Please review the QC test results indicated by icon below as well as clone information before placing your order.

pLEX_ALDH2WT

Lentiviral plasmid vector of FLAG-tagged ALDH2

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13741_A6A5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13741_A6A5a.seq
>D02971A2_A6A5_1__CMV_Forward_E05_14.ab1     1 CTTAATCACC TCCGGCCCCA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT   121 TTTGACCTCC ATAGAAGACA CCGACTCTAC TAGAGGATCC ACTAGCGATT ACGCCAAGCT   181 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCCA   241 CCATGGATTA CAAGGATGAC GACGATAAGA GCCCGGGCGG ATCCCCCGGG CTGCAGGAAT   301 TCATGTTGCG CGCTGCCGCC CGCTTCGGGC CCCGCCTGGG CCGCCGCCTC TTGTCAGCCG   361 CCGCCACCCA GGCCGTGCCT GCCCCCAACC AGCAGCCCGA GGTCTTCTGC AACCAGATTT   421 TCATAAACAA TGAATGGCAC GATGCCGTCA GCAGGAAAAC ATTCCCCACC GTCAATCCGT   481 CCACTGGAGA GGTCATCTGT CAGGTAGCTG AAGGGGACAA GGAAGATGTG GACAAGGCAG   541 TGAAGGCCGC CCGGGCCGCC TTCCAGCTGG GCTCACCTTG GCGCCGCATG GACGCATCAC   601 ACAGGGGCCG GCTGCTGAAC CGCCTGGCCG ATCTGATCGA GCGGGACCGG ACCTACCTGG   661 CGGCCTTGGA GACCCTGGAC AATGGCAAGC CCTATGTCAT CTCCTACCTG GTGGATTTGG   721 ACATGGTCCT CAAAATGTCT CCGGTATTAT GCCGGCTGGG CTGATAAAGT ACCACGGGGA   781 AAACCATCCC CATTGACGGA GACTTCTTCA GCTACACACG CCATGAACCC TGTGGGGGTG   841 TGCGGGCAGA TCATTCCGTG GAATTTCCCG CTCCTGATGC AAGCATGGGA AGCTGGGGCC   901 CAGCCCTTGG CAACTGGAAA CGTGGGTTGT GATGAAAGGT AGCTGGAGCA GACACCCCTT   961 CACGGCCCTC TTATGTTGGC CAACCTGGAT CAAGGGAGCT GGGCTTTCCC CCCTTGTGGT  1021 TGGTTCAAAC ATTGGTGGCC TGGGATTGGG CCCACGGCTT GGGGCCGCAA TTGCCTTCCA  1081 TGGGAGCATG TGGACAAAGG TGCAATCCAG CGTTCACTGA ATTGGCCCGG TATCAGTTGA 1141 CTGTCGCTGG GGAGCA //
Primer: IRES-R Sequence file: RDB13741_A6A5b.seq
>D02971A2_A6A5_1__IRES_R_F05_17.ab1     1 CTTCCGCCGG GGCCTTCCGG GCAGGTAACG GTTAGGGGGG AGGGGAGGGG AGAGGGGGCG    61 ACCGGTGGCC AGACGCGTCG GGCCCTCTAG ACTCGAGTTA TGAGTTCTTC TGAGGCACTT   121 TGACTGTGAC AGTTTTCACT TCAGTGTATG CCTGCAGCCC GTACTCGCCC AACTCCCGGC   181 CACTCCCCGA CATCTTGTAG CCACCAAAGG GTGACTGGGC TCCAAACACA TCATAGCAGT   241 TGACCCACAC AGTGCCCGCC TGGAGGGCCT GGGACAGGTA ATTGGCCTTG TCCAAATCCT   301 TTGTGAAGAC AGCTGCGGCC AGCCCGTACG TGGAATTGTT GGCTCTCCCA ACAACCTCCT   361 CTATGGTCTT GAACTTCAGG ATCTGCATCA CTGGCCCGAA GATCTCCTCC TTGGCGATGG   421 TCATGCCATC CTGCACATCT CCAAACACAG TGGGCTGGAT GAAGTAACCA CGGTCAGCAG   481 CAATGCCCCC ACCACACAGC AGCTTCGCCC CCTCTTGCTT CCCCGTGTTG ATGTAGCCGA   541 GGATCTTCTT AAACTGAGTT TCATCCACCT GCGGCCCCTG CTCGGTCTTG CTATCAAAGG   601 GGTTCCCGAC CACCCGAGAC TTGGCCCGGG CAACGCTCCG CTCCACAAAC TCATCATAGA   661 TGTCCTCCTG CACGAAGGTC CGGGAGCCGG CACAGCAGCA CTGGCCCTGG TTGAAAGAAC   721 AGGGCGAAGT GGGCCTGTTC CACGGCCCAA TCCATATCGG CATCTGACAT GATGATGTTG   781 GGGCTCTTCC CCCCCCAGCT CCAAGGTCAC TCTCTTGAGG TTGCTGCTCC CAGCAGCAAC   841 CTGGATTACG CGGCCAATCT CAGTGGAGCC TGTGAATGCC ACTTTTGTCC CACATCCTTC   901 ATGGGGAAGG CAATGGCGGG CCCCAGCACG TGGGGCCAAA ATCCCAGGCC ACAATGTTGA   961 CCACACCCAG GGGGGAAAGC CAGCCCTCCT TGATCAGGGT TGGGCCACAT AGAAGGGCGG  1021 GTGAGGGGTG GTCTGCCTCA GCTAACCTTT CATTCACAAA CCACCGTTTT CCAAGTTTGC  1081 CCAAGGCTGG AGCCCAAGCT TTCCATGGCC TTGCCATCGA GAAGCGGGAA AATTTCCAAC 1141 GGGAAATGGA ATGCTTGCCC CGGCAACATC CCCCCCTACT ACAAGTGTTC TCATTGGT //
