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pGL3_hBmalPro-Luc (-3465_Full)

Reporter plasmid of human Baml 1 promoter.

Catalog number RDB14174
Resource name pGL3_hBmalPro-Luc (-3465_Full)
Alternative name 7
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -3465 to +57 (+1 is the putative transcription start site).
Comment Genomic DNA (13,274,365 – 13,277,883nt of NC_000011.10) corresponding to 1 to 157nt of NM_001178.5 and 3.4 kb up-stream was cloned. Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
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External Database
human Bmal 1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
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          RDB14174 pGL3_hBmalPro-Luc (-3465_Full) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL3_hBmalPro-Luc (-3465_Full) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14174).

          Reference section:

          Akashi, M., Takumi, T., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Circadian clock (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14174_A6F3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F (Pr0081)
          Region: insert 5'
          Sequence file: RDB14174_A6F3d.seq check
          >D03420A6_A6F3_1_pGL4-4174F_E09_15.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTCGNTAG GTNCCGAGCT CTTACGCGTG CTAGCCCGGG
             61 CTCGAGATCC AATCATTGGA TCAGAGTCAG GGAAACTACT GTAGATTATA CTACTTTATC
            121 CTCACTAACT TCATCTGTAA AACAGGAGCA AGAAATATCT ATTTCATAGA ATTGTTAAGA
            181 GTGATACCTA ATATGTATCA CATCTTGACT GTATGCCAGG CACTGTGTTA AGGGCTTTCC
            241 ATCTATCATC ACATTGAACT CTTATAACAG CCCTATGAAG TAGACACTAG ATTATCCCCC
            301 TTTTACAGAT GAGGAAACTG AGGTACAAAG AGGTTAAGCA ATTTGATAAA AGTCATCCAG
            361 CTAGCAAGTG TGGAGGGCAA GCATATCAAC TGCTAGGGTT AACGTGAGGA TTATATTAAA
            421 TAATGTATTT AAAGCATCTG GTACACAGTA GGTGCAAATA AATGCTCTCT CTTATCTTCC
            481 TTTTATCCAC ATGATTCTAC TCATGCCCAG TAAAATTTAC ATTGAGGACA ATATTTTGAC
            541 CTTAGCAGTG CCCTATGAAT TGAAAGTTCA GTATCCTGGA AAGTAGAAAA GAAAAGGGAA
            601 GTTAGGTAGT AGTGGAAGGA GGTGTTTGGG ATCTCCAACA GAGAGAACCA CAAAGAAAGG
            661 TGTAATTACA CAGGGAAAAA CAGGTGCACA TGAGGGAAGA CAGTTGCTTG NCCACTGAGG
            721 GTAGGATAAG GCAGGGTGAT AACCTTTAAA AGAGGAAAGC CAGGGTACAA GATGAGAATC
            781 TCAGANGAAG GGAGTCCACA AAGACACCTA TTTCAGCCCG CCTCCCACTG CATGGNAATC
            841 CAATTCTCAC TTGNNCATTT NNTGTGACNA GAAACTCAAT TAATCAAGGC AGAGGANGCT
            901 CTANCTGNTA NAAAANTGTN CNTATNGATT CANNCTNNAN TTTCTANCNN CANGNNNNNC
            961 NNCNNCCCNN TNNNNNCCTT NAGTATNNGT TNNNNTNNNN NNCNNNTNNA NANNNNNNNN
          1021 NN
          //
          Primer: Luc_R (Pr0146)
          Region: luc,insert 3'
          Sequence file: RDB14174_A6F3e.seq check
          >D03420A6_A6F3_1_Luc_R_H01_22.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
             61 TCCNTCTTCC AGCGGANAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
            121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
            181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCTGACCT ACTTTCTGCC
            241 TTCCCTAAAT GTCGCACCAC TAACCTACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
            301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
            361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
            421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
            481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
            541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
            601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCAGG TCTCCCACTA
            661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGNGGT CGCCCGGGAT GTCCCTCTTC
            721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
            781 TCCCCTCCGG GCATTCCGAC GNNNNGCAGC ANTTCCNGAC TCCCTCTCTC CCTCNGGCTN
            841 NNTCTANGAA ANCNNNTTTN ANGNNCGTTN NNNGCCAGAN CCCNTNNCNA AAANNCCCNN
          901 GNNTNNNCAA ANNNNNNANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743. [link to RRC of NBRP]

          2024.03.26

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