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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-Rab18 (#RDB14699)

Expression vector of human Rab18, wild type, fused with mCherry.

Clone info. Expression vector of human Rab18, wild type, fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14699 pDEST/mCherry-Rab18 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-Rab18 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14699).

Reference section:

Makino, A., Hullin-Matsuda, F., Murate, M., Abe, M., Tomishige, N., Fukuda, M., Yamashita, S., Fujimoto, T., Vidal, H., Lagarde, M., Delton, I., Kobayashi, T., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14699_A7Djp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F ()
Sequence file: RDB14699_A7Dja.seq check
>D04483A3_A7Dj_1_mCherry-C_F_A05_02_ABI24.ab1
    1 TAACAAGCAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
   61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
  121 ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT TGTACAAAAA AGCAGGCTCC
  181 GCGGCCGCCT TGTTTAACTT TAAGAAGGAG CCCTTCACCA TGGACGAGGA CGTGCTAACC
  241 ACCCTGAAGA TCCTCATCAT CGGCGAGAGT GGGGTGGGCA AGTCCAGCCT GCTCTTGAGG
  301 TTCACAGATG ATACGTTTGA TCCAGAACTT GCAGCAACAA TAGGTGTTGA CTTTAAGGTG
  361 AAAACAATTT CAGTGGATGG AAATAAGGCT AAACTTGCAA TATGGGATAC TGCTGGTCAA
  421 GAGAGGTTTA GAACATTAAC TCCCAGCTAT TATAGAGGTG CACAGGGTGT TATATTAGTT
  481 TATGATGTCA CAAGAAGAGA TACATTTGTT AAACTGGATA ATTGGTTAAA TGAATTGGAA
  541 ACATACTGTA CAAGAAATGA CATAGTAAAC ATGCTAGTTG GAAATAAAAT CGATAAGGAA
  601 AATCGTGAAG TCGATAGAAA TGAAGGCCTG AAATTTGCAC GAAAGCATTC CATGTTATTT
  661 ATAGAGGCAA GTGCAAAAAC CTGTGATGGT GTACAATGTG CCTTTGAAGA ACTTGTTGAA
  721 AAGATCATTC AGACCCCTGG ACTGTGGGAA AGTGAGAACC AGAATAAAGG AGTCAAACTG
  781 TCACACAGGG AAGAAGGCCA AGGAGGAGGA GCCTGTGGTG GTTATTGCTC TGTGTTATAA
  841 AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TTGATCTAGA GGGCCCGCGG
  901 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
  961 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
 1021 CAGCATCTGT GTTGCCCCTC CCCCGTGCCT TCATGACCTG AGTGCCACTC CCACTGTCGT
 1081 TCTTATTAAT GAAGAAATGC ATCGCATTGT TGATAGTTCA TTCTATCTGG GGGGTTGGGA
1141 TTGAGCCAGG AA
//
Primer: BGH_rev2 ()
Sequence file: RDB14699_A7Djb.seq check
>D04483A3_A7Dj_1_BGH_rev2_B05_05_ABI24.ab1
    1 GTATTCCATA AGCAAGTAAC ACGTCACACA GGGCAGCAGC AGATGGCGTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACTCAATGGT GATGGTGATG ATGACCGGTA
  121 CGCGTAGAAT CGAGACCGAG GAGAGGGTTA GGGATAGGCT TACCTTCGAA CCGCGGGCCC
  181 TCTAGATCAA CCACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT TTATAACACA
  241 GAGCAATAAC CACCACAGGC TCCTCCTCCT TGGCCTTCTT CCCTGTGTGA CAGTTTGACT
  301 CCTTTATTCT GGTTCTCACT TTCCCACAGT CCAGGGGTCT GAATGATCTT TTCAACAAGT
  361 TCTTCAAAGG CACATTGTAC ACCATCACAG GTTTTTGCAC TTGCCTCTAT AAATAACATG
  421 GAATGCTTTC GTGCAAATTT CAGGCCTTCA TTTCTATCGA CTTCACGATT TTCCTTATCG
  481 ATTTTATTTC CAACTAGCAT GTTTACTATG TCATTTCTTG TACAGTATGT TTCCAATTCA
  541 TTTAACCAAT TATCCAGTTT AACAAATGTA TCTCTTCTTG TGACATCATA AACTAATATA
  601 ACACCCTGTG CACCTCTATA ATAGCTGGGA GTTAATGTTC TAAACCTCTC TTGACCAGCA
  661 GTATCCCATA TTGCAAGTTT AGCCTTATTT CCATCCACTG AAATTGTTTT CACCTTAAAG
  721 TCAACACCTA TTGTTGCTGC AAGTTCTGGA TCAAACGTAT CATCTGTGAA CCTCAAGAGC
  781 AGGCTGGACT TGCCCACCCC ACTCTCGCCG ATGATGAGGA TCTTCAGGGT GGTTAGCACG
  841 TCCTCGTCCA TGGTGAAGGG CTCCTTCTTA AAGTTAAACA AGGCGGCCGC GGAGCCTGCT
  901 TTTTTGTACA AACTTGTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG
  961 CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC
 1021 CACTTGATGT TGACGTTGTA GCGCCGGCAG CTGCACGGGC TCTGGCTTGT AGTGTCTGGA
 1081 CTCAGCGTCG TATGATGCGG TCTTCAGCTT CAGCTCTGCT TGGATCTCGC CCATCAGGAC
1141 GCCGATCCCT CGGGGGTAC
//
Primer: CMV-Forward ()
Sequence file: RDB14699_A7Djc.seq check
>D04483A3_A7Dj_1_CMV-Forward_C05_08_ABI24.ab1
    1 ACTAATCAAC TTTCCGCCCC AATTGACGCA AATGGGCGGT AGGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT
  181 GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA
  241 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC
  301 CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC
  361 CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC
  421 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT
  481 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA
  541 GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA
  601 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT
  661 GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT
  721 CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT
  781 CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC
  841 CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGAAGCTTA CAAGTTTGTA
  901 CAAAAAAGCA GGCTCCGCGG CCGCCTTGTT TAACTTTAAG AAGGAGCCCT TCACCATGGA
  961 CGAGGACGTG CTAACCACCC TGAAGATCCT CATCATCGGC GAGAGTGGGG TGGGGCAAGT
 1021 CCAGCCCTGC TCTTGAGGTC ACAGATGATA CGGTTTGATC CAGACTGCAG CACAATAGGT
 1081 GTGACTTAGG TGAAACAATT TTCATGATGA ATTAGGCCTT AACTGCAATA TGGGATAACC
1141 TGCCTGGGTC AAGAAGAGGA GAAGT
//
Primer: SV40pro_F ()
Sequence file: RDB14699_A7Djd.seq check
>D04483A3_A7Dj_1_SV40pro_F_A01_01_ABI24.ab1
    1 CCCCGTCCTT ACTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCCGC CCTTCTATGA AAGGTTGGGC
 1021 TTTCGGGAAT CGTTTTCCGG GGACGCCCGG GCTGGGATGA TCCTCCAGCG CGGGGATCTC
 1081 ATGCTGCAGT CTTCGCCCAC CCCCAAACTG GTTATGGCCA GCCTATATTG TTACAATTAA
1141 GCAGTAGCCA TCAACAAATT TTCGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]