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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Ecad-Flag/pCAG (#RDB12215)

Expression clone of mouse E-cadherin

Clone info. Expression clone of mouse E-cadherin, FLAG-tagged.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 6.1 kb
Gene/insert name Mouse E-Cadherin cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12215 Ecad-Flag/pCAG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Ecad-Flag/pCAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12215).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12215_A3Iip1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: insert 5'(Ecad 5')
Sequence file: RDB12215_A3Iia.seq check
>12215_1_pAxCAF1_B05_05.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNTTCTT CTTTTTCCNA CNNCTCCTGG
   61 GCAACGTGCT GGTTATTGNN NNNNNNNNNN NNTTTGGCAA AGAATTGGTA CCGATGATCT
  121 GCCTTACAAT TAATACATAA CCTTTTGTAT CATACACATA CGATTTAGGT GACACTATAG
  181 AATTCGGGCC GGCCTAACCC GGCCCTGCCC GACCGCACCC GAGCTCAGTG TTTGCTCGGC
  241 GTCTGCCGGG TCCGCCATGG GAGCCCGGTG CCGCAGCTTT TCCGCGCTCC TGCTCCTGCT
  301 GCAGGTCTCC TCATGGCTTT GCCAGGAGCT GGAGCCTGAG TCCTGCAGTC CCGGCTTCAG
  361 TTCCGAGGTC TACACCTTCC CGGTGCCGGA GAGGCACCTG GAGAGAGGCC ATGTCCTGGG
  421 CAGAGTGAGA TTTGAAGGAT GCACCGGCCG GCCAAGGACA GCCTTCTTTT CGGAAGACTC
  481 CCGATTCAAA GTGGCGACAG ACGGCACCAT CACAGTGAAG CGGCATCTAA AGCTCCACAA
  541 GCTGGAGACC AGTTTCCTCG TCCGCGCCCG GGACTCCAGT CATAGGGAGC TGTCTACCAA
  601 AGTGACGCTG AAGTCCATGG GGCACCNNNN NCACCGGCAC CACCACCGCG ACCCTGCCTC
  661 TGAATCCAAC CCAGAGCTGC TCATGTTTCC CAGCGTGTAC CCAGGTCTCN NAAGACAGAA
  721 ACGAGACTGG GTCATCCCTC CCATCAGCTG CCCCGAAAAT GAAAAGGGTG AATTCCCAAA
  781 GAACCTGNTT CANATCNAAT CCAACAGGGA CAAAGAAACN AAGGNTTTCT ACNGCATCAC
  841 CGGCNNANNG AGCTGACAAN NCCCCCGTTG GCGTTTNCAT CNTTGANAGG GAGACAGGNN
  901 TGGCTGAAAA GTGACANNNN NNTCNGGNAT AGAGAANNCN NTTGNCNAAG TACATCCTCN
  961 ANTNCTCATG NCNNNGTCAT CAAANGGGGG AANCNGNNGG NANNNNNCCC NNGGNNNANN
1021 NNNATNNNNN NNNNCANNNC NNANNGNNNA NNNNNNNNNN
//
Primer: pCA-bgter (Pr0066)
Region: insert 3'(FLAG,Ecad 3')
Sequence file: RDB12215_A3Iib.seq check
>12215_12215_A3Ii_1_pCA-bgter_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTTTGGNNNA NGNNNNNGNN CTNNGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT
  121 TGTCGACGAT ATCTTATTTG TCATCGTCGT CCTTGTAGTC TCTAGAGTCG TCCTCACCAC
  181 CGCCGTACAT GTCCGCCAGC TTCTTGAATC GGTTGCCCCA CTCGTTCAGA TAATCGTAGT
  241 CCTGGTCCTG ATCCGACTCA GAGGAGTTCA GTGAGCTCAG GCTAGCGGCT TCAGAACCAC
  301 TGCCCTCGTA ATCGAACACC AACAGAGAGT CGTAAGGGGG TGCCGTGGGG TCGCTGTCGG
  361 CTGCCTTCAG GTTTTCATCG ATGAAGTTTC CAATTTCATC AGGATTGGCA GGACGGGGAC
  421 GATACTGGGG CACGCTCATG AGGGTGGGAG CCACATCATT TCGAGTCACT TCCGGTCGGG
  481 CATCCAGGCC CCTGTGCAGC TGGCTCAAAT CAAAGTCCTG GTCTTCTTCT CCACCTCCTT
  541 CTTCATCATA GTAATACACA TTGTCCCGGG TATCATCATC TGGTGGCAGC AGGGGCTCTT
  601 TGACCACCGT TCTCCTCCGT AGAAACAGTA GGAGCAGCAG GATCAGAATC AGCAGGGCGA
  661 GGATCCCTCC AAGGANTCCG AGGATGGCNA NGAACTTGCA ATCCTGCTGN NNNNATTCCC
  721 GCCTTCATGC AGTTGNTGAN CNGTCCCNTN NNNGTCACAC ACATNGGACG TCCNAANNTG
  781 GNCNCCTGGG TCTTTGTTCT GGTTATCNNC GAGNTTNNAN ANNGNNNTTT TGNANTCGCC
841 NNNNNNNNNN
//
Primer: phRLR1 (Pr0219)
Region: NeoR
Sequence file: RDB12215_A3Iic.seq check
>12215_12215_A3Ii_1_phRLR1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTTGATGNN TTTAGAAAAA TAAACAAATA GGGGTTCCGC
   61 GCACATTTCC CCGAAAAGTG CCACCTGGGT CGAGCAGTGT GGTTTTCAAG AGGAAGCAAA
  121 AAGCCTCTCC ACCCAGGCCT GGAATGTTTC CACCCAATGT CGAGCAGTGT GGTTTTGCAA
  181 GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAAAC
  241 CCCGCCCAGC GTCTTGTCAT TGGCGAATTA ATTCGAACAC GCANATGCAG TCGGGGCGGC
  301 GCGGTCCCAG GTCCACTTCG CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC
  361 CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC
  421 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT
  481 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG
  541 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG
  601 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA
  661 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA
  721 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC
  781 GACNNCCAAG CGAAACANNN NNTCNAGCGN NNANGNACTC GGATGGAAGC CGGTCTTGNC
  841 GATCNNGANG ATCTGGANNA ANANCATCNN GGGGCTCGCG CCNGCCNAAC TNTTCNCCAG
  901 GNNNNNNGGC NCNNATGCCC NANGGNNNNG ATNTCNNCNN GACCCNNGGN NNATGNNNNG
961 CTTGNCNAAT ANCNNNNNNN AAANNNNNNN CNTTNTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]