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pcDNA3-FLAG-HERC2

Expression vector of FLAG-tagged human HERC2.

Catalog number RDB13979
Resource name pcDNA3-FLAG-HERC2
Clone info. Expression vector of FLAG-tagged human HERC2.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human HERC2 cDNA
Depositor|Developer Imai, Yuzuru |
Other clones in our bank
External Database
human HERC2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。
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          RDB13979 pcDNA3-FLAG-HERC2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3-FLAG-HERC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13979).

          Reference section:

          Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13979_A6C2p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5' (hHERC2)
          Sequence file: RDB13979_A6C2a.seq check
          >D03172A7_A6C2_3_CMV_Forward_E02_14.ab1
              1 ATTATCCAAT TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
            121 TACGACTCAC TATAGGGAGA CCCAAGCTTG GTACCGAGCT CGGATCTCCG CCATGGACTA
            181 CAAGGACGAC GATGACAAGC CGGCCGCGGA TCTGGAATTC CTCGAGATGC CCTCTGAATC
            241 TTTCTGTTTG GCTGCCCAGG CTCGCCTCGA CTCCAAATGG TTGAAAACAG ATATACAGCT
            301 TGCATTCACA AGAGATGGGC TCTGTGGTCT GTGGAATGAA ATGGTTAAAG ATGGAGAAAT
            361 TGTATACACT GGAACAGAAT CAACCCAGAA CGGAGAGCTC CCTCCTAGAA AAGATGATAG
            421 TGTCGAACCA AGTGGAACAA AGAAAGAAGA TCTGAATGAC AAAGAGAAAA AAGATGAAGA
            481 AGAAACTCCT GCACCTATAT ATAGGGCCAA GTCAATTCTG GACAGCTGGG TATGGGGCAA
            541 GCAACCAGAT GTGAATGAAC TGAAGGAGTG TCTTTCTGTG CTGGTTAAAG AGCAGCAGGC
            601 CCTGGCCGTC CAGTCAGCCA CCACCACCCT CTCAGCCCTG CGACTCAAGC AGAGGCTGGT
            661 GATCTTGGAG CGCTATTTCA TTGCCTTGAA TAGAACCGTT TTTCAGGAGA ATGTCAAAGT
            721 TAAGTGGAAA AGCAGCGGTA TTTCTCTGCC TCCTGTGGAC AAAAAAAGTT CCCGGCCTGC
            781 GGGCAAAGGT GTGGAGGGGC TCGCCAGAGT GGGATCCCGA GCGGCGCTGT CTTTTGCCTT
            841 TGCCTTCCTG CGCAGGGCCT GGCGATCAGG CGAGGATGCG GACCTCTGCA GTGAGCTGTT
            901 GCACGAGTCC CTGGACGCCC TGCGAGCACT TCCCGAGGCC CTCGCTCTTT GACGAGAGCA
            961 CCGTGTCCTC TGTGTGGCTG GAGGTGGTGG AGAGAGCGAC CCAGGTTTCC TCAGTCCCGT
           1021 CGTGACGGGG ATGGTTCACG GAAACGCCAG CACTAAGGAT CAAGAAGCAT CCCCCTTGCA
          1081 GGACAAGCAC TGGTCCTTGG CTATCTGCTG AACTGACTGG TGTGCCAG
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGHpA,insert 3' (hHERC2)
          Sequence file: RDB13979_A6C2b.seq check
          >D03172A7_A6C2_3_BGH_rev2_F02_17.ab1
              1 CCAATGCCGG GCTTAAGGGA GAGCACGGGG GGAGGGGCAA ACAAACAGAT GGCTGGCAAA
             61 CTAGAAGGCA CAGTCGAGGC TGATCAGCGA GCTCTAGCAT TTAGGTGACT ATAGAATAGG
            121 GCCCTCTAGA TGCATGCTCG ACGCGGCCGC TTAGTGTCCT GTTAAATAAT CTTGTGTAGA
            181 GTCCGAAGCA AAGGAGTCGA CATCCTCGTT ATCTGAATCG TCGCTGCTGT CGTCGGCGGC
            241 TGGCTCTCCT GTAAGTGCGA TGCGAGCGTA GTCATCTGTG TCTATGGACT TGCAGAAGTG
            301 GATGGCGTAC TTGAGCTTCT CCTCCAGCAC CTGCTTGCAG GAATACCTGG GCAGCTTCAG
            361 CAAGAAGAAA CAGGTGTAGG ACTCAGGGAG GAAGTGGTCT GGAGGGTTGT ATTTATCCAA
            421 CACCTGGATG ACGAAGTCTC GGCCCCGGAA GTCGGCGATG GTCCTGGGCA GCCTCGTCCG
            481 GCCCCAGACG AAGCGAAGGA AAAGAGAGCG CTCTGTGTTG GAGAAGGACT CCATCACCTC
            541 CCAGAACCAC TGGATCAGCG ATGCGGAAGG CTCGATGCCT TTATAGGTGG CCACCGACTT
            601 GAGAAGGTGC AGCGGGATGT CAGGGCTGCC ACACACCATC GTCTCCAGTT CGTAGCCGGT
            661 GAACAGAGAG AGGGAGGGGA ACAGGCACAA CGCGGGCCAT TCCTTCCCGA ACAGCAGCCA
            721 CCTGCTCATC AAATTCATGG AGTCTATAGT TTATCGCCAG CCGCACGTAC TCCGCGCGGT
            781 TGTCCAGGGT GATGTGTGTG TGCTTGGAGC TCAACTGAAT GTCCTGGGCC ACTGGCACTT
            841 GGGCACTGTG AAAGGGCAGG CTCATGGCTT TCAAAACTCC TCTGAGGGTG GGCTTCATTG
            901 TCTCGGGATG TACATGAAGT CCAGGAAATA AAAATCCCTT ATCAACCCTC ACTGAAGGTC
            961 CGGCGATGGG TGAAGGCTCA TCCCAAGCCA GCCTGCTTTC AAGACAGGGC CTCGGGCAAG
           1021 GTTGGAGGCT CAAGGGGGAC TTCCCGGTTC GGAATGGCAA TGCCCAGCAA CCACACCCCA
           1081 GAAGCGGGAC AATGCTGGCT GGGTGCCACG GGTGGCTCTG GCCGGTCCGG GCCTTGAGAC
          1141 CAGGGTAGAC CAAGTTCTTT CG
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB13979_A6C2c.seq check
          >D03172A7_A6C2_3_SV40pro_F_G02_20.ab1
              1 GCCCGGTCCC TTGACTTTCC AGAATAGTGG AGGGAGGCTT TTTTGGAGGG CCTAAGGCTT
             61 TTGCAAAAAG CTCCCGGGAG GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT
            121 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
            181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
            241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
            301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
            361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
            421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
            481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
            541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
            601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
            661 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
            721 TCATGGTGGA AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
            781 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
            841 TGGGGCTGAC CGCTTCCCTC GGTGCTTTAC GGGTATCGCC GCTCCCGGAT TCGCAGCGGC
            901 ATCGCCTTCT ATCGCCCTTC TTGACGAAGT TCTTTCTGAG CGGGGACTCC TGGGGGTTCG
            961 AAAATGACCG ACCCAAGCCG AACGCCCAAC CCTGCCCAAT CACGAGATTT CGGATTTCCA
           1021 ACCCGGCCCG GCCTTCTAAT GAAAGGGTTG GGGCCTTCCG GGAAATCGTT TTTTCCGGGG
           1081 GAAGCCGGCC TGGGATGGAA TCCCTCCCAG CGCGGGGGAA TCCTCAATGC TGGGGAGCTT
          1141 ATTCGGCCTC ACACCCTCCA AACTTGTGTT CATAATGTGC GACAGC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl