RDB13513 - pGEMHE-mBubR1-WT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGEMHE-mBubR1-WT (#RDB13513)

Plasmid for in vitro transcription of mouse Bub1b in mouse oocyte.

Alternative name	pGEMHE-mEGFP-BubR1
Clone info.	Plasmid for in vitro transcription of mouse Bub1b tagged with mEGFP in mouse oocyte.
Comment	pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone	pGEMHE (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	mouse Bub1b cDNA Xenopus laevis beta-globin cDNA
Depositor\|Developer	Kitajima, Tomoya \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13513	pGEMHE-mBubR1-WT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEMHE-mBubR1-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13513).

Reference section:

Yoshida, S., Kaido, M., Kitajima, T.S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13513_A6Aep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB13513_A5Isa.seq
>D02675A5-A5Is_4_T7_20mer_B02_05.ab1 1 TACCCCGGTA CCAGCTTGCT TGTTCTTTTT GCAGAAGCTC AGAATAAACG CTCAACTTTG 61 GCAGATCAAT TCCCCGGTGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA 121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT 241 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA 301 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 541 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 601 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC 721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTTAAGGAGT TCGTGACCGC 781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAC TCAGATCTCG AGCTCAAGCT 841 TCGAATTCTG CAGTCGACGA TGGCGGAGGC GAGTGAAGCC ATGTGCCTGG AGGGAGCAGA 901 GTGGGAGCTG AGTAAAGAAA ACATACAGCC CTTTACGGCA CGGGCGGGTC ATGTCCACAC 961 TTCAGGAGCT TTGGGCAAGC AGAGTCAGCC CGGCCACACT GCTCTGCAGC AGCAGAAACC 1021 GGGCAATTTT GATCTGAAAT CCGCTTTTAC TCTGAGATGA ACCCTCTGGT ATGTGGACCG 1081 ATATATATGG GACAGAAACG GAACTTAA //
Primer: mEGFP-down2 Sequence file: RDB13513_A5Isb.seq
>D02675A5_A5Is_4_mEGFP-down2_E12_15.ab1 1 CTGAATTTGC AGTCGACGAT GGCGGAGGCG AGTGAAGCCA TGTGCCTGGA GGGAGCAGAG 61 TGGGAGCTGA GTAAAGAAAA CATACAGCCC TTACGGCACG GGCGGGTCAT GTCCACACTT 121 CAGGGAGCTT TGGCAAAGCA AGAGTCAGCC GGCCACACTG CTCTGCAGCA GCAGAAACGG 181 GCATTTGAAT CTGAAATCCG CTTTTACTCT GGAGATGACC CTCTGGATGT GTGGGACAGA 241 TATATTAATT GGACAGAACA GAACTACCCT CAAGGGGGGA AAGAGAGTAA CATGTCAGCG 301 TTAGTGGAGA GAGCGATAGA AGCACTCCAA GGAGAGACGC GCTATTATAA TGACCCCCGC 361 TTTCTCAGTC TCTGGATCAA ATTGGGACAT TTGTGCAATG AACCTTTGGA TATGTACAGC 421 TATTTACAAA GCCAAGGAAT TGGCGTTTCC CTTGCCCAGT TCTATATTTC ATGGGCTGAA 481 GAATACGAAG CTAGAGAAAA TTTCAAGAAA GCGGACATAA TATTCCAGGA AGGGATTGAA 541 CGCAAGGCTG AGCCCCTGGA CAGACTGCAG TCCCAGCACA GACAGTTCCA GTCTCGAGTG 601 TCGCGACAAG CTTTCTTGGC CCTTGGGAAT GAAGAGGAGA GCTTTGGAGC CTTCTGAACC 661 ACAGAGAGCT CGCTAGCTGA GCTGAAGCAA AGGAAGAAGA TGGCCAGAGC GCCATCAGCG 721 TGTCGAGGTG CTCTGAAAGC TCCAGTCGAG CGAAGGTCTA AATGCAGTTC CCCAGCAGTA 781 CACGGTAATC GCAGATCACC GTTTTGATGA ATGGCGAAAC CCGGCTCTAA CGATTATAGC 841 CTTGTACCCA CCATGGATGC ACCCTTGTGC CAGGCAAGGA AAGACTCACA GTCTAGGGAA 901 CAATGGCCGG GAACAGCCTA GAATCAGCCT GGGAGCGAAT CCAGGTCCAG TACGATGAAG 961 GCCCCAACTG AACCTGATGC TATACCGTTC CCGACCGGGA CGACCTTGGA GTCACTCCGT 1021 GAGGAGTCGG AAACT //
