A donor vector for introducing CMV-OsTIR1 at the human AAVS1 locus.
|
Other clones in our bank
External Database human AAVS1 Oryza sativa OsTIR1 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
---|---|
Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
---|---|
提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB13918 | pMK232 (AAVS1 CMV-OsTIR1-PURO) | DNA solution |
Materials & Methods section:
The pMK232 (AAVS1 CMV-OsTIR1-PURO) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13918). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
---|---|
Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13918_A6B8p1-4.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: M13R (Pr0160) Region: insert mid(AAVS1 arm1 mid) Sequence file: RDB13918_A6B8o.seq |
---|
>D03070A02_A6B8_1_M13R_A10_01.ab1 1 NNNNNNNNNN CNNNNNNAAN GGGNNTAGTC CTGCAGGTTT AAACGAATTC GCCCTTTGCT 61 TTCTCTGNCN AGCATTCTCT CCCCTGGGCC TGTGCCGCTT TCTGTCTGCA GCTTGTGGCC 121 TGGGTCACCT CTACGGCTGG CCCAGATCCT TCCCTGCCGC CTCCTTCAGG TTCCGTCTTC 181 CTCCACTCCC TCTTCCCCTT GCTCTCTGCT GTGTTGCTGC CCAAGGATGC TCTTTCCGGA 241 GCACTTCCTT CTCGGCGCTG CACCACGTGA TGTCCTCTGA GCGGATCCTC CCCGTGTCTG 301 GGTCCTCTCC GGGCATCTCT CCTCCCTCAC CCAACCCCAT GCCGTCTTCA CTCGCTGGGT 361 TCCCTTTTCC TTCTCCTTCT GGGGCCTGTG CCATCTCTCG TTTCTTANGA TGGCCTTCTC 421 CGACNGANGT CTCCCTTGCG TCCCGCCTCC CCTTCNTGTN NNNCTGCATC ATCACCGTTT 481 TTCTGGACAA NCCCNAAGTA CCCCGTCTNC CNGGCTTTAN CCACCTCTNC ATNNNCTTGN 541 TTTCTTNGCC NNNNNNCCCC NNNCTNNNGN GNANTNNNNT CANNNNNNAN TNNNTTNNNT 601 NNGGNANCTN CCCNANCCCC NNNNNNNNNN // |
Primer: M13_-40 (Pr0410) Region: T7 pro,insert 3'(AAVS1 arm2 3') Sequence file: RDB13918_A6B8p.seq |
>D03070A02_A6B8_1_M13(-40)_B10_04.ab1 1 NNNNNNNNNN NANNNGNNTN NNNNTAGGGC GAATTGAATT TAGCGGCCGC GAATTCGCCC 61 TTAGAGCAGA GCCAGGAACC CCTGTAGGGA AGGGGCAGGA GAGCCAGGGG CATGAGATGG 121 TGGACGAGGA AGGGGGACAG GGAAGCCTGA GCGCCTCTCC TGGGCTTGCC AAGGACTCAA 181 ACCCAGAAGC CCAGAGCAGG GCCTTAGGGA AGCGGGACCC TGCTCTGGGC GGAGGAATAT 241 GTCCCAGATA GCACTGGGGA CTCTTTAAGG AAAGAAGGAT GGAGAAAGAG AAAGGGAGTA 301 GAGGCGGCCA CGACCTGGTG AACACCTAGG ACGCACCATT CTCACAAAGG GAGTTTTCCA 361 CACGGACACC CCCCTCCTCA CCACAGCCCT GCCAGGACGG GGCTGGCTAC TGGCCTTATC 421 TCACAGGTAA AACTGACGCA CGGAGGAACA ATATAAATTG GGGACTAGAA AGGTGAAGAG 481 CCAAAGTTAG AACTCAGGAC CAACTTATTC TGATTTTGTT TTTCCNAACT GCTTCTCCTC 541 TTGGGAAGTG TAAGGAAGCT GCAGCACCAG GATCAGTGAA ACGCACCAGA CAGCCGCGTC 601 AGANCAGCTC ANGTTCTGGG AGAGGGTAGC GCANGGTGGC CACTGAGAAC CGGGCANGTC 661 ACGCATCCCC CCCTTCCCTC CCACCCCCTG CCNAGCTCTC CCTCCCANGN TCCTCTCTGG 721 CTCCNTCGTA AGCAAACCTT ANAGGNTCTG GCAAGGAGAG AGANGGNTCC AGGAAATGGG 781 GGGTGTGTCA CCAGATANNN AATCTGCCNN ACNNNANNTG GGGGTTAGAN CCNANATCNN 841 NNANTAGNNA NGNNNNAGNN CNNANNNNNG GGCTTTNNNG TCNNNNNTNC TGNNCCTANN 901 NANCNNNANA NCCNANNGNN NNCCNNNNNN NNNN // |
Primer: CMV_Forward (Pr0016) Region: insert mid(CMV pro,OsTIR1 5') Sequence file: RDB13918_A6B8q.seq |
>D03070A02_A6B8_1_CMV_Forward_D10_10.ab1 1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTG CCACCATGAC ATACTTTCCT 121 GAAGAGGTCG TCGAACACAT TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG 181 AGCCTGGTCT GCAAAGTGTG GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC 241 GGCAACTGCT ATGCTGTGAG AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA 301 CTGACCGTCA AGGGGAAACC CCACTTCGCC GACTTTNACC TGGTGCCCCC TGATTGGGGA 361 GGANACNCCN GNCCTTGGAT CGAGGNAGCC GCTCGCGGCT GTCNNGGACT GGANGAACTG 421 NNCNTGAANC GAANGGNGGN CTCTGANNAA AGTCNGGNNC TGCNNNNNNN NANCNNNCNN 481 NNGNTNNNNN NNNNGNNNNT GANTTNNNNN NNNANNNNNN CNNNNCNNNN NGNNNNNNNN 541 NN // |
Primer: SV40polyA_R2 (Pr0623) Region: insert mid(OsTIR1 3') Sequence file: RDB13918_A6B8r.seq |
>13918_13918_A9KrF_SV40polyA_R2_D09_12_ABI24.ab1 1 NNNNNNNNNN NGNANNNNNN GTTAANNANN ACNATTGCAT TCNTTTTATG TTTCNGGTTC 61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG 121 ATTATGATCT AGAGTCGCGG CCCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACGGGCCC 181 GGGATCCGAT GGATCCTCAC AGAATCTTCA CAAAGTTGGG AGCATCATCT CTTGCCCCTG 241 CGGTGGTCCG ATACACATAC AGTTTCTCCA CCTTTGGCAG GTCGCCATGA TTTTCCTCCA 301 TTTCGTTAGA CCCGTCCCGC TCATTAATGA CTTCCACGTT CAGCATAGGC ATTTTACTAG 361 CCAGGACCTG ACAGCCCTTC AGAGTCACAT TGCAACTAGA CATCCACAGG CTCCTCATTG 421 TCTCGTATCT GGCAAAGTTT CCCAGCAGAG CGGCATCCCC GAAAGGGCTG TCCCGGATTT 481 CCAGTTTTCG CAGATTCTTA CACCCGTTCA TCACGTGCAT CATGCCCTTA TCGGAGTCTC 541 CGGCAAATGC AATGCTCAGC ATCTCCAGCT GCTTAGCATA CTTGCCGATG TACATGAACA 601 CTTTGTCTGT CAGCAGGCCG GAAATGCTCA GCCTCTGGAG TCCCTTGCAC TCTCTCACGA 661 TAGCTCCAAA CCCTTCATCC AGTGGCTGGG ANGTGACCAC GTCGGGNTTG CCTGGTTCCA 721 GAATACACAG CCGAANTCGG GTGAAATTAG GGCAGTTCTT GGCGACNGTC ACCAGTGCAG 781 CATTAGTCAT CTGATGACAG AAATACAGCA NGNTGTTCNN CTTTGGGCAT CCCAGTGAGA 841 NTGNCACCAN GNNNTNCTCA NNGACNGCAC TATATNCNNN CNCNTAAAAA TCNNANNGGN 901 ANNNTCNCAN CTNCNNGANG NNCTNNNNNN ANCTNNNNAN NNCCNNGNNC CNNNNNNNNN 961 N // |
Primer: BGH_rev2 (Pr0606) Region: insert mid(bGH pA,PuroR,hPGK pro) Sequence file: RDB13918_A6B8s.seq |
>D03070A02_A6B8_1_BGH_rev2_E10_13.ab1 1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGGGTACC TCAGGCACCG GGCTTGCGGG TCATGCACCA GGTGCGCGGT CCTTCGGGCA 121 CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA GAAGGGGAGG TTGCGGGGCG 181 CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC CGCCTCCACT CCGGGGAGCA 241 CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA GACGCCGACG GTGGCCAGGA 301 ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC TTCCATCTGT TGCTGCGCGG 361 CCAGCCGGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT CTCGGCGAAC ACCGCCCCCG 421 CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC GCCGTCGTCC GCGACCCACA 481 CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC TTGCAGCTCG GTGACCCGCT 541 CGATGTGGCG GTCCGGATCG ACGGTGTGGC GCGTGGCGGG GTAGTCGGCG AACGCGGCGG 601 CGAGGGTGCG TACGGCCCTG GGGACGTCGT CGCGGGTGGC GAGGCGCACC GTGGGCTTGT 661 ACTCGGTCAT GGTAAGCTCC GGTGGATCCC CCTGGGGAGA GAGGTCGGTG ATTCGGTCAA 721 CGAGGGAGCC GACTGCCGAC GTGCGCTCCG GANGCTTGCA GANTGCGGAN NACCGCGCGG 781 GCANGAACAG GGCCCACACT ACCGCNNCNC ACCCCGCCTC CCGCACNGCC CCTTCCCGGC 841 CGCTGCTCTC GGCGCGNCCT GCTGANCAGC CGCTNTTGGC CNCAGCCNNT CNNNGNCGGC 901 GCGCTGCNNT NNCTNCCTNN CGCTGNNCGT CTGCGAGGGN NNNNNNNAGA CGTGNNNCTT 961 NCGNTTNNNN CNNNNNGGCA NGCCNCNNNN NGNNNGNNNN NNTNNCCNNN NNTNNNNNNN 1021 NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
---|---|
reference | Sathyan, K.M., An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion. Genes Dev. 33 (19-20): 1441-1455 (2019). PMID 31467088. |
reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2024.03.26
HTML_filter_RDB_240326.pl