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pAAV-Enk-FtTA

AAV vector of FLAG-tTA under the control of Enkephalin promoter

Catalog number RDB13211
Resource name pAAV-Enk-FtTA
Alternative name V-E-tTA
Clone info. AAV vector of FLAG-tTA under the control of Enkephalin promoter.
Vector backbone pAAV (Plasmid)
Gene/insert name Mouse Enkephalin promoter genomic DNA
Depositor|Developer Nakanishi, Shigetada |
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External Database
Mouse Enkephalin promoter

          Reference sequence
            
           
          Sequence (full) RDB13211zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 66: 896-907, 2010) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 66: 896-907, 2010)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

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          RDB13211 pAAV-Enk-FtTA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAAV-Enk-FtTA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13211).

          Reference section:

          Hikida, T., Kimura, K., Wada, N., Funabiki, K., Nakanishi, S., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Expression regulation (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13211_A5Cip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAAV-MCS_F1 (Pr0766)
          Region: Enk pro 5'
          Sequence file: RDB13211_A5Cia.seq check
          >D02088F1_A5Ci_1_pAAV-HCS_F1_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCTGTG TGNTGGCCCN TCACTTTGGC AAAGAATTGG
             61 GANTCGAACA TCGATTAGCG ACCAAAATGG GAGACGCACT TCAGATTGCG TCTCTCAGGA
            121 GCTGCGTGAA GGCAGGAACT GCAAAGATTG AAAAGGGTGG AGGATCCCTT GCAAATGTCT
            181 GGGAAAGCCT TGAGACCCTC TCTTTGTTGT AGCCTATTTG GACTAGGAAG ATAGGTACAC
            241 ACAATGGGTA AATCTTTGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG
            301 AGAGAGAAGA TAACTGGTAA CAGATCCAAA GGAGAGGTGG TTTAAAACAG CTTTTGAGGC
            361 CAGACGGGTT AAATGGTTTC GTTCATTATT CCAGATGATT CTGTCCTAAA CACTGTGCCT
            421 CCTCTCTCGG GTACTGACAT AAAATAGCAT GTTACCTCGG CTGCTGTTCT ACAAATAGGA
            481 GAAAAAAAAA AGATGAGCGC TTGGAGTCTA ACTAAGGGTT AGACGATCTA ATCTAAGCTT
            541 CCTGAGTGGC ACCTATCTAC AGACCCTGGA AGGGAGGGAG TGGGCTGCTC TAAAAGCCCC
            601 AGCCTCACCT TTTGGAGCCA GGCCTCTCCT GGAGGACAAG ATGTGCAAGA CCTAAGCCAC
            661 TTGTGTCCAG AACAGCTTTC CTCCTTGTTC CCTCCAAACC ACACTCCTGC TTACTGACAT
            721 CACAACTCCC TGACACCCCT TAATCAACAC ACACACACAC ACACACACAC ACACACACAC
            781 ACACACACAC ACACACGAAA AAGAGCCCAC TCTATACAGC ACTCTGGCTA CAGCAAGTTC
            841 GGANCCCATG NCCCCCCAGC CCGAANNNGA TCCCTCCTGN GGNTCCNANA NGANACACTC
            901 CCCACAANCG NNANTTTGNT GGNTTGNNTG NGTGCTNGTT TTTGANNNTG NCCGTTTGAN
            961 GGTGNNGNTT NGNNCTGNTT CTGNNNNNNN ANNNNNNGNT TTTGNANTNN GNCNNTGNTN
          1021 TNANNAANNN NNTTTNNNAT NCNNNNAAAG TTTCNNNNNN NNNN
          //
          Primer: hGHpolyA-R (Pr0350)
          Region: hGH p(A), tTA 3'
          Sequence file: RDB13211_A5Cib.seq check
          >D02088F1_A5Ci_1_hGHpolyA-R_C10_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NAATANTATA GAAGGNNACC TAGTCAGACA AAATGATGCA
             61 ACTTAATTTT ATTAGGACAA GGCTGGTGGG CACTGGAGTG GCAACTTCCA GGGCCAGGAG
            121 AGGCACTGGG GAGGGGTCAC AGGGATGCCA CCCGTAGATC TCTCGAGCTA CCCACCGTAC
            181 TCGTCAATTC CAAGGGCATC GGTAAACATC TGCTCAAACT CGAAGTCGGC CATATCCAGA
            241 GCGCCGTAGG GGGCGGAGTC GTGGGGGGTA AATCCCGGAC CCGGGGAATC CCCGTCCCCC
            301 AACATGTCCA GATCGAAATC GTCTAGCGCG TCGGCATGCG CCATCGCCAC GTCCTCGCCG
            361 TCTAAGTGGA GCTCGTCCCC CAGGCTGACA TCGGTCGGGG GGGCCGTCGA CAGTCTGCGC
            421 GTGTGTCCCG CGGGGAGAAA GGACAGGCGC GGAGCCGCCA GCCCCGCCTC TTCGGGGGCG
            481 TCGTCGTCCG GGAGATCGAG CAGGCCCTCG ATGGTAGACC CGTAATTGTT TTTCGTACGC
            541 GCGCGGCTGT ACGCGGACCC ACTTTCACAT TTAAGTTGTT TTTCTAATCC GCATATGATC
            601 AATTCAAGGC CGAATAAGAA GGCTGGCTCT GCACCTTGGT GATCAAATAA TTCGATAGCT
            661 TGTCGTAATA ATGGCGGCAT ACTATCAGTA GTAGGTGTTT CCCTTTCTTC TTTAGCGACT
            721 TGATGCTCTT GATCTTCCNA TACGCAACCT AAAGTAAAAT GCCCCACAGC GCTGANTGCA
            781 TATAATGCAT TCTCTAGTGA AAAACCTTGT TGGCATAAAA AGGCTAATTG ATTTTCGAGA
            841 GTTTCATACT GTTTTTCTGT ANGCCGNGTA NCTANNTGTA CTTTTTGCTN CNTCGCGATG
            901 ANTTANTAAA GCANATCNAA AACTTTTANC GTTANTACGN NAAAAANTCN NGNCANCTTN
          961 CCNNNNNTAA ANGGNNAAAA NNGANNATNG NNNNNANNNN NNNNNN
          //
          Primer: TetR-N_R (Pr0764)
          Region: tTA 5', FLAG, Enk pro 3'
          Sequence file: RDB13211_A5Cic.seq check
          >D02088F1_A5Ci_1_TetR-N_R_B10_04_ABI24.ab1
              1 NNNNNNNNNN TTNNNNNNNG NNNNTACNAC GTAGGCTGCT CTACNCCTAG CTTCTGGGCG
             61 AGTTTACGGG TTGTTAAACC TTCGATTCCG ACCTCATTAA GCAGCTCTAA TGCGCTGTTA
            121 ATCACTTTAC TTTTATCTAA TCTAGATGGA TCCTTGTCAT CGTCGTCCTT GTAGTCGAGA
            181 TCCCCCGTCT CTTGTTCCAT GGTGGCGCTA GTAACGGCCG CCAGTGTGCT GGAATTCGCC
            241 CTTGCCTCAA TGAGAGCTTG GACCCTAGTG TTTCCTGCTG TTCTAGTGAT GCCTGGGACT
            301 ATTCTATCTT CCTGGAGGTA TCCTATCTTC CCACGGGGAG TCCTGTTTTC CTGATGACAT
            361 CCTGTCCTTC CGAGGGCGCC CTGTCTTCCC TGGCGTCGGT TGCTTAGGGC AAGATTCTGG
            421 AGCGAACACT GAAGTTCGCG GCTCGGGAGC AGGCGCTTGG GGGAATAGGA CCCGGACACC
            481 CAAAGCAATG AGGAACCCCG AGAAAGTTGG ACGAACGCAT GCACCGAGGG AACAGGCGAC
            541 GGTCGCCAAG GCACGGGCGA CTTGGGGCAA AGTCACTCAC GCTGCCGTTG CTCCAGATGG
            601 CGCAGGTTAC TGGAAGAGCG TGTACCAGAT GCCACTCTGC AAGAGAAAGA AAGAAGGCAA
            661 GTGTAAAGCG GCCGCGTCGG GGAGCTCTGC GAGGCGCCGG GCAGCGTCGC TGGGCAGCGG
            721 GGCTCTGTGC GCCTGCCTTG CTGGCGGCGG CCACAGCCAC TTTATAATTA ACCCCAAACC
            781 GAANGANGCG CGCGCGCCCC NATCGCCNGC GGGCTGCAGC TGANGCANGC CCTACGCCNA
