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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-mMsh6 (#RDB14049)

Retroviral expression vector of mouse Msh6.

Alternative name clone 4
Clone info. Retroviral expression vector of mouse Msh6.
Comment Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Msh6 cDNA
Depositor|Developer Okazaki, Yasuji | Iseki, Hiroyoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14049 pMXs-mMsh6 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-mMsh6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14049).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14049_B0Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol, insert 5'
Sequence file: RDB14049_B0Fma.seq check
>D03255D4_B0Fm_1_MMLV-pol_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCGNANC TNGGNNACNC GCCGCCCNCG TGAAGGCTGC
   61 CGACCCCGGG GGTGGNNCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCA
  121 CCATGTCCCG ACAAAGCACC TTGTACAGCT TCTTCCCCAA GTCCCCGGCA CTCGGCGACA
  181 CCAAGAAGGC CGCGGCCGAG GCCTCACGCC AAGGTGCCGC TGCCTCTGGA GCCTCCGCTT
  241 CCCGCGGCGG GGATGCGGCC TGGAGCGAGG CTGAACCCGG GTCCCGGTCC GCGGCGGTAT
  301 CCGCCTCGTC GCCGGAGGCA AAGGATCTCA ACGGAGGGCT GCGGAGGGCC TCCTCCTCCG
  361 CGCAGGCCGT GCCTCCCAGT TCTTGTGACT TCTCACCAGG TGATTTGGTT TGGGCTAAGA
  421 TGGAAGGTTA CCCCTGGTGG CCTTGCCTAG TTTATAATCA TCCCTTTGAT GGAACGTTCA
  481 TCCGGAAGAA AGGGAAATCT GTCCGTGTTC ATGTACAGTT CTTTGATGAC AGCCCAACAA
  541 GGGGCTGGGT TAGCAAAAGG ATGTTAAAGC CATATACAGG TTCAAAGTCA AAGGAAGCCC
  601 AAAAGGGAGG TCATTTCTAC AGTTCAAAGT CTGAGATACT CAGAGCAATG CAACGTGCAG
  661 ATGAGGCCTT AAGTAAAGAC ACGGCTGANA NGCTGCAGCT GGCAGTGTGT GATGAGCCTT
  721 CAGAGCCGGA NGANGANGAN GANACAGANG TGCATGANGC TTATTTATCT GATAAGAGTG
  781 AAGAGGANAA TTACAACGAG AGTGAAGAAG ANNCACAGCC CAGNGTTCNG GGGCCTANGC
  841 GANNCAGCNN GCAAGTCNAG AANNCGACGG GNCATCTCAN ANTCNGANAN TGANNNNNGN
  901 GGNTCTGATG NANANNTCNN NCCNNANNCT AANNNNNNNN NANCNNNGAT GNNNCNNNCN
961 NNNNNNTTNG GGNNNNNCNN NNNNNNNN
//
Primer: RDB14049#1 (Pr0866)
Region: insert 3', 3' LTR
Sequence file: RDB14049_B0Fmb.seq check
>D03255D4_B0Fm_1_RDB14049#1_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NCNNNNCNCN NTCNTTNNTT AGTAGAAGAN TATTCTAAAA
   61 GTGTTTGTGT GCGCCTAGGA CATATGGCAT GCATGGTAGA AAATGAATGT GAGGACCCCA
  121 GCCAGGAGAC TATTACTTTC CTCTATAAAT TCATTAAGGG AGCTTGTCCT AAGAGCTATG
  181 GCTTTAATGC AGCAAGGCTT GCTAATCTCC CGGAGGAGGT TATTCAGAAG GGACACAGAA
  241 AGGCAAGAGA ATTTGAGAGG ATGAATCAGT CATTACAGCT ATTTCGGGAA GTTTGTCTGG
  301 CTACTGAAAA GCCGACTATA AACGGTGAAG CTATCCATAG GCTGCTGGCC TTGATTAACG
  361 GATTGTAGTC GACGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA
  421 AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA
  481 AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG
  541 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA
  601 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  661 GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG TTTCTAGAGA
  721 ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC TTATTTGAAC
  781 TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCNNCGA NCTCAATAAA
  841 AGANCCCACA ACCCCNTCAC TCGGGGCGNN NGTCCNCCNA TTGANNNANT CGCCCNGGGN
  901 ANCCNNNNAT NCNANAAACC NNCTTGCANT NNCATNCNAN TTNNGGNNNC GCNNTTCCNN
961 NGNNNGNNNN NCNNNNNNNN NN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi, 5' LTR
Sequence file: RDB14049_B0Fmc.seq check
>D03255D4_B0Fm_1_gag-N_R_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA TTNNNNNACN AATACNGAAA CNCAGTCAGA
   61 CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT TCGGTCCCAA
  121 ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC TCCTACCAGA
  181 ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAGTC CAAAACGATC GGGATTTTTG
  241 GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGACGTCTCC CAGGGTTGCG
  301 GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA GCTAGTTAGC
  361 TAACTAGTAC CGACGCAGGC GCATAAAATC AGTCATAGAC ACTAGACAAT CGGACAGACA
  421 CAGATAAGTT GCTGGCCAGC TTACCTCCCG GTGGTGGGTC GGTGGTCCCT GGGCAGGGGT
  481 CTCCCGATCC CGGACGAGCC CCCAAATGAA AGACCCCCGC TGACGGGTAG TCAATCACTC
  541 AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG ATGCAACTGC AAGAGGGTTT
  601 ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG GACTGGCGCG CCGAGTGAGG
  661 GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC GCGAACAGAA GCGAGAAGCG
  721 AACTGATTGG TTAGTTCAAA TAANGCACAG GGTCATTTCA GGTCCTTGNG GCACCCTGGA
  781 AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC GCATCTGGGG ANCATCTGTT
  841 CTTGGCCNTN ANCCGGGGCN NGANCTGCTT ANNACAGATA TNCTGTTNGG NCNATATTCA
  901 NCTGTTNCNT CTGNTCTTGG NCCNNANCCG GGNNNNGNNN TGNTNANCNC ANATATCCNG
961 TTTNGNNNNN NNNTNNNNNN NTNNNNNNNN NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]