RDB12953 - CSIV-CMV-mc-Myc-IRES2-Venus

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-CMV-mc-Myc-IRES2-Venus (#RDB12953)

Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.

Clone info.	Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Mouse Myc cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB12953z.seq
Remarks, protocol and/or map (pdf)	RDB12953.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12953	CSIV-CMV-mc-Myc-IRES2-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-CMV-mc-Myc-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12953).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12953_A8Hrp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' Sequence file: RDB12953_A8Hra.seq
>12953_12953_A8Hr_CSII-CMV5'_G08_20_ABI24.ab1 1 GGGCCGGGCA ATCAAAGGGT TTGGTACAAA AGCAGGCTCC GCGGCCGCCC CCTTCACCAT 61 GCCCCTCAAC GTGAACTTCA CCAACAGGAA CTATGACCTC GACTACGACT CCGTACAGCC 121 CTATTTCATC TGCGACGAGG AAGAGAATTT CTATCACCAG CAACAGCAGA GCGAGCTGCA 181 GCCGCCCGCG CCCAGTGAGG ATATCTGGAA GAAATTCGAG CTGCTTCCCA CCCCGCCCCT 241 GTCCCCGAGC CGCCGCTCCG GGCTCTGCTC TCCATCCTAT GTTGCGGTCG CTACGTCCTT 301 CTCCCCAAGG GAAGACGATG ACGGCGGCGG TGGCAACTTC TCCACCGCCG ATCAGCTGGA 361 GATGATGACC GAGTTACTTG GAGGAGACAT GGTGAACCAG AGCTTCATCT GCGATCCTGA 421 CGACGAGACC TTCATCAAGA ACATCATCAT CCAGGACTGT ATGTGGAGCG GTTTCTCAGC 481 CGCTGCCAAG CTGGTCTCGG AGAAGCTGGC CTCCTACCAG GCTGCGCGCA AAGACAGCAC 541 CAGCCTGAGC CCCGCCCGCG GGCACAGCGT CTGCTCCACC TCCAGCCTGT ACCTGCAGGA 601 CCTCACCGCC GCCGCGTCCG AGTGCATTGA CCCCTCAGTG GTCTTTCCCT ACCCGCTCAA 661 CGACAGCAGC TCGCCCAAAT CCTGTACCTC GTCCGATTCC ACGGCCTTCT CTCCTTCCTC 721 GGACTCGCTG CTGTCCTCCG AGTCCTCCCC ACGGGCCAGC CCTGAGCCCC TAGTGCTGCA 781 TGAGGAGACA CCGCCCACCA CCAGCAGCGA CTCTGAAGAA GAGCAAGAAG ATGAGGAAGA 841 AATTGATGTG GTGTCTGGTG GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC 901 TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC 961 CACGTCTCCA CTCACCAGCA CAAACTACGC CGCACCCCCC CTCCACAAGG ATGACTATCC 1021 AGCTGGCCCA AGAGGGCCAA GTTTGGGACA GTGGCAGGCT CTGAGTCAGA TCAAGCTAAC 1081 ACCGCAGTTG CTCAAGTCCC AAGATCTCAG ACAACGGGAA GGTCA //
Primer: Myc-1169F Sequence file: RDB12953_A8Hrb.seq
>12953_12953_A8Hr_Myc-1169F_H08_23_ABI24.ab1 1 GTACGGAAAG CCCCCAAGGT AGTGATCCTC AAAAAGCCAC CGCCTACATC CTGTCCATTC 61 AAGCAGACGA GCACAAGCTC ACCTCTGAAA AGGACTTATT GAGGAAACGA CGAGAACAGT 121 TGAAACACAA ACTCGAACAG CTTCGAAACT CTGGTGCATA AAAGGGTGGG CGCGCCGACC 181 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG AATTCTGCAG CGGCCGCGGA 241 TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC 301 GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG 361 CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA 421 AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA 481 GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT 541 GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT 601 GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA 661 ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC 721 GGTACACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC 781 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC 841 AAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 901 AAACGGGCCA CAAGTTTCAG CGTGTCCCGG CGAGGGGCGA GGGCGATGCC ACCTACGGCA 961 GCTGGACCCT GAAGCTGATC TGCACACAGC CAGCCTGGCC GTGCCTGGCC CACCGTCGTG 1021 AACCACCTTG GGCCTACCGC CTGCAATGCT TCGCCGGCTA ACCCGACACA TGAAGCAGAC 1081 ACGACTCCAT CCAGCTTCCG TCCA //
Primer: HIVgp_R1 Sequence file: RDB12953_A8Hrc.seq
>12953_12953_A8Hr_HIVgp_R1_A09_03_ABI24.ab1 1 CGGGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG 61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT 121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG 181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC 241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT 301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT 361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA 421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT 481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG 541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT 601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC 661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT 721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT 781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT 841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA 901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTTG GAAAGTCCCG 961 TTGATTTTGG GTGCCCAAAA CAAACTCCCA TTGACGTCAA TGGGGGTGGA GACTTGGAAA 1021 TCCCCGTGAG GTCAAACCGC TATCCACGCC CATTGAATGG TACTGCCCAA AACGCATCAC 1081 CATTGCTATT AGCCGAATGA ACTTAATTAC GGTAGAATGT TACCTTGCCC AAGTTAACGA //
Primer: BGH_rev2 Sequence file: RDB12953_A8Hrd.seq
>12953_12953_A8Hr_BGH_rev2_E09_15_ABI24.ab1 1 CTTGTCGCGG TAGTAGCACG GGGGAGGGGC AAGCAACAGA TGGCTGGCAG ACTAGAAGGC 61 ACAGTGCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT 121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT 181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA 241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA ATAACTTCGT 301 ATAATGTATG CTATACGAAG TTATGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC 361 CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG 421 TCTTAAAGGT ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG 481 CCAGGCACAA TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC 541 TAGGTCTCGA CGGTATCGAT GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG 601 GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC 661 CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC 721 AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC 781 AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC 841 CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT 901 GCCCCAACCA GTGGGGGGTT GCGTCAGCAA ACACAGTGCA CACCACGCCA CGTTTGCCTG 961 ACAACGGGCC ACAACTCCTC ATAAGAGACA GCACCCAGGA TTTATACACG AGAGAAAATG 1021 AAGCCCATAC GGCAGCATAG CATGATACAA GGGCAATTAA GGCAGCGTAT CACATAGCCG 1081 TAAAGAGCAC TAGTTAGATA CAGTCAATCC TTTCAACAAA TTTG //
Primer: IRES-F Sequence file: RDB12953_A8Hre.seq
>12953_12953_A8Hr_IRES-F_D09_12_ABI24.ab1 1 CATCTTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG 121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC 181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG 241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC 301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC 421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG 481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC 541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC 601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC 661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC 721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC 781 GCCGCCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGAATTTC AAGGCCTCTC 841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT ATCAACCTCT GGATACAAAA 901 TTGTGAAAGA TTGACTGGTA TCTACTATGT TGCTCTTTTA CGCTATGTGG ATACGCTGCT 961 TAATGCCTTT GTATCATGCT ATTGCTCCGT ATGCATCAAT TTCTCTCTTG ATAATCCTGG 1021 ATGCTGACTC TTTAGAGGGA TGAGCCGTTG TCAGCAACGG TGGCCGT //
Primer: SV40pro_F Sequence file: RDB12953_A8Hrf.seq
>12953_12953_A8Hr_SV40pro_F_F09_18_ABI24.ab1 1 GCTGGTCCTG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 901 ACGAGCCGGA AAGCATAAAA GTGTAAAGCC TTGGGGTGCC TAATGAGTGA GCTAACTCAC 961 ATTTAATTTG CGTTTGCGCT CACTGCCCGC CTTTCCAGTC GGGAAACCCT TGTCGTGCAG 1021 CCTGCATTTA ATGAATTCGG TCACGCGCGG GAGGAGGCGG TTGCGTAATG GGCGCCTCTT 1081 TCGCATTCTT CGGACTCA //
Primer: Sequence file: RDB12953_A8Hrg.seq
1
Primer: Sequence file: RDB12953_A8Hrh.seq
1