RDB13081 - pROSA-floxNeo-Fucci2a

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pROSA-floxNeo-Fucci2a (#RDB13081)

Fucci2a cell cycle reporter. Targeting vector for R26 locus

Clone info.	Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120). Targeting vector for R26 locus. Fucci2aR ROSA26 knock-in mice (cat.#RBRC06511) is available from the Experimental Animal Division.
Vector backbone	pCAGiP (Plasmid)
Selectable markers	Ampicillin
Gene/insert name	Human Cdt1 cDNA Human Gem cDNA Corynebacterium diphtheriae Diphtheria toxin A genomic DNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Mort, Richard \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
(3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested. (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested. (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 付加的使用条件： 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
備考	タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13081	pROSA-floxNeo-Fucci2a	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pROSA-floxNeo-Fucci2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13081).

Reference section:

Mort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13081_A5Ajp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: lox P,NeoR Sequence file: RDB13081_A5Ajj.seq
>13081_13081_A5Aj_pAxCA_F1_8_B10_04.ab1 1 CGTCCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT 61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGATATC AAGCTTATCG AGCGGCCGCT 121 CTAGAACTAG TGGATCCATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCCAGATCC 181 CCCGGGCTGC AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC 241 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 301 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 361 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 421 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 481 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 541 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 601 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 661 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 721 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 781 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 841 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 901 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 961 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG 1021 AAATGACCGA CCCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTT CCACCGCGCG 1081 TTCTATGAAA GATGGGGCCT TCGTATCGGT TTCAGGAACG ATGCCTGGAT GATTCCCTCC 1141 AGCCGGCGGG GATTCTCTCT CAAG //
Primer: pAxCALNLF1 (Pr0166) Region: SV40 pA,lox P,mCherry-hCdt1_30_120 5' Sequence file: RDB13081_A5Ajk.seq
>13081_13081_A5Aj_pAxCALNL_F1_8_B01_04.ab1 1 ATTATGTATC TTATCATGTC TGGATCTCCC GATATCCCCA TAACTTCGTA TAGCATACAT 61 TATACGAAGT TATGGATCCC CCGGGCTGCA GGAATTCCTC GACACGCGTA TGGTGAGCAA 121 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA 181 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA 241 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA 301 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT 361 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT 421 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT 481 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA 541 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG 601 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC 661 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT 721 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG 781 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC 841 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG 901 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG 961 CAGAGACTGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCCG AGGCCCCAGA 1021 CATCCCAGCC TGCCCCTTCT CCGGGCCAGA GATAAAGAAT CCACCCCCGG CAGCAGTCAG 1081 CGCTCCCACC TTGAACATCC GCGCAGACAG ACATCAATTC ATGAGGTCGT GCCTTCCTCC 1141 TTCTTTTCAC CTGTATG //
Primer: PGK_N_rev (Pr0243) Region: ROSA_3 5' Sequence file: RDB13081_A5Ajl.seq
>13081_13081_B0I8F_PGK_N_R_G06_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TTATCGATAC CGTCGACGGT 61 ATCGATAAGC TAGATGTCCT GAAATATTTC TATCAGAACA AGGTAGTATA AAGCTGGTAG 121 GTATACAAAA CGCTAGACTA GTTTCTATCC CTGACCCTTA ATCTGCTAGT ATATCCGTAG 181 GAAGTTGCTT AAGTGCCACT AGTACCAACA GCCTCTGTTA GAGTGAACAG TAGATTTTAA 241 TGAAGGGCCA ATAAAGACCA CTTAAGACTA CTGACTGAAT TAAAAATCCA CCCAGTGGAG 301 TTAGTATTGG TTATAGTTAT ATAACAAAAT ATCCAACTTA GCCAGGCGTG GTGGTGCACG 361 CCTTTAATCC CGGCACTCAG GAGGCAGAGG CAGGCGGATT TCTGAGTTCG AGGCCAGCCT 421 GGTCTACAAA GTGAGTTCCA GGACAGCCAG GGCTATACAG AGAAACCCTG TCTCGAAAAA 481 CCAAAAAAAA AAAAAAAAAG GAAAAAAAAT ATGGCTTGAA AACATGCTAT TGTTGTCAAA 541 AGGAGATCCA AACAAGTATT TCTTTGAACA AAAATTATGT AGAAAACAGG GTGATAAAGA 601 ACAAGGGCTT TTTAAAAAAG GCCAGCATTA TTTGAGATAT ACAAGTGACT CCAATTCAAG 661 CCTAAAGGCC ACTCAATGCT CACTAACAGT GCTAATCANA ATCAATTCCT TAGCCCAGTC 721 NAAAATAGTC CTGAATATCT TCTGGATNAA CNTGTCCTCC NACATATNAA NNTANTATAC 781 ACTAANNAAA CGTCTCNACT TNNNNGTGAA ATGCTTGACN CCTANACTTN NGACCNNNNN 841 AANTGCTCTA NNNGTAGGGT TACTANGNNA NANANTCNTG NNNNGNNAAN NNCNNNTATN 901 NNANTNNNNN NNAANNNNAN AANNNGNAAA ANTGANNTNN NTTANNTNNN NTTTNNAAAN 961 NTNAAAANNN NNNNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: ROSA_5 3' Sequence file: RDB13081_A5Ajm.seq
>13081_13081_B0I8F_pUC-M13_-100_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNN GGGTTTTCCC NGTCACGACG TTGTAAAACG 61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGC 121 AGGCCCTCCG AGCGTGGTGG AGCCGTTCTG TGAGACAGCC GGGTACGAGT CGTGACGCTG 181 GAAGGGGCAA GCGGGTGGTG GGCAGGAATG CGGTCCGCCC TGCAGCAACC GGAGGGGGAG 241 GGAGAAGGGA GCGGAAAAGT CTCCACCGGA CGCGGCCATG GCTCGGGGGG GGGGGGGCAG 301 CGGAGGAGCG CTTCCGGCCG ACGTCTCGTC GCTGATTGGC TTCTTTTCCT CCCGCCGTGT 361 GTGAAAACAC AAATGGCGTG TTTTGGTTGG CGTAAGGCGC CTGTCAGTTA ACGGCAGCCG 421 GAGTGCGCAN NCGCCGGCAG CCTCGCTCTG CCCACTGGGT GGGGCGGGAG GTAGGTGGGN 481 N //
Primer: T3 (Pr0112) Region: bGHpA,Diphtheria toxin A 3' Sequence file: RDB13081_A5Ajn.seq
>13081_13081_A5Aj_T3_8_C01_07.ab1 1 ATGTCGGGAC CCCCTCGAGC CCCAGCTGGT TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC 61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC 121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA 181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA 241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGGATC 301 TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT GGAATCTAAA ATACACAAAC 361 AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT TTTATATTTA CCTTAGAGCT 421 TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA CAGAAGTAAG GTTCCTTCAC 481 AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC CATATACTCA TACATCGCAT 541 CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG TTCTACGCTT AACGCTTTCG 601 CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC CTCAGCGAAG GGAAGGCTGA 661 GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA CTCTTCCGTT CCGACTTGCT 721 CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT AATAGTTTCG GCATTATCCA 781 CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC TTTGACCACG CCTCCAGCTT 841 TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC AGCGTCGTAT TTATTGTCGG 901 TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG TGTACCAGAT TTTGGCTTTT 961 GTATACCTTT TTTGAATGGA AATCTACATA ACCAGGGTTT AGTCCCGTGG TACGAAGAAA 1021 AGTTTTCCAT CACAAAAGAT TTAGAGATCA CACATCATCA GGATCATGCG AGACTGCAGT 1081 CGAAGGACAG AGATGAGGAA GAGAGAACAG CGCGCCAGGA CGTGCGCCTT TTTGAAGCGC 1141 GGGTGCA //
Primer: CMV_en_R (Pr0173) Region: ROSA_3 3' Sequence file: RDB13081_A5Ajo.seq
>13081_13081_A5Aj_CMV_en_R_8_A10_01.ab1 1 GTCTTTATTG ATTACTATTA ATAACTAGTC AATAATCAAT GTCGCACTTA ATTAAACGCG 61 CCGGCTAGAA GATGGGCGGG AGTCTTCTGG GCAGGCTTAA AGGCTAACCT GGTGTGTGGG 121 CGTTGTCCTG CAGGGGAATT GAACAGGTGT AAAATTGGAG GGACAAGACT TCCCACAGAT 181 TTTCGGTTTT GTCGGGAAGT TTTTTAATAG GGGCAAATAA GGAAAATGGG AGGATAGGTA 241 GTCATCTGGG GTTTTATGCA GCAAAACTAC AGGTTATTAT TGCTTGTGAT CCGCCTCGGA 301 GTATTTTCCA TCGAGGTAGA TTAAAGACAT GCTCACCCGA GTTTTATACT CTCCTGCTTG 361 AGATCCTTAC TACAGTATGA AATTACAGTG TCGCGAGTTA GACTATGTAA GCAGAATTTT 421 AATCATTTTT AAAGAGCCCA GTACTTCATA TCCATTTCTC CCGCTCCTTC TGCAGCCTTA 481 TCAAAAGGTA TTTTAGAACA CTCATTTTAG CCCCATTTTC ATTTATTATA CTGGCTTATC 541 CAACCCCTAG ACAGAGCATT GGCATTTTCC CTTTCCTGAT CTTAGAAGTC TGATGACTCA 601 TGAAACCAGA CAGATTAGTT ACATACACCA CAAATCGAGG CTGTAGCTGG GGCCTCAACA 661 CTGCAGTTCT TTTATAACTC CTTAGTACAC TTTTTGTTGA TCCTTTGCCT TGATCCTTAA 721 TTTTCAGTGT CTATCACCTC TCCCGTCAGG TGGTGTTCCA CATTTGGGCC TATTCTCAGT 781 CCAGGGAGTT TTACAACAAT AGATGTATTG AGAATCCAAC CTAAAGCTTA ACTTTCCACT 841 CCCATGAATG CCTCTCTCCT TTTTCTCCAT TTATAAACTG AGCTATTAAC CATTAATGGT 901 TTCCAGGTGG ATGTCTCCTC CCCCAATATT ACCTGATGTA TCTTACATAT TGCCAGGCTG 961 ATATTTTAAA GACATTAAAA GGTATATTTC ATTATTGAGC CACATGGGTA TTGATTACTG 1021 CTTTACTAAA ATTTTGTCAT TGTACACATC TGTAAAAGGT GGTTTCCCTT TTGGGAATGC 1081 AAAGTTCAGG TGATTGATGT CTTTCTGACC TTACGTCTGT GAGCCTGCAT TTTTTTCTAA 1141 TTTAAGCAGG GTGCTTTTCT T //
Primer: EGFP-C (Pr0025) Region: mVenus-hGeminin1_110 3',bGHpA,ROSA_5 Sequence file: RDB13081_A5Ajp.seq
>13081_13081_A5Aj_EGFP_C_8_D01_10.ab1 1 GCGTACTCTC GGCATGGACG AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA 61 GATGAATCCC AGTATGAAGC AGAAACAAGA AGAAATCAAA GAGAATATAA AGAATAGTTC 121 TGTCCCAAGA AGAACTCTGA AGATGATTCA GCCTTCTGCA TCTGGATCTC TTGTTGGAAG 181 AGAAAATGAG CTGTCCGCAG GCTTGTCCAA AAGGAAACAT CGGAATGACC ACTTAACATC 241 TACAACTTCC AGCCCTGGGG TTATTGTCCC AGAATCTAGT GAAAATAAAA ATCTTGGAGG 301 AGTCACCCAG GAGTCATTTG ATCTTATGAT TAAAGAAAAT CCATCCTCTC AGTATTGGAA 361 GGAAGTGGCA GAAAAACGGA GAAAGGCGCT GTAAGGTACC ACATGTGGCG CGCGCGCCTT 421 CGATGATCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 481 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 541 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 601 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT 661 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCTGCA GCCCAAGCTT GGGCTGCAGG 721 GCGCGCCCGA TTAAGGCTAG AAAGACTGGA GTTGCAGATC ACGAGGGAAG AGGGGGAAGG 781 GATTCTCCCA GGCCCAGGGC GGTCCTCAGA AGCCAGGAGG CAGCAGAGAA CTCCCAGAAA 841 GGTATTGCAA CACTCCCCTC CCCCCTCCGG AGAAGGGTGC GGCCTTCTCC CCGCCTACTC 901 CACTGCAGCT CCCTTACTGA TAACAACTCA GAGCGACTTT GGGAGAGCAA GTGCTTCCTG 961 CCTCCAAAAC AGCCCAACTG AGCCCTCGCT CCTTTCCTCC ACTCCCCGGA GTGCGCGATG 1021 GGAGGTCTGG CTCAGCACGC CCCTCTTGAG GCAACTCAAG TCGGGAAACG TGCTGCACCC 1081 GCCCCGCAGC GCTCAGCCTT ACTGACGTTC CGCCCCAGCG CGCGCATCTG ACCACGTTGC 1141 CGCCAAGGGG GACGTC //
Primer: EGFP-N (Pr0027) Region: mVenus-hGeminin1_110 5',T2A,mCherry-hCdt1_30_120 Sequence file: RDB13081_A5Ajq.seq
>13081_13081_A5Aj_EGFP_N_8_A01_01.ab1 1 GGCCCGGCGA CGCTCCTCGC CCTTGCTCAC CATGGGTCCA GGATTCTCTT CGACATCGCC 61 ACAAGTGAGA AGAGAGCCAC GGCCTTCCAA TTGGATGGTG TCCTGGTCCT GCGCGGATGT 121 CAGGTGGGGC GGCTGACCTG CTGCCGGGGT GGATTTCTTT ATCTTCTGGC CCGGAGAAGG 181 GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT ACTGGGGCTG GAAACCTCGT CCACCGACAG 241 CCGCAGTCTC CTGCGGGCCG GTGGCCTGGC CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG 301 GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG 361 GCTGGGCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC 421 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG 481 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT 541 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT 601 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC 661 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG 721 CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC 781 GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT 841 CAAGTAGTCG GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT 901 GAGGGGACAG GATGTCCCAG GCGAAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG 961 GCGGTCTGGG TGCCCTCGTA GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC 1021 CGTTCACGGG AGCCCTCCCA TGTGCACCTT TGAGCGCATG AACTCATGAA TGATGGCATG 1081 ATATCTCCTC GCCTGCTCAC ATACGCGTGT CGAGAGTTCC CTGCCAGCCC GGGGGGAATC 1141 CAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP]
reference	Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP]
reference	Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
reference	Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
reference	Glover, J.D., Maintenance of distinct melanocyte populations in the interfollicular epidermis. Pigment Cell Melanoma Res. 28 (4): 476-480 (2015). PMID 25847135.