RDB12894 - pENTR/SV40LT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pENTR/SV40LT (#RDB12894)

Plasmid vector of SV40 large T antigen.

Clone info.	Plasmid vector of SV40 large T antigen. Gateway(R) compatible.
Vector backbone	pENTR (Plasmid)
Selectable markers	Kanamycin
Gene/insert name	SV40 Large T antigen cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12894	pENTR/SV40LT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR/SV40LT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12894).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12894_A8Ajp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531) Region: rrnBT1 ter, attL1, SV40 LT 5' Sequence file: RDB12894_A8Aja.seq
>RDB12894_A8Aj_3_rrnBT1ter_F2_E03_15_ABI24.ab1 1 CTTTTCCCGG ACTGAGCCTT TCGTTTTATT TGATGCCTGG CAGTTCCCTA CTCTCGCGTT 61 AACGCTAGCA TGGATGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTCTTAAGCT 121 CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA 181 TGAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC 241 CTTCACCATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG 301 TCTTGAAAGG AGTGCCTGGG GGAATATTCC TCTGATGAGA AAGGCATATT TAAAAAAATG 361 CAAGGAGTTT CATCCTGATA AAGGAGGAGA TGAAGAAAAA ATGAAGAAAA TGAATACTCT 421 GTACAAGAAA ATGGAAGATG GAGTAAAATA TGCTCATCAA CCTGACTTTG GAGGCTTCTG 481 GGATGCAACT GAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT 541 TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC 601 TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT 661 TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG 721 CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA 781 ATATTCTGTA ACCTTTATAA GTAGGCATAA CAGTTATAAT CATAACATAC TGTTTTTTCT 841 TACTCCACAC AGGCATAGAG TGTCTGCTAT TAATAACTAT GCTCAAAAAT TGTGTACCTT 901 TAGCTTTTTA ATTTGTAAAG GGGTTAATAA GGAATATTTG ATGTATAGTG CCTTGACTAG 961 AGATCCATTT TCTGTTATTG AGGAAAGTTT GCCAGGTGGG TTAAAAGGAG CATGATTTTA 1021 ATCCAGAAAG AAGCAGAGGA AACTAAACAA GTGTCCTGCA AGCTTGTAAC AGAAGTATGC 1081 AATGCAAACA AAATGTGATG ATGTGATGCT AATGCCTTGG GAATGTACTT GCAATTTCAG 1141 GT //
Primer: pDONR_R (Pr0071) Region: T7 pro,attL2, SV40 LT 3' Sequence file: RDB12894_A8Ajb.seq
>RDB12894_A8Aj_3_pDONR_R_F03_18_ABI24.ab1 1 GCCCGACGGG CAGGGAACAA GCTATGACCA TGTAATACGA CTCACTATAG GGGATATCAG 61 CTGGATGGCA AATAATGATT TTATTTTGAC TGATAGTGAC CTGTTCGTTG CAACAAATTG 121 ATAAGCAATG CTTTCTTATA ATGCCAACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC 181 CCTTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC 241 TACAAATGTG GTATGGCTGA TTATGATCAT GAACAGACTG TGAGGACTGA