Primer: RDB13509#1 Sequence file: RDB13513_A5Isc.seq
>D02675A5-A5Is_4_RDB13509#1_D02_11.ab1 1 CGGGCCTTTT GTAGTGTAAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT 61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTAGGCA 121 GGGCTCTGGG AGCCGCCTGG CTGCTTGTCT TGCTGAGTGA GCAAAGCTTC CGGGCTGATT 181 GTCTTCCCCA GCTTCCACAG GGCTCTGTTC AGGTGGCTAT GGAATGTGGC ATCAAAAGCC 241 CCACCCATTT CTGCTGCCAG CTCCCCCAGA ACAGACACTG TGGACTTGTC ACTGGCATTC 301 AGAATCCGCA CAAAGAATTT ATTCCACAAT TCACTGTCTT TTAGCTCAGA GGTGCTCTGG 361 CTAAGTTTCC AGAGGAGTCC ATCCCAGAAG ACATGGAGGT GCTCCTTGAA CAGGAGTAAG 421 TGCGCTAGGT CTGCTATACC CAACAGATCT ACATGGTAGG GAGAAGAACA GTTAGCCAGG 481 ATCTTTTGTC CTTCCAGGAT CTGTGCAGTC CGAAAGCCAC TATAGGCAAA CGCATCCAGC 541 TGCACCCTCA GGTCAACACT GTAGGAGAAG TCCATGATCC TCACAGCGTG ATCGTCCTTA 601 TTTACATAGT CATAGGGGTC GTGGATTCTG TTTCGTAGGA TCAGACTCCG TGGACTCAAG 661 TCTCCGTGTA CTATTTCAGC TCTGTGTAGC TTCTCCACGA TTGTCAAGAG GTTGTAAATA 721 ATCAACACTA TTATTTCATG AGTAACAAAT TCGCTGTGTT GGAGAAGATT CTGAAGGGTG 781 GAGCAGTTTA TATACTGGTA CCAAACAACA TGGCCATCTT GGTACTGACA GCAGCTGCAA 841 AGCTGGTCAT AGTCCTCATT CAGACGCTCC TTCAACTTGA GGTTGATATA AAAAATCCCA 901 CGGGATAGGC TGAGATGATG CCTTTATCAT GGGTTAGCTC TGCAGAGCTT CTTTGGTGCC 961 ACCCCAGAAT AACCCTGTAA TCGTCACATG TTTAGTGCTC TTGCTTGATG ACGTTAATCT 1021 CAGACCCTAA CTCAATCTCG ACCCTATTTC AAGCACAGCA TCGGTTCGGA TCATCCACAG 1081 AAAACTTTCG CAAAAGTCAA CT //
Primer: RDB13513#1 Sequence file: RDB13513_A5Isd.seq
>D02675A5-A5Is_4_RDB13513#1_E02_14.ab1 1 CCGTTACCGA GAGGGAATGT TTGCAGGAAA GAGGATCTGG ATGGGAAAGG CCGGCCGAGG 61 CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA ATTATTGAAG 121 AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA GCTCCCAGTA 181 CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT GACGGGGCAG 241 AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA AAATCCCTAC 301 TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT GTGCTGGAAA 361 TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG CACCTAACAT 421 GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA ACCATGATAA 481 AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG AAGGAGCGTC 541 TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC CATGTTGTTT 601 GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC GAATTTGTTA 661 CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG AAGCTACACA 721 GAGCTGAAAA TAGTACACGG AGACTTGAGT CCACGGAGTC TGATCCTACG AAACAGAATC 781 CACGACCCCT ATGACTATGT AAATAAAGGA CGATCACGCT GTGAGGATCA TGGACTTCTC 841 CTACAGTGTT GACCCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC TTTCGGACTT 901 GCACAGATCC TGGAAAGGAC AAAAGATCCC TGGCTAACTG TTCTTCCTCC CTACCAATGT 961 AGATCTGTTT GGGGTATAGC CAGACCCTAG CCGCAACTTA CTTCCCTGTT CCAAGGGAGC 1021 CACCCTTCCA TGTTCTTTCC TTGGGAATGG ACCTTCCTTC TGGGAAACCT TTAGCCCAGA 1081 AGCCAACCCT TCTGAAGCTT AAAAAGACAC AGTTGAAAAT TTGTGGGAAT TAAATTCCTT 1141 TTGTTGCCGA TATTCTGAAA TGACCAAGGT GGGAAACAAG AAGATTTTCC CA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]