RDB12968 - CS-CDF-PGKG-PRE

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CS-CDF-PGKG-PRE (#RDB12968)

Lentivirus vector plasmid of GFP driven by mouse PGK promoter.

Clone info.	Lentivirus vector plasmid of GFP driven by mouse PGK promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	37C, LB+Amp
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12968zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12968	CS-CDF-PGKG-PRE	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-CDF-PGKG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12968).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12968_A9I4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-M_R (Pr0743) Region: EGFP,PGKpro Sequence file: RDB12968_A9I4a.seq
>12968_12968_A9I4_3_EGFP-M_R_H08_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNCT TGNNGTTNNC CTTGNTGCCG TTCTTCTGCT 61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG 361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT 421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA 481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG 541 GTAGCGCTAG GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC 601 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC 661 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA NCCCAGAAAG 721 CGAANGAGCA AAGCTGCTAT TGGNCGCTGC CCCAAAGGCC TACCCGCTNN CNTTGCTCNN 781 CGGTGCTGTC CATCTGCACN ANNNTAGTGA GACGTGCTAC NTTNCNNTTG TCACGTCCNG 841 CNCGACNCNN NCTGCNGGGN NGGGGGGNNA ACTTTNNNGA CTANGGNANG NNTANNANGN 901 GGNNCNANNG GGNCNNCCNA ANNAACNNNA NNCNGNNGGC GNCNNNCNNN TNGGANGNNG 961 GAAANNGNGN NNNNANGNCC NNAANGNCCA NNTNGTGNNN NCGNCNAANN NNCCNNNNGG 1021 GGGNNNNNNN ANNNNNN //
Primer: PRE_rev (Pr0250) Region: WPRE,EGFP Sequence file: RDB12968_A9I4b.seq
>12968_12968_A9I4_3_PRE_rev_F12_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN NNNGAGCAAC ATAGTTAAGA NTACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG 121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC 361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG 421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTANGTCAGG 661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG 721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGNNCG 781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC 841 TTGCTCACCA TGGTGGCGAC CGGTANCGCT N //
Primer: CMV enh_F1 (Pr0652) Region: CMVpro,5'LTR,HIV-1psi Sequence file: RDB12968_A9I4c.seq
>12968_12968_A9I4_3_CMVenh_F1_G12_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTG CCNNGNANNT GACCTTATGG GANTTTCCTA CTTGGCAGTA 61 CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG 121 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG 181 GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC 241 ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG 301 CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC 541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT 601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT 661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA NNNCAGGGGG 721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC 781 AGTTAATCCT GGCCTGTTAG AAACATCAGA ANGCTGTAGA CAAATACTGN NACAGCTACN 841 ACCATCCCTT CAGACAGGAT CAGAAGAACT TANATC //
Primer: RRE-M_R (Pr0651) Region: RRE,HIV-1psi,5`LTR Sequence file: RDB12968_A9I4d.seq
>12968_12968_A9I4_3_RRE-M_R_H12_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG CTGTTGCGCC TCNATAGCCC TCAGCAAATT GTTCTGCTGC 61 TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC 121 ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT 181 TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT 241 TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA 301 GGTCTGAAGA TCAGCGGCCG CTTGCTGTGC GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC 361 TATCTTGTCT AAAGCTTCCT TGGTGTCTTT TATCTCTATC CTTTGATGCA CACAATAGAG 421 GGTTGCTACT GTATTATATA ATGATCTAAG TTCTTCTGAT CCTGTCTGAA GGGATGGTTG 481 TAGCTGTCCC AGTATTTGTC TACAGCCTTC TGATGTTTCT AACAGGCCAG GATTAACTGC 541 GAATCGTTCT AGCTCCCTGC TTGCCCATAC TATATGTTTT AATTTATATT TTTTCTTTCC 601 CCCTGGCCTT AACCGAATTT TTTCCCATCG CGATCTAATT CTCCCCCGCT TAATACTGAC 661 GCTCTCGCAC CCATCTCTCT CCTTCTAGCC TCCGCTAGTC AAAATTTTTG GCGTACTCAC 721 CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA 781 GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGANT 841 TT //
Primer: YFP_R (Pr0127) Region: EGFP,PGKpro,cPPT_CTS Sequence file: RDB12968_A9I4e.seq
>12968_12968_A9I4_YFP_R_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCTNANTTGT GGCCGTTTNC GTCGCCGTCC NGCTCGACCA 61 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTA 121 GCGCTAGGGT CGAAAGGCCC GGAGATGAGG AAGAGGAGAA CAGCGCGGCA GACGTGCGCT 181 TTTGAAGCGT GCAGAATGCC GGGCCTCCGG AGGACCTTCG GGCGCCCGCC CCGCCCCTGA 241 GCCCGCCCCT GAGCCCGCCC CCGGACCCAC CCCTTCCCAG CCTCTGAGCC CAGAAAGCGA 301 AGGAGCAAAG CTGCTATTGG CCGCTGCCCC AAAGGCCTAC CCGCTTCCAT TGCTCAGCGG 361 TGCTGTCCAT CTGCACGAGA CTAGTGAGAC GTGCTACTTC CATTTGTCAC GTCCTGCACG 421 ACGCGAGCTG CGGGGCGGGG GGGAACTTCC TGACTAGGGG AGGAGTAGAA GGTGGCGCGA 481 AGGGGCCACC AAAGAACGGA GCCGGTTGGC GCCTACCGGT GGATGTGGAA TGTGTGCGAG 541 GCCAGAGGCC ACTTGTGTAG CGCCAAGTGC CCAGCGGGGC TGCTAAAGCG CATGCTCCAG 601 ACTGCCTTGG GAAAAGCGCC TCCCCTACCC GGTAGAATTC AAAGTGAATT TCTGCTGTCC 661 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT 721 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCNNNCT 781 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA 841 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCNN //
Primer: BGH_rev2 (Pr0606) Region: bGHp(A),3'LTR Sequence file: RDB12968_A9I4f.seq
>12968_12968_A9I4_3_BGH_rev2_E12_15_ABI24.ab1 1 NNNNNNNNNN GNNNNNNGGG GGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG 121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC 301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA 361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT 421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT 481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG 541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC 601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT 661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA 721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA 781 CGGTATCGAT GCGGGGNGGC GGNC //
Primer: pAxEF R2 (Pr0059) Region: SV40p(A),Zeo,SV40pro_ori Sequence file: RDB12968_A9I4g.seq
>12968_12968_A9I4_pAxEF R2_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNTNNTTT GTGANNTTNT GNNGCTATTG CTTTATTTGT AACCATTATA 61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA 241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT 301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA 361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC 421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC 481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC 541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT 601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA 661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA 721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGAN //