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pGL4_ifng-1500-luc (#RDB14517)

Reporter construct of mouse interferon gamma (Ifng) promoter (-1500 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-1500 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-1500-luc, the PCR product (1.6 kb) was amplified with forward primer (5' CTA CTC GAG TCT GAT TTG AAG CTG GAG GC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,439,542 to 118,441,154 nt of NC_000076.6 is cloned. Known difference (at least): A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14517 pGL4_ifng-1500-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-1500-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14517).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14517_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB14517_A6Lfa.seq check
>D03972A3_A6Lf_1_pGL4-4174F_G07_07_ABI08.ab1
    1 GAGGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGTC
   61 TGATTTGAAG CTGGAGGCTT GGGGGAGAAC CAAAACAGAG GATGGTTTGC ATCTGGGTCA
  121 AGATAACTGG GTACCACTCT GAGATGCTCA GATGAGGGAA AGGGGGCACA TGGCCAAAGG
  181 AACTGCACGA ACAAGCTGCA TAGCGTTGTT AAACATCTGA CATTGCCAAA TAACCATCAA
  241 GTTTTAATTG AGCCACTAGG AATGCCGGGA AGAATTCTCT CAACTTTCCA TGGAGCATTT
  301 GGTTGAGCCT TTGGCTGAAG TCCGGAAGAA AATGCTAGTG TTAAGCTCAC TGTATAAATG
  361 TGGAAACTGA GTCACAGATC ATAAAGATGC TGACTCAAGA CCCCGAGGCT AGAATATGAA
  421 TGGTTCAAGT CTGCACCCAT AGCCACTGTG TAATGTTAAT GAATACAGGA AAGGCAGGGA
  481 TGAATACACG TGGGCTGGCA CTGAAGAGCA GAGGGCCTCC TGCCCCATCC TCAAGGATCG
  541 AATTTCATAG ATAAGTTACC AAAGACAGAG AAGGAAACTT TTAGTGTTTG AACTTGATGG
  601 GGGAAACAAT TTTCAGTTAT TAATCCTTAT TTGGGACAAG TGTGTGTCTA GTGCTGGACT
  661 AGTCATTGGG ATAGCCTTAG CCACATGTGG GTATGAGAGT TAGAAACATG GCTAATCCTG
  721 AAGTGATTTC TGCTGTGTAT ATAGGATAGC CACTAGGCTT TAAATATTTA GTGGCAGAGG
  781 AATTGAAACT CTTATTCATC ATAAATCTTG ATACACATTG ATACCTTGGG TGTGTTGAGT
  841 GAAATACAAT ATCTAATGAA GTATTCTTGC ATTTTTTACT TTTATTTCAT TTTTGCATTT
  901 TTTTCCCTTT TTATTAATGG AGGAACTAGA TGATTTTTAT TTTTACCCTA TGTGGTCTGC
  961 CCTTTTTCTT TCTTCTGGGC ACGTTTGACC CTGAGTGATT TGTAGTAGGT ATTTTTACCT
 1021 AATCACTCCA TTTGAACGGA CTTCGTCACA CATGGCCTTT TTTATCATAC GTTCCTGGCT
1081 TATCTGGTCA TTAGCAGGAC CAGCTTTGCC GC
//
Primer: Luc_R ()
Region: insert 3'
Sequence file: RDB14517_A6Lfb.seq check
>D03972A3_A6Lf_1_Luc_R_H07_08_ABI08.ab1
    1 GTTGTAAGGT GCCGGGGGCA ACAGGGCGTA GCGCTTCATG GCTTTGTGCA GCTGCTCGCC
   61 GGCGGTCCCG TCTTCGAGTG GGTAGAATGG CGCTGGGCCC TTCTTAATGT TTTTGGCATC
  121 TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT TGGCCGCCGA GGCCAGATCT
  181 TGATATCTGT CTCAGAGCTA GGCCGCAGGA GGAGAAGCCC AGAACTTCTG TCTCAAGTCA
  241 GAGGGTCCAA AGGATCAGCT GATGTGTCTT CTCTAGGTCA GCCGATGGCA GCTATAGCCT
  301 GCACCTCTCT GGCTTCCAGT TTTATACCTG ATCGAAGGCT CCTCGGGATT ACGTATTTTC
  361 ACAAGTTTTT TTTTGGTTTT TTTTTTTTTT TTAAGATGGT GACAGATAGG TGGGGGGAGC
  421 TAAGTTACTT TGCATTACAG CTATGGTTTT GTGGCATGTT AGAGTTTCCT TTCGACTCCT
  481 TGGGCTCTCT GACGATGAGA CAGCCCCGCT GTGCCCACCT GTGCCATTCT TGTGGGATTC
  541 TCTGAAAGCA CTTTTACTTC ACACCATTTG GGGTGGGGGC TGGAAATTGC TTTTACGTCT
  601 CCCCCTTTTT GCCCTTGTAA TGTGAATTTC TCATCCACAG AGCACAGCAC AGGGAGCCTT
  661 TGTTTGTGGT TGGGGACGGC TGAATAAAAG GAAACTCTTG GGCTTCTCAA ACCATGAGGA
  721 AACTCTTGGG CTTCTCAAAC CATGCAATGG GGGAAGCCTT TTCAAACTTT TCTTGCAAAT
  781 GACCAGAAAA GCAAGGAAAG GGTATGGTTA AAAAGCCAAT GTGGTGAGGA AGCCCTTCAT
  841 GGGAGGTGAT TAGTAAAAAT ACCTACTACA AATCACTCAG GGTCAACGTG CCCAGAAAGA
  901 AGAAAAGGCA GACCACATAG GGTAAATAAA TCATCTAGTT TCCTCCCATT AATAAAAGGG
  961 AAAAAATGCA AATGGAATTA AAAGTAAAAA ATGCCAAGAA TACCTTCATT AAGATAATTG
 1021 TATTTTCCAC TCAACACACC CCCAGTAATC CAATGTGTTA ATCAAGATTT ATGAATGGAA
1081 TAAGAGGTTA CATTCGCTGG CCCACTTAAA TATTTAAGGC CGTAAGTGGC CTATATC
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14517_A6Lfc.seq check
>D03972A3_A6Lf_1_SV40pro_R2_E09_15_ABI24.ab1
    1 ATCATGCATT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
   61 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGAAGGTA GCTAACCAAG TTCCTCTTTC
  121 AGAGGTTATT TCAGGCCATG GTGCTGCGCA GATCAGCGGA AGAGCGCCCA ATACGCAAAC
  181 GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
  241 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
  301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGCT
  421 CGAAGCGGCC GGCCGCCCCG ACTCTAGACT GCAGTTAGAC GTTGATCCTG GCGCTGGCGC
  481 AAGCAGCAGG GTGTCTATCC ATGCCGCTCT CCTGGGCGCA GCTCATGGGC AGGGTGCCGG
  541 CGGCCTGCTC CTCCACCTCG GGAGGGAAGC CGTGGCTGTT AAGGTGGATC ACAAAGTGGC
  601 TTAAGCTACT GAACCAGTTC TTGCAAGCAG AATTCACGGC GATCTTGCCG CCCTTCTTGG
  661 CCTTAATGAG AATCTCGCGG ATCTTGCGGG CGTCCAACTT GCCGGTCAGT CCTTTAGGCA
  721 CCTCGTCCAC GAACACAACA CCACCGCGCA GCTTCTTGGC GGTTGTAACC TGGCTGGCCA
  781 CATAGTCCAC GATCTCCTTC TCGGTCATGG TTTTACCGTG TTCCAGCACG ACGACTGCGG
  841 CGGGCAGCTC GCCGGCATCG TCGTCGGGCA GGCCGGCGAC CCCGGCGTCG AAGATGTTGG
  901 GGTGTTGCAG CAGATGCTCT CCAGTTCGGC TGGGGCTACC TGGTAGCCCT TGTATTTGAT
  961 CAGCTCTTCA GCCGGTCCAC GATGAAGAAA GTGCTCGTCC TCGTCCCAGT AGCGATGTCG
 1021 CCCGCTGTGC AGCCAGCCCG TCCCTTGTCG ATGAGAGCGT TTGTAGCCTC AGGTGTACGT
 1081 AGCCGCTCAT GAATCATGGG CACGGAACGC CACAGCTCGC GCGCTGGATT CAACACCCCA
1141 GGGTATC
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14517_A6Lfd.seq check
>D03972A3_A6Lf_1_SV40pro_F_A08_01_ABI08.ab1
    1 CTTGGTCCTG TACAATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGACCGAGT ACAAGCCTAC CGTGCGCCTG
  121 GCCACTCGCG ATGATGTGCC CCGCGCCGTC CGCACTCTGG CCGCCGCTTT CGCCGACTAC
  181 CCCGCTACCC GGCACACCGT GGACCCCGAC CGGCACATCG AGCGTGTGAC AGAGTTGCAG
  241 GAGCTGTTCC TGACCCGCGT CGGGCTGGAC ATCGGCAAGG TGTGGGTAGC CGACGACGGC
  301 GCGGCCGTGG CCGTGTGGAC TACCCCCGAG AGCGTTGAGG CCGGCGCCGT GTTCGCCGAG
  361 ATCGGCCCCC GAATGGCCGA GCTGAGCGGC AGCCGCCTGG CCGCCCAGCA GCAAATGGAG
  421 GGCCTGCTTG CCCCCCATCG TCCCAAGGAG CCTGCCTGGT TTCTGGCCAC TGTAGGAGTG
  481 AGCCCCGACC ACCAGGGCAA GGGCTTGGGC AGCGCCGTCG TGTTGCCCGG CGTAGAGGCC
  541 GCCGAACGCG CCGGTGTGCC CGCCTTTCTC GAAACAAGCG CACCAAGAAA CCTTCCATTC
  601 TACGAGCGCC TGGGCTTCAC CGTGACCGCC GATGTCGAGG TGCCCGAGGG ACCTAGGACC
  661 TGGTGTATGA CACGAAAACC TGGCGCCTAA TGATCTAGAA CCGGTCATGG CCGCAATAAA
  721 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT
  781 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
  841 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
  901 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
  961 AGCGGGTATC AGCTCACTCA AAGGCGGTAT ACGGTTATCC ACAGAATTCA GGGATACGCA
 1021 AGAAGGACAT GTGAAGCCAA AGTCAGCCAA AAGCCAGAAC CGTAAAAGCG CGATGCTTGC
1081 CGATTTTTCA TTAGGATT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]