Primer: CMV_en_R Sequence file: RDB13741_A6A5c.seq
>D02971A2_A6A5_1_CMV_en_R_A10_01_ABI08.ab1     1 TCCGGTATAT GATTACTATT AATAACTAAT GCATGGCGGT AATACGTCTA GAACCTCGGA    61 CCTGCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC   121 TCACTATAGG AGGGCCACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG   181 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT   241 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA   301 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT   361 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT   421 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA   481 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGATTCCGG ACTAGAGCGG GCCCGGTACT   541 AACCAAACTG GATCTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT TTTTGTAATT   601 TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT TCTTTCCCCT   661 GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC TGCCATTTGT   721 CTGCAGAATT GGCGCACGCA GTGCCGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC   781 TCTCTCTCCA CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG   841 GTGGGTCTGA AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC   901 AGCAAAAACT ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA   961 CCACAGCCAA TTTGTTATGT TAAAACCAAT TCCACAAACT TGCCCATTTA TCTAAATTCC  1021 CAATAAATTT CTTGTTCATT CTTTTCTTGC TGGGTTTTGC GAATTCTTTC CATTTAAAGA 1081 GTGTATAAGG CCTGCTGTAT GGTAATTTCT CTGATCCCCA CCTCCAATTC CCCAGGGA //
Primer: IRES-F Sequence file: RDB13741_A6A5d.seq
>D02971A2_A6A5_1__IRES_F_G05_20.ab1     1 CAAATCCTTT AAATGTTGTT TAGTCGAGGT TAAAAAAAAC GTCTAGGCCC CCCGAACCAC    61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG GCCACCGAGT ACAAGCCCAC   121 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT   181 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC   241 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC   301 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT   361 GTTCGCCGAG ATCGGCTCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA   421 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC   481 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG   541 AGTGGAGGCG GCCGAGCGCG CTGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA   601 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG   661 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTTAACAATC AACCTCTGGA   721 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGGCTAT   781 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT   841 TCTCCTCCTT GTATAAATCC TGGGTTGCTG TCTCTTTATG AGGAGTTTGT GGGCCCGTTT   901 GTCAGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCCAC TGGTTGGGGG   961 CATTTGCCAC CAACCTGTCA GCTCCTTTCC CGGGACTTTC GCTTTCCCCC CTCCCTATTG  1021 CCACGGCGGA AACTCATCGC CGCTTGCCTT GCCGCTGCTG GACAAGGGGC CTCGGCCTGT  1081 TGGGCCACTG ACAATTTCCG TGGGTGTTGG TCGGGGGAAA GCCTGAACGT TCCTTTACAA 1141 TGGCTGGCTT CGCCTGTTGA TTGTCCACCG TGAAATTTCT GTGCGCCG //
Primer: HIVgp_R1 Sequence file: RDB13741_A6A5e.seq
