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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_CD19-2YF (#RDB14512)

Retroviral expression vector of mouse CD19-2YF mutation in the two YxxM motifs (Y482 and Y513).

Clone info. Retroviral expression vector of mouse CD19-2YF mutant, in which two YxxM motifs (Y482 and Y513; PI3K-binding motif) were replaced with FxxM motifs, cloned into the pMXs-IRES-GFP. Refer Fig.2 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Cd19 cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14512 pMXs-IRES-GFP_CD19-2YF DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_CD19-2YF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14512).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14512_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14512_A6Lea.seq check
>D04073B3_A6Le_1_pBABE5'_F01_16_ABI24.ab1
    1 GTCTTAATAT GGCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
   61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
  121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
  181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
  241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
  301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
  361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCCTGC
  421 AGGCCTCGAG CACCATGCCA TCTCCTCTCC CTGTCTCCTT CCTCCTCTTT CTTACCTTAG
  481 TAGGAGGCAG GCCCCAGAAG TCCTTACTGG TGGAGGTAGA AGAGGGAGGC AATGTTGTGC
  541 TGCCATGCCT CCCGGACTCC TCACCTGTCT CTTCTGAGAA GCTGGCTTGG TATCGAGGTA
  601 ACCAGTCAAC ACCCTTCCTG GAGCTGAGCC CCGGGTCCCC TGGCCTGGGA TTGCACGTGG
  661 GGTCCCTGGG CATCTTGCTA GTGATTGTCA ATGTCTCAGA CCATATGGGG GGCTTCTACC
  721 TGTGCCAGAA GAGGCCCCCT TTCAAGGACA TCTGGCAGCC TGCCTGGACA GTGAACGTGG
  781 AGGATAGTGG GGGAGATGTT CCGGTGGAAT GCTTCAGACG TCAGGGACCT GGACTGTGAC
  841 CTAAGGAACA GGTCCTCTGG GAGCCACAGG TCCACTTCTG GTTCCCAGCT GTATGTGTGG
  901 GCTAAAGACC ATCCTAAGGT CTGGGGAACA AAGCCTGTAT GTGCCCCTCG GGGGAGCAGT
  961 TTGAATCAGA GTCTAATCAA CCAAGATCTC ACTGTGGCAC CCGGGCTCCA CACTTTGGCT
 1021 GTCCTGTGGG GTACCCCCTG TCCCAGTGGG CCAAAGGCTC ATCTCCTGGA CCCATGTGCC
 1081 ATCTAGAGAC CTAATGTTTT CACTACTTGA GCTAGTCATG GGGGAGAGCA CCTCGTCAGA
1141 AGAAAGAATG GTTGGGCTTT TG
//
Primer: IRES-R
Sequence file: RDB14512_A6Leb.seq check
>D04073B3_A6Le_1_IRES-R_D03_12_ABI24.ab1
    1 GCATCAGGCG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCTCA CGTGGTTCCC CAAGTCCCCA TGTGGCCCTC TCCTTCCCAT
  121 GCTGGTTCTA GGTCGTCAGA CTTATCCATG TTTTCAAAAG AGTCTGCATC TTCTTCATGA
  181 CTGGGACCGG ACTGAATTGA GTGGAGCTGA GGAGCTGCAT AGAGGATCCC TCTCATATCT
  241 TCAAAGGACT GGGACCCAAG CGAGGATGCT TCCCGGCTGG GGTCCCACGC AGACCCATGG
  301 GGGCTCAGGA AGTCCATCAT CCTGCCAACT GGTTGGGCCA GCTCCTCATC TGCATTTTCA
  361 TAAGACTCAG CATTGGAGAA GGAGTCTTCT TCCTCTGGAC CCATGGGCTC ATCCTCGGGG
  421 TTCTCATAGC CACTCCCATC CTGGGAAACC TGGTCCTGCC CAAGGTTGGA GTCGTTCTCA
  481 TAGAATTCAG AGCCCTCCTC GCTGTCTGGC TCTTCATAGG CCTCCCCTTC TTCTTCCAGT
  541 CCTGTTTCAT GGCTCTGAGC TCCAGTATCC TGGACTTGAA TGCGTGGATT TCCATAGGAC
