Resource data sheet
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pCAG-EGxxFP-PK (#RDB13941)

A simple validation system for the mouse Kit WE genomic region targeted double strand breaks.

Clone info. A simple validation system for the mouse Kit WE genomic region targeted double strand breaks by observing GFP reconstituted.
Comment Mouse genomic region near the Kit gene (Chr. 5: 75,408,224–7,5408,767 of NC_000071.6). Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pCAG-EGxxFP (RDB13532) (Plasmid)
Size of vector backbone 6.9 kb
Selectable markers Amp^r
Gene/insert name mouse Kit genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Sci Rep. 5:13632, 2015.)
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep. 5:13632, 2015.)
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13941 pCAG-EGxxFP-PK Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCAG-EGxxFP-PK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13941).

Reference section:

Mizuno, S., Takami, K., Daitoku, Y., Tanimoto, Y., Dinh, T.T., Mizuno-Iijima, S., Hasegawa, Y., Takahashi, S., Sugiyama, F., Yagami, K., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Mizuno, S., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620. [link to RRC of NBRP]