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pGEX6P-eRF3

Expression vector of human eRF3

Catalog number RDB13270
Resource name pGEX6P-eRF3
Clone info. Expression vector of human eRF3.
Comment Expression was confirmed by depositor with Western blot and CBB staining. cDNA fragment 663..2162 of NM_002094.3 is cloned. Known difference (at least): C to G substitution at 2,003nt (Val, synonym), A to G substitution at 2,081nt (Val, synonym).
Vector backbone pGEX6P (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human eRF3 cDNA
Depositor|Developer Imataka, Hiroaki |
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External Database
Human eRF3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13270 pGEX6P-eRF3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13270_A7Drp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB13270_A7Dra.seq check
          >13270_13270_A7Dr_pGEX-F2_D10_10_ABI24.ab1
              1 GTAGTAGTTT GTTTTAAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC
             61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
            121 TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCC CGGAATTCAT
            181 GGAACTTTCA GAACCTATTG TAGAAAATGG AGAGACAGAA ATGTCTCCAG AAGAATCATG
            241 GGAGCACAAA GAAGAAATAA GTGAAGCAGA GCCAGGGGGT GGTTCCTTGG GAGATGGAAG
            301 GCCGCCAGAG GAAAGTGCCC ATGAAATGAT GGAGGAGGAA GAGGAAATCC CAAAACCTAA
            361 GTCTGTGGTT GCACCGCCAG GTGCTCCTAA GAAAGAGCAT GTAAATGTAG TATTCATTGG
            421 GCACGTAGAT GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT
            481 TGACAAAAGG ACGCTTGAAA AGTATGAAAG AGAAGCTAAA GAGAAAAACA GAGAAACTTG
            541 GTACTTGTCT TGGGCCTTAG ACACAAATCA GGAAGAACGA GACAAGGGTA AAACAGTAGA
            601 AGTGGGTCGT GCCTATTTTG AAACCGAAAA GAAGCATTTC ACAATTCTAG ATGCCCCTGG
            661 CCACAAGAGT TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGATT TGGCTGTGCT
            721 GGTAATCTCA GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG
            781 AGAACATGCA ATGTTGGCAA AGACAGCAGG TGTAAAACAC CTAATTGTGC TAATTAATAA
            841 GATGGATGAT CCAACAGTAA ATTGGAGCAA TGAGAGATAT GAAGAATGTA AGGAGAAACT
            901 AGTGCCATTT TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCACT TTATGCCCTG
            961 CTCAGGACTT ACTGGAGCAA ATCTCAAAGA GCAGTCGGAT TTCTGTCTTG GGTACATTGG
           1021 GATTTACCGT TTATTCATAT CTTGGATTAT TTGCCGAACT CCATTAGATC AGGTGATGGG
          1081 ACCAATCAGC TGCTATGATG ATAGTTACAA GGAATATGGG CAACCTGATG G
          //
          Primer: pGEX-R
          Sequence file: RDB13270_A7Drb.seq check
          >13270_13270_A7Dr_pGEX-R_E10_13_ABI24.ab1
              1 CGGGTTAATC GGGAACGCGC GAGGCGGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT
             61 TAGTCTTTCT CTGGAACCAG TTTCAGAACT TTTCCAATTG CAATGGTCTT ACCCTCATCT
            121 CTTAAGGTGA AACGACCCAT CTGAGGGAAG TCTTTAAAGG TCTCAAGGCA GATGGTTCCT
            181 GCTGTCCTTA AGCGAGCAAT GCACACTTGA TCTTGTTTCA CAAAACGGGG TCGGGTCTTA
            241 CTTTTTTCTC CTGATTTTTT GTCTACCAAG CAGATTAAGG CTGTTATTTC CACCTCCTCA
            301 ATACAGGTAT GAATATGCAG CACCGCATTA TAGCCTGGGC AGATGATGGA TTTGTGCTCT
            361 ATAATCACTA TCTGGGCATC AAATGTGCGT CCAGAATGAC AAAGATTATT AGGATCACAA
            421 AGTATAAACC CTGGAAGAAT CTCCTCTTCT TCAATTCCTT TCAGTCTGAT TTTGAGGTTT
            481 TCACCTGGGG CTACGGTATC AGTCTCTACA TCATCGGAAA GTATTCCAAG AACTTCCACG
            541 TTGTGCTTGT TTGGCATCAT CACAAGCTGC TGGCCTTTAC AAATAGATCC TGATTCCAGC
            601 TTTCCCAGGA CCACAGTGCC CATATCCTTG TACTTATCCA CAATTGGCAG CCTGATTGGT
            661 CCATCAACTG ATCTATTGAA GTTCGGCAAA TTATCCAGAT ATGGAATAAA CGGTAATCCA
            721 ATGTACCAAG GACAGAAATC CGACTGCTCT TTGAGATTTG CTCCAGTAAG TCCTGAGCAG
