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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-SOD1-FLAG_G93A (#RDB13388)

Mammalian expression vector for human G93A SOD1 with FLAG tag at C-terminus.

Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly93Ala mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly93Ala mutation is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13388 pcDNA3-SOD1-FLAG_G93A DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-SOD1-FLAG_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13388).

Reference section:

Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13388_A5Edp1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert(SOD1 G93A),Flag,bGH pA
Sequence file: RDB13388_A5Eda.seq check
>13388_13388_A5Ed_2_CMV_Forward_G04_19.ab1
    1 CAACACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACTAGTAAC GGCCGCCAGT
  181 GTGCTGGAAT TCTGCAGAAT CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT
  241 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG
  301 GGAAGCATTA AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT
  361 AATACAGCAG GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT
  421 GGGCCAAAGG ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT
  481 GCTGTGGCCG ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC
  541 ATTGGCCGCA CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA
  601 GAAAGTACAA AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC
  661 CAAGACTACA AAGACGATGA CGACAAGTGA GATATCCATC ACACTGGCGG CCGCTCGAGC
  721 ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC
  781 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
  841 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
  901 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGG
  961 AGGATTGGGA AAGACAATAG CAGGCATGCT GGGGGATGCG GGTGGGCTCT ATGGGCTTCT
 1021 GAGGCGGGAA AGAACCCAGC TGGGGGCTCT AGGGGGTATC CCCCACGCGC CCTGTAGCCG
 1081 CGCATTAAGC GCGGCGGGCT GTGATGTAAC GCCGCAAGCG GTGACCGCTA CACTTGCCAG
1141 GCGCCCCTTA AGCGG
//
Primer: SV40pro_F (Pr0110)
Region: Sv40 pro_ori,Neo R
Sequence file: RDB13388_A5Edb.seq check
>13388_13388_A5Ed_2_SV40pro_F_H04_22.ab1
    1 CCTGTAACTA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
 1021 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCCAGCGCGG GGATCTCATG CTTGGAGTCC
 1081 TCGCCCACCC CACCTTGATA TGCAGCTATA TGCTACAATA AGGCATAGCC ATCCACAAAT
1141 TTCCACAAAA TTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]