            841 CCCGCGCCGA CGCCTANCGG GANGGGANGT CCCCCCCAGC ACGCGGCCCC NANGGNGGCN
            901 GGNGCGANNG GGCGCCNNNN GNNNCNCCNA CCNANCANTT ANGNNNTCNN GGGGNNGNNN
          961 NGNANNGGNA AACTGNNTTC CNNNCNNNNN NNNNTNNNNN NNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, Enk pro 5'
          Sequence file: RDB13211_A5Cid.seq check
          >D02088F1_A5Ci_1_CMV_Forward_A10_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNGCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
            121 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGCGGA TTCGAATCCC GGCCGGGAAC
            181 GGTGCATTGG AACGCGGATT CCCCGTGCCA AGAGTGACGT AAGTACCGCC TATAGAGTCT
            241 ATAGGCCCAC AAAAAATGCT TTCTTCTTTT AATATACTTT TTTGTTTATC TTATTTCTAA
            301 TACTTTCCCT AATCTCTTTC TTTCAGGGCA ATAATGATAC AATGTATCAT GCCTCTTTGC
            361 ACCATTCTAA AGAATAACAG TGATAATTTC TGGGTTAAGG CAATAGCAAT ATTTCTGCAT
            421 ATAAATATTT CTGCATATAA ATTGTAACTG ATGTAAGAGG TTTCATATTG CTAATAGCAG
            481 CTACAATCCA GCTACCATTC TGCTTTTATT TTATGGTTGG GATAAGGCTG GATTATTCTG
            541 AGTCCAAGCT AGGCCCTTTT GCTAATCATG TTCATACCTC TTATCTTCCT CCCACAGCTC
            601 CTGGGCAACG TGCTGGTCTG TGTGCTGGCC CATCACTTTG GCAAAGAATT GGGATTCGAA
            661 CATCGATTAG CGACCAAAAT GGGAGACGCA CTTCAGATTG CGTCTCTCAN GAGCTGCGTG
            721 AAGGCAGGAA CTGCAAAGAT TGAAAAGGGT GGANGATCCC TTGCAAATGT CTGGGAAAGC
            781 CTTGAGACCC TCTCTTTGTT GTAGCCTATT TGGACTANGA AGATAGGTAC ACACAATGGG
            841 TAAATCTTTG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAA
            901 GANNNCTGGN NNCAGATNNN AAGGAGANNN GGNTTAAAAC AGCTTTTGNN NNCNGANNGG
            961 NTNAAATGNT TTCGTTCATN NNTNNNNANG ANTNTGNNNN NAACNNNGNG NNNNNNNNNN
          //
          Primer: ColE1ori_F (Pr0393)
          Region: ori, AAV2 ITR
          Sequence file: RDB13211_A5Cie.seq check
          >D02088F1_A5Ci_1_ColE1ori_F_D10_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTCTGNNT TGANCGTCGA NTTTTGTGAN GCTCGTCAGG
             61 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
          121 CTGGCCTTTT GCTCACATGT CCTGCAGGCA GCTGCGCGN
          //
          Primer: f1ori_F (Pr0280)
          Region: f1 ori, AAV2 ITR
          Sequence file: RDB13211_A5Cif.seq check
          >D02088F1_A5Ci_1_f1ori_F_E10_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNN NGNAGCGGTC NNGCTGCGCG TAACCNCCAC ACCCGCCGCG
             61 CTTAATGCGC CGCTACAGGG CGCGTACTAT GGTTGCTTTG ACGTATGCGG TGTGAAATAC
          121 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCC CTGCAGGCAG CTGCGCGNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hikida, T., Distinct roles of synaptic transmission in direct and indirect striatal pathways to reward and aversive behavior. Neuron 66 (6): 896-907 (2010). PMID 20620875. [link to RRC of NBRP]

          2024.03.26

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