  601 CCAGGGACAC TCCCTAGGCC AGCAGCCCAA CGCTGAGCAT GGGCCTGGCC AGAGGTAGAT
  661 GTAGGAAGGG AGAGCACATT CCCGTACTGG TTCTGGGTCC CGTTTCCCGA GGGAGGCGTC
  721 ACTTTGAAGA ATCTCCTGGC GGGGTCAGTC ATTCGCTTCC TTTTCCTTCT CAGGATAAAG
  781 GCTCTTTGAC AATAGAGAAA AGCCACCAGA GAAACCATAC AGAAGATGAC ATATACTAAA
  841 GTCACCACTG GGACTATCCA TCCACCAGTT CTCAACAGCC AGAGCCACAC TGCTGACCTT
  901 GCAATGACCT TCACGTGCCT CTCGATGGTC AGGTTTCCTC GGAGACAATA ATAGGTGCCT
  961 TCATCTAAAG CTGTGGCTTG GGGGCAGAAG CAGAAGAGAC CCCCAAAACC CACATCTCTC
 1021 TGACCGGGGT GCTCTCCCCC CAAGGCTTTA GGCTCAGTAG TGAAACATTA GGTCTCCTAG
 1081 GGATGCACAT GGGTCCAGGG AGATGGGAGC TTGACAACTG CAACAGGGGG TACCCCACAG
1141 GACCAGCCAA AAGTGTTTGA GTG
//
Primer: IRES-F
Sequence file: RDB14512_A6Lec.seq check
>D04073B3_A6Le_1_IRES-F_H08_23_ABI24.ab1
    1 GGAAGTCTTT ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA AGGGGGGAAT
  901 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCCATTT TTGCAAGGCA
  961 TGGAAAAATT ACATAAACTG AGAATAGAGA AAGTTCAGAT CAAGGTCAGA CAGATGGGAA
 1021 CAGCTGATTA TGGGCCCAAA CAGGATTATC TGTGGTAGGC AGGTTTCCTG CCCCGCCTCA
1081 GTCAGATCAG ATGGAAACAG CCTGAAATAT TTGGGGCCC
//
Primer: Amp_R
Sequence file: RDB14512_A6Led.seq check
>D04073B3_A6Le_1_Amp_R_F09_18_ABI24.ab1
    1 CTCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
  961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA AAATCCCGAT CGTTTTGGGA CTCTTTGGTG
 1021 CAACCCCCTT TAGAGAAGGA TATGTGATCC TGTTAGGAGA CGAGCACCTT AAACAGTCCG
1081 CTCCGTCTGG ATTTTTTGCT TTTCCGG
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14512_A6Lee.seq check
>D04073B3_A6Le_1_SV40pA3element Reverse_G09_21_ABI24.ab1
    1 GAGTAGGGGG CGGACCTATG GAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
   61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
  121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
  181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
  241 CCGATTCATT AATGCAACTA TGGCCATTTA ATGTAAATAC TTAAGAAAAA AAACCAAATT
  301 AATTTTGATA CATGCTGCAT GTGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
  361 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
  421 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCCCCGAG TGAGGGGTTG
  481 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
  541 ATTGGTTAGT TCAAATAAGG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT
  601 CTGATGGTTC TCTAGAAACT GCTGAGGGCT GGACCGCATC TGGGGACCAT CTGTTCTTGG
  661 CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT
  721 TCCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG
  781 CCCATATTCA GCTGTTCCAT CTGTTCCTGA CCTTGATCTG AACTTCTCTA TTCTCAGTTA
  841 TGTATTTTTT CCATGCCTTG CAAAATGGCG TTACTTAAGC TAGCTTGCCA AACCTACAGG
  901 TGGGGGTCTT TCATTCCCCC CCTTTTTTCT GGAGACTAAA TAAAATCTTT TTATTTTTAT
  961 CGTCGACCAC TGTGCTGGGC GGGCCCGGGC CGCTTTTACT TTGTACAGCT CGTCCCATGC
 1021 CCGAGAGGTG ATCCTGGCGC GGGTCACGAA CTCAAGCCAA GACCCATGTG ATTCGCGCAT
1081 CTCGATGGGG GATCTTTGCC TCCAGGGGCC CGGTAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]