>D02971A2_A6A5_1__HIVgP_R1_H05_23.ab1     1 TCGGCTTCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG   841 GTCTAACCAG AGAGACCCAG TACAGGCAAA AAGCAGCTGC TTATATGCAG GATCTGAGGG   901 CTCGCCACTC CCCAGTCCCG CCCAGGCCAC GCCTCCCTGG GAAAGTCCCC AGCGGAAAGT   961 CCCTTGTAGC AAGCTCGATA TCAGCAGTTC TTGAAGTACT CCGGGATGCA GCTCTCGGGC  1021 CACGTGATGA AATGCTAGGC GGGCTGTCAA ACCTCACTCT AACACTTCTT CTTCTCCGGG  1081 GTCATCATCC CATGCAGCTC CACAGGTGGT TAACAAGGCT GGTGTCTCCT CCATTTATTG 1141 GCTCTTCTAA CTTATCTTGG CTCACTGTAC ATAGCCTGTA AGCCACTCTA ACT //
Primer: BGH_rev Sequence file: RDB13741_A6A5f.seq
>D02971A2_A6A5_1_BGH_rev_A06_03.ab1     1 AGGTTTTAAA GGGTCAAGGC CTTCTCACTC TCTGATATTC ATTTCTTTGC AAGTTATAAA    61 TACTGAATAA TAAGATGACA TGAACTACTA CTGCTAGAGA TTTTCCACAC TGACTAAAAG   121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAAG CAAAAAGCAG ATCTTGTCTT   301 CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CTCTTTTCTT TTAAAAAGTG GCTAAGATCT   361 ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG AAAACCCACC   421 TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC TGTATTGCTA   481 CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACTGCAGA ATTAATTCCA GGCGGGGAGG   541 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA   601 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC   661 AGAAGGACGT CCCGCGCAGA ATCCAGGGTG GCAAACACAG GCGAGCAGCC ATGGAAAGGA   721 CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AAACAGCCGA GCCCCTGTCC   781 AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGGAAAGCG   841 AAAGTCCCGG AAAAGGGAGC TGACAGGTGG TGGCAATGCC CCAACCAGTG GGGGTTGCGG   901 TCAGCAAAAC ACAGTGCACA CCACGCCACG TTGCCCTGAC AAACGGGGCC ACAACTCCTC   961 ATTAAAGGAG GACAGCAACC AAGGATTTAT ACAAGGGAAG GAGGAAAAAT GAAAGCCAAT  1021 ACGGGGAAGC AATAGCAATG GATACAAAGG GCATTTAAAA GCAGCCGTTA TTCCCACATA  1081 GCGTAAAAGG GAGGCAAACA TTAGTTAGAG GAAATACCAA GTTCAATCTT TTTCAACAAA  1141 ATTTTTGGTT AATTCCCAGG AAGGGTTTGA TTGTTACTTC AGGCACCCGG GGCTTGCGAG 1201 GGTTCACATG GCACCTAGGG GTGTCGCCGC AGGT //
Primer: SV40pro_F Sequence file: RDB13741_A6A5g.seq
>D02971A2_A6A5_1_SV40pro_F_B10_02_ABI08.ab1     1 CCTTGTAACA TTCAGGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG    61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGATGAA AAAGCCTGAA   121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG   181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA   241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG   301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG   361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA   421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC   481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT   541 ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG   601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC   661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG   721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC   781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG   841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT   901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG GTTGACGGCA ATTTCGATGA   961 TGCAGCTTGG CGCAGGGTCG ATGCGACGCA AATCGTCCGA TCCGGAGCCG GGACTGTCGG  1021 GCGTACACAA TCGGCCCGCA GAGCGCGGCC GTCTGGGACC GGAATGACTG TGTAGAAGTA  1081 CTCGCCGATA GTGAACGGAA CGCCCTAGCA CTCGGTCCGG AGGGGCCAAA AAGGCAAATA 1141 AGTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl