Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-TUBA1A flag-R64W

Expression vector of FLAG-tagged human TUBA1A mutant, R64W.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14141_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14141_A6Dpa.seq
>D03180A2_A6Dp_2_CMV-Forward_B05_05.ab1     1 ATTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGATTA CGCCAAGCTC GAAATTAACC   121 CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCCAT GCGTGAGTGC   181 ATCTCCATCC ACGTTGGCCA GGCTGGTGTC CAGATTGGCA ATGCCTGCTG GGAGCTCTAC   241 TGCCTGGAAC ACGGCATCCA GCCCGATGGC CAGATGCCAA GTGACAAGAC CATTGGGGGA   301 GGAGATGATT CCTTCAACAC CTTCTTCAGT GAGACGGGGG CTGGCAAGCA TGTGCCCTGG   361 GCAGTGTTTG TAGACTTGGA ACCCACAGTC ATTGATGAAG TTCGCACTGG CACCTACCGC   421 CAGCTCTTCC ACCCTGAGCA ACTTATCACA GGCAAGGAAG ATGCTGCCAA TAACTATGCC   481 CGAGGGCACT ACACCATTGG CAAGGAGATC ATTGACCTCG TGTTGGACCG AATTCGCAAG   541 CTGGCCGACC AGTGCACGGG TCTCCAGGGC TTCTTGGTTT TCCACAGCTT TGGTGGGGGA   601 ACTGGTTCTG GGTTCACCTC CCTGCTCATG GAACGTCTCT CAGTTGATTA TGGCAAGAAG   661 TCCAAGCTGG AGTTCTCTAT TTACCCGGCG CCCCAGGTTT CCACAGCTGT AGTTGAGCCC   721 TACAACTCCA TCCTCACCAC CCACACCACC CTGGAGCACT CTGATTGTGC CTTCATGGTA   781 GACAATGAGG CCATCTATGA CATCTGTCGT AGAAACCTCG ATATTGAGCG TCCAACCTAT   841 ACTAACCTGA ATAGGTTAAT AGGTCAAATT GTGTCCTCCA TCACTGCTTC CCTGAGATTT   901 GATGGGAGCC CTGAAATGTT GACCTGACAG AATTTCCAGA CCAAACCCTC TGCCCTATCC   961 CCCGCATCCA CTTTCCCTCT GGGCCACATA TGCCCCCTGT CATCTCCTGC TGGAGAAAGG  1021 CCTACCATGG AACCAGCTTT TCCTGGTAGC AGAAGATCAC GAATGCGTTG CATTGAAGTA 1081 GTCGAAGCCA AGAATTGGAT GA //
Primer: T7_20bp Sequence file: RDB14141_A6Dpb.seq
>D03180A2_A6Dp_2_T7_20bp_F05_17.ab1     1 TATTACTGGT ACTTATTAAT TAAGGTACCG GGCCCTACTT ATCGTCGTCA TCCTTGTAAT    61 CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGGTAT TCCTCTCCTT   121 CTTCCTCACC CTCTCCTTCA ACAGAATCCA CACCAACCTC CTCATAATCC TTCTCAAGGG   181 CAGCCATGTC CTCACGGGCC TCTGAAAACT CACCTTCCTC CATCCCCTCC CCAACGTACC   241 AGTGAACAAA GGCACGTTTG GCATACATCA GGTCAAACTT GTGGTCCAGG CGAGCCCAGG   301 CCTCAGCAAT GGCTGTGGTG TTGCTCAGCA TGCACACAGC TCTCTGTACC TTGGCCAGGT   361 CTCCACCAGG CACCACAGTG GGAGGCTGGT AGTTGATGCC AACCTTGAAG CCAGTGGGGC   421 ACCAATCCAC AAACTGGATG GTACGCTTGG TCTTGATGGT GGCAATGGCA GCATTGACAT   481 CTTTGGGAAC CACGTCACCA CGGTACAACA GGCAGCAAGC CATGTATTTA CCATGGCGAG   541 GGTCACATTT CACCATCTGG TTGGCTGGCT CAAAGCAAGC ATTGGTGATC TCTGCTACAG   601 AAAGCTGTTC ATGGTAGGCT TTCTCAGCAG AGATGACAGG GGCATATGTG GCCAGAGGGA   661 AGTGGATGCG GGGATAGGGC ACCAGGTTGG TCTGGAATTC TGTCAGGTCA ACATTCAGGG   721 CTCCATCAAA TCTCAGGGAA GCAGTGATGG AGGACACAAT TTGACCTATT AACCTATTCA   781 GGTTAGTATA GGTTGGACGC TCAATATCGA GGTTTCTACG ACAGATGTCA TAGATGGCCT   841 CATTGTCTAC CATGAAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGGTGAAGGA 901 ATGGAAGTT //
Primer: SV40pro_F_V2 Sequence file: RDB14141_A6Dpc.seq
>D03180A2_A6Dp_2_SV40pro_F_V2_F07_16.ab1     1 GTGGGCTTTT GGAGGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAACATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA   661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG   721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAACTTG GCGGCGAATG GGCTGACCGC   781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT   841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGAAA TGACCGACCA AGCGACGCCC   901 CAACCTGCCA TCACGAGATT TCGATTCCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG   961 GAATCGTTTT TCCCGGGACG CCGGCTGAAT GATCCCTCCC AGCGGCGGGG ATCTCATGGC  1021 TGCAGTTCTT CGCCAACCCT TAAGGGGAGG CTACTGCAAA CACGGAGGAG AACATACGCG 1081 GCAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl