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pGL4_ifng-36-luc (#RDB14520)

Reporter construct of mouse interferon gamma (Ifng) promoter (-36 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-36 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-36-luc, the PCR product (0.1 kb) was amplified with forward primer (5' CTA CTC GAG TTC GAT CAG GTA TAA AAC TGG 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14520 pGL4_ifng-36-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-36-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14520).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14520_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert
Sequence file: RDB14520_A6Lfa.seq check
>D03972A6_A6Lf_1_pGL4-4174F_G08_07_ABI08.ab1
    1 ATGAGGGTCC CAGGACATTT CTCTGGCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
   61 GTTCGATCAG GTATAAAACT GGAAGCCAGA GAGGTGCAGG CTATAGCTGC CATCGGCTGA
  121 CCTAGAGAAG ACACATCAGC TGATCCTTTG GACCCTCTGA CTTGAGACAG AAGTTCTGGG
  181 CTTCTCCTCC TGCGGCCTAG CTCTGAGACA GATATCTAGA TCAAGATCTG GCCTCGGCGG
  241 CCAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA
  301 GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA
  361 AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA
  421 GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA
  481 GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT
  541 CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT
  601 CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT
  661 GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA
  721 GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT
  781 GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG
  841 GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGGCG
  901 TAGCCCCTAC CGCACCGCAC CGCTTGTGTC CGATTTCAGT CATGCCCGCG ACCCCCATCT
  961 TCGGGCAACC AGATCATCCC CGACACCGCT ATCCCTCAGC GTGGTGCCAT TTCACCCACG
 1021 GCTTTCGCAT GTTTCCACCA CGCTGGGCTA CTTGATCCTG CCGGCTTTCG GGGTCGTGCT
1081 TCATGTAACG CTTCGGAGGC AGAGCTAATT CCGTGCCTAG CTGGCAGACC TAATAGAG
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14520_A6Lfb.seq check
>D03972A6_A6Lf_1_SV40pro_R2_H09_24_ABI24.ab1
    1 AGCAAGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA
   61 CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGAAGGTAG CTAACCAAGT TCCTCTTTCA
  121 GAGGTTATTT CAGGCCATGG TGCTGCGCAG ATCAGCGGAA GAGCGCCCAA TACGCAAACG
  181 GATCCTTATC GATTTTACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA
  241 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC
  301 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
  361 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGCTC
  421 GAAGCGGCCG GCCGCCCCGA CTCTAGACTG CAGTTAGACG TTGATCCTGG CGCTGGCGCA
  481 AGCAGCAGGG TGTCTATCCA TGCCGCTCTC CTGGGCGCAG CTCATGGGCA GGGTGCCGGC
  541 GGCCTGCTCC TCCACCTCGG GAGGGAAGCC GTGGCTGTTA AGGTGGATCA CAAAGTGGCT
  601 TAAGCTACTG AACCAGTTCT TGCAAGCAGA ATTCACGGCG ATCTTGCCGC CCTTCTTGGC
  661 CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG CCGGTCAGTC CTTTAGGCAC
  721 CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG GTTGTAACCT GGCTGGCCAC
  781 ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT TCCAGCACGA CGACTGCGGC
  841 GGGCAGCTCG CCGGCATCGT CGTCGGGCAG GCCGGCGACC CCGGCGTCGA AGATGTTGGG
  901 GTGTTGCAGC AGATGCTCTC CAGTTCGGCT GGGGCTACCT GGTAGCCCTT GTATTTGATC
  961 AGGCTCTTCA GCCCGGTCCA CGATGAAGAA GTGCTCGTCC TCGTCCCAGT AGGCGATGTC
 1021 GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT GAGAGCGTTT GTAGCCCTCC GGGGATGTAC
 1081 GTAGCCGCTC ATGAATCATG GGGCACGAAC GCCACAGCTT CGGCCGCGCT TGGAATCACA
1141 CACCCCAGTG C
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14520_A6Lfc.seq check
>D03972A6_A6Lf_1_SV40pro_F_C09_03_ABI08.ab1
    1 CCCTCGGTCC CTGGGCATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGACCG AGTACAAGCC TACCGTGCGC
  121 CTGGCCACTC GCGATGATGT GCCCCGCGCC GTCCGCACTC TGGCCGCCGC TTTCGCCGAC
  181 TACCCCGCTA CCCGGCACAC CGTGGACCCC GACCGGCACA TCGAGCGTGT GACAGAGTTG
  241 CAGGAGCTGT TCCTGACCCG CGTCGGGCTG GACATCGGCA AGGTGTGGGT AGCCGACGAC
  301 GGCGCGGCCG TGGCCGTGTG GACTACCCCC GAGAGCGTTG AGGCCGGCGC CGTGTTCGCC
  361 GAGATCGGCC CCCGAATGGC CGAGCTGAGC GGCAGCCGCC TGGCCGCCCA GCAGCAAATG
  421 GAGGGCCTGC TTGCCCCCCA TCGTCCCAAG GAGCCTGCCT GGTTTCTGGC CACTGTAGGA
  481 GTGAGCCCCG ACCACCAGGG CAAGGGCTTG GGCAGCGCCG TCGTGTTGCC CGGCGTAGAG
  541 GCCGCCGAAC GCGCCGGTGT GCCCGCCTTT CTCGAAACAA GCGCACCAAG AAACCTTCCA
  601 TTCTACGAGC GCCTGGGCTT CACCGTGACC GCCGATGTCG AGGTGCCCGA GGGACCTAGG
  661 ACCTGGTGTA TGACACGAAA ACCTGGCGCC TAATGATCTA GAACCGGTCA TGGCCGCAAT
  721 AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC
  781 TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC
  841 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG
  901 CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
  961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
 1021 CGCAGGAAAG ACATGTGAGC AAAAGGCCAG CAAAGGCCAG GACCGTAAAA GGCCGCGTGC
 1081 TGCGTTTTTC ATAGCTCGCC CCCTGACGAG CATCCAAAAT CGAACGCCTC AGTCGAGGTG
1141 GCCGAACCGA CATGACTATA AAGAATACCA AGGGCGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]