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pcDNA3.1/hGnT-V-myc-his (#RDB13428)

Expression vector of human GnT-V in mammalian cell

Clone info. Expression vector of human GnT-V in mammalian cell.
Comment Exression was confirmed by the depositor with Western blotting and enzymatic activity asssay.
Vector backbone pcDNA3.1/Myc-His version C (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human MGAT5 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem, 288 (39), 27912-27926 (2013))
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem, 288 (39), 27912-27926 (2013))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13428 pcDNA3.1/hGnT-V-myc-his DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1/hGnT-V-myc-his was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13428).

Reference section:

Korekane, H., Park, J.Y., Matsumoto, A., Nakajima, K., Takamatsu, S., Ohtsubo, K., Miyamoto, Y., Hanashima, S., Kanekiyo, K., Kitazume, S., Yamaguchi, Y., Matsuo, I., Taniguchi, N., Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). J. Biol. Chem. 288 (39): 27912-27926 (2013). PMID 23960081. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13428_A6Dqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB13428_A6Dqa.seq check
>D02340A2_A6Dq_3_CMV_Forward_H01_22.ab1
    1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCATGGCTCT CTTCACTCCG
  181 TGGAAGTTGT CCTCTCAGAA GCTGGGCTTT TTCCTGGTGA CTTTTGGCTT CATTTGGGGT
  241 ATGATGCTTC TGCACTTTAC CATCCAGCAG CGAACTCAGC CTGAAAGCAG CTCCATGCTG
  301 CGCGAGCAGA TCCTGGACCT CAGCAAAAGG TACATCAAGG CACTGGCAGA AGAAAACAGG
  361 AATGTGGTGG ATGGGCCATA CGCTGGAGTC ATGACAGCTT ATGATCTGAA GAAAACCCTT
  421 GCTGTGTTAT TAGATAACAT TTTGCAGCGC ATTGGCAAGT TGGAGTCGAA GGTGGACAAT
  481 CTTGTTGTCA ATGGCACCGG AACAAACTCA ACCAACTCCA CTACAGCTGT TCCCAGCTTG
  541 GTTGCACTTG AGAAAATTAA TGTGGCAGAT ATCATTAACG GAGCTCAAGA AAAATGTGTA
  601 TTGCCTCCTA TGGACGGCTA CCCTCACTGT GAGGGAAAGA TCAAGTGGAT GAAAGACATG
  661 TGGCGTTCAG ATCCCTGCTA CGCAGACTAT GGAGTGGATG GATCCACCTG CTCTTTTTTT
  721 ATTTACCTCA GTGANGTTGA AAATTGGTGT CCTCATTTAC CTTGGANNNC NAAAAATCCC
  781 TACGAAGAAN CTGATCATAA TTCATTGGCG GAAATTCNNA CAGNNTTTTA ATANTCTCTA
  841 CAGNATGNNG NAAAANCNTG NANNNANNNC GGNNGNNNNN NNNNANNNNN NCNGNNNNNA
901 NNNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA,6xHis,myc,insert 3'
Sequence file: RDB13428_A6Dqb.seq check
>13428_13428_B2EgF_3_BGH_rev2_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNNN GGGGGNNGGG CANACNACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACT CAATGGTGAT GGTGATGATG ACCGGTATGC
  121 ATATTCAGAT CCTCTTCTGA GATGAGTTTT TGTTCGAATG GGTGACCTCG AGCGGCCGCC
  181 ACTGTGCTGG ATAGGCAGTC TTTGCAGAGA GCCACCTGGC CCTTGATGAA GTCCCGGCAG
  241 GGGCAGACCC TCTGGTGCCT GGGGTGGGCG CCTGCACAGC TGAAGAGCAG GAGGTCACCT
  301 TGAAACACAC AGTGCTTATT CTTAGGGTCA AAGGAGGGCA CCAGGATGTC CTTGGCCAGC
  361 TCTGAGCTTT GGCAGGTCAC CTTGTACTTC AGCATGTCCT TGTCCTTGTT GAGGTGCTGG
  421 AAGAAAGAAG GCTCGCAGAT GAGCTGGCTC TCCTGGCACA CCTGCTTGCA GGACTGCCCG
  481 GGCTCAGCAA GCTTGACCTG TAGGGCGCTG AGGGGTGGCC ACATCACTTG CCCATGGCAG
  541 AAGTCCTGTT TTTCAATGAA AGCATTGATT CTCTGTAGCA TCCCCTCGCA CGTAAATTCA
  601 TATGGCATGT ATGGCTCAAT CTTCTGATTT AAAATTGCTT TCACTGCATC CTCTACTTCC
  661 TCCTGATTGT TGAGGTCAAC AGTCCACACA TGTGGCCGCC CGATGAAAAC TTCAGCGTAA
  721 GGATGCTGGG ATGTCAGCTC TCTCAGAGTT GGCTTGCCAA TGAAAAAGTC TGTGTTTTTG
  781 CTGCTTTTGG GTGGGTTGAA CTTGGGANTC AGAAAAGCAC ATCNNTTTGC GATAGCTTCC
  841 ANNGGAGCTG GGCCCTCGTA ANGNAACCCN AGTCCNACAA ACAACTTGGT TTCTCGAANA
  901 ANGAACTGCA GGNCCCGTCN ACNGNNNANA NNNNGGTTTT TCNCGNNNCT GGNNATANTC
  961 TTTGTGCTGN ANCCNTAAAC NNNNGCATGC NNNTNCANGN ATGNGNGNNT NANGNCCNAN
1021 NANNNNNNCN TNANNNNNNN NNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB13428_A6Dqc.seq check
>D02340A2_A6Dq_3_SV40pro_F_G05_20.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGNNGN NGCTTTTTTG GNNGCCTANG CTTTTGCNAA
   61 AAGCTCCCGG GNNCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT
  781 CNNGNCATAG CGTTGGCTAC NCGNGATATT GCTGAAGANC TTGGCGGCNA ATGGGCTGAN
  841 CGCTNCCNCN NGCNTTACNG NANCGCCGCN CCCGANNCNC ANCNNNNCGC CNTCNATCGN
901 NNTCTTGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Korekane, H., Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). J. Biol. Chem. 288 (39): 27912-27926 (2013). PMID 23960081. [link to RRC of NBRP]