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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_CD19 (#RDB14511)

Retroviral expression vector of mouse CD19.

Clone info. Retroviral expression vector of mouse CD19, cloned into the pMXs-IRES-GFP. Refer Fig.2 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Cd19 cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14511 pMXs-IRES-GFP_CD19 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_CD19 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14511).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14511_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14511_A6Lea.seq check
>D04073B2_A6Le_3_pBABE5'_A11_02_ABI24.ab1
    1 GTCCTATATG GCATATGAGA TCTTATATGG GGCACCCCCG CCCCTTGTAA ACTTCCCTGA
   61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
  121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
  181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
  241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
  301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
  361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCCAGTGTG GTGGTACGGG AATTCCTGCA
  421 GGCCTCGAGC ACCATGCCAT CTCCTCTCCC TGTCTCCTTC CTCCTCTTTC TTACCTTAGT
  481 AGGAGGCAGG CCCCAGAAGT CCTTACTGGT GGAGGTAGAA GAGGGAGGCA ATGTTGTGCT
  541 GCCATGCCTC CCGGACTCCT CACCTGTCTC TTCTGAGAAG CTGGCTTGGT ATCGAGGTAA
  601 CCAGTCAACA CCCTTCCTGG AGCTGAGCCC CGGGTCCCCT GGCCTGGGAT TGCACGTGGG
  661 GTCCCTGGGC ATCTTGCTAG TGATTGTCAA TGTCTCAGAC CATATGGGGG GCTTCTACCT
  721 GTGCCAGAAG AGGCCCCCTT TCAAGGACAT CTGGCAGCCT GCCTGGACAG TGAACGTGGA
  781 GGATAGTGGG GGAGATGTTC CGGTGGAATG CTTCAGACGT CAGGGACCTG GACTGTGACC
  841 TAAGGAACAG GTCCTCTGGG AGCCACAGGT CCACTTCTGG TTCCCAGCTG TATGTGTGGG
  901 CTAAAGACCA TCCTAAGGTC TGGGGAACAA AGCCTGTATG TGCCCCTCGG GGGAGCAGTT
  961 TGAATCAGAG TCTAATCAAC CCAAGATCTC ACTGTGGCAC CCGGGCTCCA CACTTTGGCT
 1021 GTCCTGTGGG GTACCCCCTG TCCCAGTGGT CAAAAGGCTC CATCTCCTGA CCCATGTGCC
 1081 ATCTAGAGAC TAATGTTTTC ACTTACTGAG CTTAGCTGGG GAAGAGCACC GGTCAGAAGA
1141 GTATGGTTGG GGCTTTTCG
//
Primer: IRES-R
Sequence file: RDB14511_A6Leb.seq check
>D04073B2_A6Le_3_IRES-R_B11_05_ABI24.ab1
    1 GCCAACAGCG GCTTCGGCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG
   61 AATTTACGTA GCGGCCGCTC ACGTGGTTCC CCAAGTCCCC ATGTGGCCCT CTCCTTCCCA
  121 TGCTGGTTCT AGGTCGTCAG ACTTATCCAT GTTTTCATAA GAGTCTGCAT CTTCTTCATG
  181 ACTGGGACCG GACTGAATTG AGTGGAGCTG AGGAGCTGCA TAGAGGATCC CTCTCATATC
  241 TTCATAGGAC TGGGACCCAA GCGAGGATGC TTCCCGGCTG GGGTCCCACG CAGACCCATG
  301 GGGGCTCAGG AAGTCCATCA TCCTGCCAAC TGGTTGGGCC AGCTCCTCAT CTGCATTTTC
  361 ATAAGACTCA GCATTGGAGA AGGAGTCTTC TTCCTCTGGA CCCATGGGCT CATCCTCGGG
  421 GTTCTCATAG CCACTCCCAT CCTGGGAAAC CTGGTCCTGC CCAAGGTTGG AGTCGTTCTC
  481 ATAGAATTCA GAGCCCTCCT CGCTGTCTGG CTCTTCATAG GCCTCCCCTT CTTCTTCCAG
  541 TCCTGTTTCA TGGCTCTGAG CTCCAGTATC CTGGACTTGA ATGCGTGGAT TTCCATAGGA
  601 CCCAGGGACA CTCCCTAGGC CAGCAGCCCA ACGCTGAGCA TGGGCCTGGC CAGAGGTAGA
  661 TGTAGGAAGG GAGAGCACAT TCCCGTACTG GTTCTGGGTC CCGTTTCCCG AGGGAGGCGT
  721 CACTTTGAAG AATCTCCTGG CGGGGTCAGT CATTCGCTTC CTTTTCCTTC TCAGGATAAA
  781 GGCTCTTTGA CAATAGAGAA AAGCCACCAG AGAAACCATA CAGAAGATGA CATATACTAA
  841 AGTCACCACT GGGACTATCC ATCCACCAGT TCTCAACAGC CAGAGCCACA CTGCTGACCT
  901 TGCAATGACC TTCACGTGCC TCTCGATGGT CAGGTTTCCT CGGAGACATA ATAGGTGCCT
  961 TCATCTAAAG CTGTGGCTTG GGCAGAGCAG AAGAGACCCC CAAACCCACA TCTCTCTGAC
 1021 GGGTGCTCTC CCCAAGCTAG CTCAGTTAGT GAACATAGTC TCTAGGATGC ACATGGTCAG
1081 CAGATGGAGC TACACTGGAC AGGGTACCCA CAGAACAGCC AAGTGGTGGG A
//
Primer: IRES-F
Sequence file: RDB14511_A6Lec.seq check
>D04073B2_A6Le_3_IRES-F_C11_08_ABI24.ab1
    1 TCAAGCTTTC ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGAATGA
  901 AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA
  961 AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGACAGATG GGAACAGCTG
 1021 ATATGGGCCA AACAGGATAT CTGTGGTAGC AGTCCTGCCC CGGGCTTCAA GTAGAACAGA
 1081 TGACAGCTGG ATATGGCCAA CAGATTTCTT GTGTTAGCAG TTTCCTGGAC CCCGGGCTCA
//
Primer: Amp_R
Sequence file: RDB14511_A6Led.seq check
>D04073B2_A6Le_3_Amp_R_H04_22_ABI24.ab1
    1 CTCCTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
  961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA ATCCCGATCG TTTTGGACTC TTTGGTGCAC
 1021 CCCCTTAGAG GAGGGATATG TGGTCTGGTA GGAGACGAGA ACTAAAACAG TCCGCTCGTC
 1081 TGAGTTTTGC CTTTCCGTTG GAACGGAGCC GCGCGGCGTC TTGCTGCTGC AGCATCGCTC
1141 GTGATGCTCT GGTCTGGACT GGTGATTTCC GGTATTC
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14511_A6Lee.seq check
>D04073B2_A6Le_3_SV40pA3'element Reverse_H05_23_ABI24.ab1
    1 GATTTCAAGG GGGGGCGGAC CTATGGAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
   61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
  121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
  181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
  241 CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT AAATACTTAA GAAAAAAAAC
  301 CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  361 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  421 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
  481 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  541 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC AGGTCCTTGG GGCACCCTGG
  601 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC CGCATCTGGG GACCATCTGT
  661 TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC
  721 AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT
  781 GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT GATCTGAACT TCTCTATTCT
  841 CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT
  901 ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA AATAAAATCT TTTATTTTAT
  961 CGTCGACCAC TGTGCTGGCG GCCGGCCGCT TTACTTGTAC AGCTCGTCCA TGCCGAGAGT
 1021 GATCCCGGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTGGGGTC
 1081 TTTGCCTCAG CGACTGGGTG CTCAGTAGTG GATGTCCGGC CAGCCAGCAC GGGGCTCGTC
1141 CGCTCGATGG GGGGTGTTCT CTTGCCTTGG ATAAGTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]