GGGGCCTGAA 301 ATGAGCCTTG GGACTGTGAA TCAATGCCTG TTTCATGCCC TGAGTCTTCC ATGTTCTTCT 361 CCCCACCATC TTCATTTTTA TCAGCATTTT CCTGGCTGTC TTCATCATCA TCATCACTGT 421 TTCTTAGCCA ATCTAAAACT CCAATTCCCA TAGCCACATT AAACTTCATT TTTTGATACA 481 CTGACAAACT AAACTCTTTG TCCAATCTCT CTTTCCACTC CACAATTCTG CTCTGAATAC 541 TTTGAGCAAA CTCAGCCACA GGTCTGTACC AAATTAACAT AAGAAGCAAA GCAATGCCAC 601 TTTGAATTAT TCTCTTTTCT AACAAAAACT CACTGCGTTC CAGGCAATGC TTTAAATAAT 661 CTTTGGGCCT AAAATCTATT TGTTTTACAA ATCTGGCCTG CAGTGTTTTA GGCACACTGT 721 ACTCATTCAT GGTGACTATT CCAGGGGGAA ATATTTGAGT TCTTTTATTT AGGTGTTTCT 781 TTTCTAAGTT TACCTTAACA CTGCCATCCA AATAATCCCT TAAATTGTCC AGGTTATTAA 841 TTCCCTGACC TGAAGGCAAA TCTCTGGACT CCCCTCCAGT GCCCTTTACA TCCTCAAAAA 901 CTACTAAAAA CTGGTCAATA GCTACTCCTA GCTCAAAGTT CAGCCTGTCC AAAGGGCAAA 961 TTAACATTTA AAGCTTTCCC CCCACATAAT TCAAGCAAAG CAGCTGCTAA TGTAGTTTTA 1021 CCACTATCAA TTGGTCCTTT AACAGCCCAG TTATCTTTTT TTTTAGAATG TTGTACACCA 1081 TGCATTTTTA AAAAGTCATA CACACTGAAT CCATTTTGGC ACCAACAGTG TAGCAAGCCA 1141 ACTTCCAAGT CCAACTTCCA ATTTCTTCCT AATGT //
Primer: LargeT antigen innerF1 (Pr0006) Region: SV40 LT mid Sequence file: RDB12894_A8Ajc.seq
>RDB12894_A8Aj_3_LargeT antigen innerF1_C01_03_ABI08.ab1 1 ACTGGGTTTT TTGAGGGAAG TTTGCCAGGT GGGTTAAAGG AGCATGATTT TAATCCAGAA 61 GAAGCAGAGG AAACTAAACA AGTGTCCTGG AAGCTTGTAA CAGAGTATGC AATGGAAACA 121 AAATGTGATG ATGTGTTGTT ATTGCTTGGG ATGTACTTGG AATTTCAGTA CAGTTTTGAA 181 ATGTGTTTAA AATGTATTAA AAAAGAACAG CCCAGCCACT ATAAGTACCA TGAAAAGCAT 241 TATGCAAATG CTGCTATATT TGCTGACAGC AAAAACCAAA AAACCATATG CCAACAGGCT 301 GTTGATACTG TTTTAGCTAA AAAGCGGGTT GATAGCCTAC AATTAACTAG AGAACAAATG 361 TTAACAAACA GATTTAATGA TCTTTTGGAT AGGATGGATA TAATGTTTGG TTCTACAGGC 421 TCTGCTGACA TAGAAGAATG GATGGCTGGA GTTGCTTGGC TACACTGTTT GTTGCCCAAA 481 ATGGATTCAG TGGTGTATGA CTTTTTAAAA TGCATGGTGT ACAACATTCC TAAAAAAAGA 541 TACTGGCTGT TTAAAGGACC AATTGATAGT GGTAAAACTA CATTAGCAGC TGCTTTGCTT 601 GAATTATGTG GGGGGAAAGC TTTAAATGTT AATTTGCCCT TGGACAGGCT GAACTTTGAG 661 CTAGGAGTAG CTATTGACCA GTTTTTAGTA GTTTTTGAGG ATGTAAAGGG CACTGGAGGG 721 GAGTCCAGAG ATTTGCCTTC AGGTCAGGGA ATTAATAACC TGGACAATTT AAGGGATTAT 781 TTGGATGGCA GTGTTAAGGT AAACTTAGAA AAGAAACACC TAAATAAAAG AACTCAAATA 841 TTTCCCCCTG GAATAGTCAC CATGAATGAG TACAGTGTGC CTAAAACACT GCAGGCCAGA 901 TTTGTAAAAA CAAATAGATT TTAGGGCCCA AAGGATTATT TAAAAGCATT TGCCTGAAAC 961 GCAGTGAAGT TTTTTGTTTA GAAAAGAAGA ATAAATTCAA GTGGCAATTG CTTTGCTTTC 1021 CTTAATGTTA AATTTGGTAC AGACCTGTGG CTGGAGTTTG CTCAAGTATT CAGAGCAGGA 1081 TTGGTGAAAG CTGGCAAGAG AAGATTTGGA ACGAAGGAGT TTAAGGGTTT TGTGTTCACG 1141 AG //