_C09_09.ab1
    1 TGGGTTATTG CAGACTGCTG AGCCGAGGCA TAGCTGAGTA GGCTTCTCCT GGCTGCTGCT
   61 GCGCTGAAGT CAGGCTGGCC TGGGCGTGAC GGTAGTGGTC CCCCTGATGA CTCTGACTCG
  121 AAAGAAGATG CTGCTGATGC CTTGACAGGT GAGCAGAGTG GTCACCTTGG TTCGGGACCA
  181 GGTGTTGACC AGGAGGCAAT CTAGTGGACG GCGGAGACTG CAGCAAGGGC AAGCTGGAAG
  241 CCCCAGCTGT CAGGGAAGAA GTAGGGCTAT GAGGTTGAGA GTTCCTGGCT GACAAAGACA
  301 GCGAGGAGAT GTCCTGTGTT GCCCTGGCTG CCCCATGCTC GGTACCGTGC TGATACCTCA
  361 TCAGTTGCCC CTCTGTTCTC CCTGGCTGGT TCAGGCGATG CTCACTATAG AAGTGCCCTG
  421 GCTCTTGGGA CTTACACACT ACAGACTGGG ATGGCACGCC CTTGAAAGGG TGTTCGGATC
  481 CGGGACCTCG GTGTTCCATG CCCTTCAGAC TATGTTGGCT TGGAGGTGGC CCTTGGGCCT
  541 TGTAGAATTC ACTGGAACTG GTGGCATTCT TGTAGAGATC ATTGGGTTGT GGTGGGGAGA
  601 GAGGAGCACT GGTAACAGGT GGGTGGGCTT TAACTCCACT AGTAGCCAAT GACATCTGCA
  661 TCAGTCTTTC ACTCAAGGAT CGGACATGCC CTTGCTTAAG ATCTCGAAGG CCTTCATCTT
  721 GGTGCATCTT CCCGGGCGTG AGGCGCTGGA CTGACGGGCG TCCCTCGGTT CTCATCTTCT
  781 GGTTGGTGAC TCCTGTGACA TAGAAAGCAG CCCCAACACT AGCATGCTGC TGGCCCCTGA
  841 AGTACTGGGA CTGGACCTTG GCCTCTTCAT GGGTTGCGAA TTCTTCGTTA TTCTGCATTC
  901 GAGGGGACAG CTGCTTCTCC ATGACGCCGT TTTCTGACTG GATTTCCTGA CCCTGGGGCT
  961 CTTGGCGAGC AGGTGTGCCA CAAGCTGCTG CTGGTGTGAT CCTGGAACTC AATACATCAT
 1021 TCTGAGGGCC TTGATCCCAC TGCCCGTGAA AATGGAGAGC TATCCTGTGC TTGCTGTGTA
 1081 TGGCAGCCAG ACTGCCAATC CTCACTGGGA TGCATAGCGA AGCTGCTCTG GAGCCAGTCG
1141 GTTGCAGTAC GTCGATTCTC CTGCTGATCG ATCGCACTTT CTGTGAAATT CGA
//
Primer: GNP_primer#10
Sequence file: RDB12206_A5Hpc.seq check
>12206_12206_A5Hp_GNP_primer#10_F05_17.ab1
    1 TTTTTTGTGC CATTCATTTA AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
  121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
  181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
  241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
  301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
  361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
  421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
  481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
  541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
  601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
  661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
  721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
  781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
  841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
  901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGCGGCAGCA GCAGCTGGAG
  961 CATCAACAGC AACTTGTAGC AGCAGCTACC ATGATAGTGG TGTTTGTGGC ACCATGCCAT
1021 GTTGGTGCAC CAGTTGCATG ATGGCAACCA CCAGGTGCGC AAGCAC
//
Primer: BGH_rev
Sequence file: RDB12206_A5Hpd.seq check
>12206_12206_A5Hp_BGH_rev_H08_23.ab1
    1 CATACTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTCCCT TTCCCAAATT TTTTTTTGGG CCCTTTCATT TAAAAAAAAA AACTTTAAGG
  241 GGTTTTTGGG GCCCCAAAGC CAAGGGGCCC TTTTTAAAAC CCTTTCGGCT AACCCCTTTT
  301 TTTGGGGGAC CCCCCAAAAA TCCCAAAAAA AAAGGGAAAT CCTTTTCTGG GGATTTATTT
  361 AGGACCCCCC AAACCCCAAA AAAAACCCCC TTAAAAAAAA CCCCCCCCCC CCCCCCCCCC
  421 TTTTTCCCCC CCAAATTAAA CAAGGGGGGG GGAAAATTTT TTAAAAAAAC CCTTGGGGAA
  481 ACCCCCCCCC TTGGTTGGGG GTTTTAAAAA AAAATTCCCC CCCCCCCGGC ACAGGGCTTT
  541 GGCCAAAATT TAAGGGGGGT TTTTTTTTTC AAAGGGGGGG AGGGAAGGGG GGCTTGGCAT
  601 CCTGGTTTCA CTCAAATTGG GGAAAACGGT GTCGAACGGC CCTGATTGAG ACCTCTTTAA
  661 CAACTGAGTG AGTTGAACTG ACCAGAGTCA TCGACCGGAT TATGCCTATC CGAATCGACA
  721 AATCTTAGCC TGTGTGAACG ATCACTGGAT GAACGACCAA GTTCGACAAC TGAATCATAT
  781 GGCCCACACC CAGTTAGCAC GTAGTGCACA CGCGATATTG CCGTTGAACT CAAGATAAGT
841 CGCGCTACGA CACGCTGAAC GGAGCGTGGA ACTACTGACC GACTGATGAT
//
Primer: SV40pro_F
Sequence file: RDB12206_A5Hpe.seq check
>12206_12206_A5Hp_SV40pro_F_D09_12.ab1
    1 CACTGTCCTG TACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATTTC ACCGCGCTTT CTATGAAGGT TGGGCTTCGG
 1021 ATCGTTTCGG GACGGCGGCT GATGATCCTC AGCGCGGGAT CTCATGCTGA AGTCCTCGCC
 1081 ACCCACCTGG TTATTGCCAG CTATATGTAC AATTAAGCCA TAGCATCACA ATTTCAACAA
1141 ATAAAGGCCA GT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]