Expression vector of human ARL6ip1 plus Gluc inserted after Arg at 189aa.
Clone info. | Expression vector of human ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) plus Gluc. Gluc was inserted after Arg at 189aa. The structure of the plasmid construct, see Fig. 1B and C of Kuroda, M. et al. FEBS Lett., 587 (22): 3656-3660, 2013. |
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Comment | Expression was confirmed by the depositor with redox-sensitive luciferase assay. |
Vector backbone | pCI-neo (plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells). |
Gene/insert name | human ARL6IP1 cDNA Gaussia princeps Luciferase cDNA |
Depositor|Developer | Simizu, Siro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Masahiro, K. et al. FEBS Lett. 587, 3656-3660, 2013) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masahiro, K. et al. FEBS Lett. 587, 3656-3660, 2013)。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB14561 | pCI-neo/ARL6ip1(1-189)-Gluc-ARL6ip1(190-203) [R189] | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCI-neo/ARL6ip1(1-189)-Gluc-ARL6ip1(190-203) [R189] was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14561). |
Reference section:
Kuroda, M., Funasaki, S., Saitoh, T., Sasazawa, Y., Nishiyama, S., Umezawa, K., Simizu, S., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. [link to RRC of NBRP] |
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