            781 GGCATAAAGT GAATGTCCTT TTTGGGATTG AAGCCAACTT TTTTTCAAAA ATGGCACTAG
            841 TTTCTCCTTA CATTCTTCAT ATCTCTCATT GCTCCAATTT ACTGTTGGAT CATCCATCTT
            901 ATTAATTAGC ACAATTAGGT GTTTTACACC TGCTGTCTTT GCCAACATTG CATGTTCTCT
            961 TGTCTGTCCT CCTTTTTTCA AATCCAGTTT CAACTCTCCT TTCCCTGGCT GAGATACCAG
           1021 CACAGCCCAA ATCAGCTGGA GAGCACACGA TCATATTTGG GACCAAACTC TGTGCAGGGG
          1081 CATCTAGATG TCAAATGCTT CCTTTTCCGA TTCAAAATAG GCCACGGATT CT
          //
          Primer: pGEX-ptac_up-F
          Sequence file: RDB13270_A7Drc.seq check
          >13270_13270_A7Dr_pGEX-ptac_up-F_F10_16_ABI24.ab1
              1 AAAAGCCCCG CTCATAACGG TTCTGGCAAT ATTCTGAAAT GAGCTGTTGA CAATTAATCA
             61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT
            121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT
            181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA
            241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA
            301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA
            361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT
            421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT
            481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA
            541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA
            601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA
            661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC
            721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC CAAAATCGGA
            781 TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCCCGGAA TTCATGGAAC TTTCAGAACC
            841 TATTGTAGAA AATGGAGAGA CAGAAATGTC TCCAGAAGAA TCATGGGAGC ACAAAGAAGA
            901 AATAAGTGAA GCAGAGCCAG GGGGTGGTTC CTTGGGAGAT GGAAAGGCCG CCAGAGGAAA
            961 GTGCCCATGA AATGATGGGA GGAGGAAGAG GAAATCCCAA AAACCTAAAG TCTGTGGTTG
           1021 CACCGCCCAG GGTGCTCTAG AAAGAGCATG TAAATGTAGT ATCATGGGGC ACGTAGATGC
          1081 TGCAAGGTCA ATCAATGGGA GACAAATTAA TGCTAATTTG AACCTGGG
          //
          Primer: M13_-40
          Sequence file: RDB13270_A7Drd.seq check
          >13270_13270_A7Dr_M13(-40)_G10_19_ABI24.ab1
              1 CAGGCGCGAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
             61 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
            121 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
            181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
            241 AGCGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG
            301 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
            361 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT
            421 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC
            481 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC
            541 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA
            601 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
            661 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG
            721 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG
            781 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
            841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC
            901 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
            961 AGTTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGCAGAC
           1021 TGGAGTTGCC ACCGCATCAG CCACGACTGT TTGGCCCGCA GCTGGTGTGC ACGGCGTTTG
          1081 GAATGTATCA GCTTCCGGCA TCGACGCTTC AACTTTTTGC CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
          reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl