Please review the QC test results indicated by icon below as well as clone information before placing your order.

mKeima/pcDNA3

Plasmid clone of monomeric Keima-Red (mKeima).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18368_B0Gkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB18368_B0Gka.seq
>D04498L1_B0Gk_1_CMV_Forward_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCC NTTGNNNCNN ATGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT   121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CCATGGTGAG   181 TGTGATCGCT AAACAAATGA CCTACAAGGT TTATATGTCA GGCACGGTCA ATGGACACTA   241 CTTTGAGGTC GAAGGCGATG GAAAAGGAAA GCCTTACGAG GGAGAGCAGA CAGTAAAGCT   301 CACTGTCACC AAGGGTGGAC CTCTGCCATT TGCTTGGGAT ATTTTATCAC CACAGCTTCA   361 GTACGGAAGC ATACCATTCA CCAAGTACCC TGAAGACATC CCTGATTATT TCAAGCAGTC   421 ATTCCCTGAG GGATATACAT GGGAGAGGAG CATGAACTTT GAAGATGGTG CAGTGTGTAC   481 TGTCAGCAAT GATTCCAGCA TCCAAGGCAA CTGTTTCATC TACAATGTCA AAATCTCTGG   541 TGAGAACTTT CCTCCCAATG GACCTGTTAT GCAGAAGAAG ACACAGGGCT GGGAACCCAG   601 CACTGAGCGT CTCTTTGCAC GAGATGGAAT GCTGATAGGA AACGATTATA TGGCTCTGAA   661 GTTGGAAGGA GGTGGTCACT ATTTGTGTGA ATTTAAATCT ACTTACNAGG CAAAGAAGCC   721 TGTGAGGATG CNNGGGCGCC ACGAGATTGA CCGCAAACTG GATGTAANCA GTCACAACAG   781 GGATTACACA TCTGTTGAGC AGTGTGAAAT ANCCNTTGCA CGCCACTCTT TGCTCGGTTG   841 ANAATTCTGC ANATATCCAT CACACTGGNG GCCGCTCGAN CATGNNTCTA GANGNNCCTA   901 TTCTATAGTG NCNNCTNAAT GCTNNANCTC GCTGNNCNGC CNCNNCTGNG CCTTCTANNN 961 NNNAGNCNTC TGNNGNTTNN NNNTCCCCNN NGNNNNNNNN GANCNNNNNN NNNN //
Primer: BGH_rev3 (Pr0885) Region: BGH p(A), insert 3' Sequence file: RDB18368_B0Gkb.seq
>D04498L1_B0Gk_1_bGH_rev3_G07_19_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TCTAGATGCA   121 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTCAACCGAG CAAAGAGTGG   181 CGTGCAATGG CTATTTCACA CTGCTCAACA GATGTGTAAT CCCTGTTGTG ACTGGTTACA   241 TCCAGTTTGC GGTCAATCTC GTGGCGCCCT GGCATCCTCA CAGGCTTCTT TGCCTTGTAA   301 GTAGATTTAA ATTCACACAA ATAGTGACCA CCTCCTTCCA ACTTCAGAGC CATATAATCG   361 TTTCCTATCA GCATTCCATC TCGTGCAAAG AGACGCTCAG TGCTGGGTTC CCAGCCCTGT   421 GTCTTCTTCT GCATAACAGG TCCATTGGGA GGAAAGTTCT CACCAGAGAT TTTGACATTG   481 TAGATGAAAC AGTTGCCTTG GATGCTGGAA TCATTGCTGA CAGTACACAC TGCACCATCT   541 TCAAAGTTCA TGCTCCTCTC CCATGTATAT CCCTCAGGGA ATGACTGCTT GAAATAATCA   601 GGGATGTCTT CAGGGTACTT GGTGAATGGT ATGCTTCCGT ACTGAAGCTG TGGTGATAAA   661 ATATCCCAAG CAAATGGCAG AGGTCCACCC TTGGTGACAG TGAGCTTTAC TGTCTGCTCT   721 CCCTCGTAAG GCTTTCCTTT TCCATCGCCT TCGACCTCAA AGTAGTGTCC ATTGACCGTG   781 CCTGACATAT AAACCTTGTA NGTCATTTGT TTAGCGATCA CACTCACCAT GGTGGATCCG   841 ANCTCGGTAC CAAGCTTGGG TCTCCNTATA GTGAGTCGTA TTANTTTCNA TAANCCAGTA   901 AGCAGTGGGT TCNCNAGTTA NCCAGANANC NCTGCTNATA TAGANCNCCN ACCGNANACG   961 CCNACCGCCN ATTTNCGTCN NNGGGGCNGN ANTTGNNANN ANNTTTTNGA AAGNCCNNNN 1021 GANTTTNGNN GNNNAANNNA NNNNCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR Sequence file: RDB18368_B0Gkc.seq
>D04498L1_B0Gk_1_SV40pro_ori_F_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNC GCCTCTGCCT CTGANCTNTT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG   181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG   241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA   301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC   361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG   421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG   481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA   541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG   601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC   661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG   721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG   781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACNTAGCGTT GGCTANCCGN GATATTGCTG   841 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGNGCT TTACNGNATC GCCGCTCCCG   901 ATTCNNAGCG CATCGCCTTC TATCGCCNTC TTGANNANTN CNTCTGANCG GGNNTCNGGG   961 NNNCNAANNG ACCGANCNNG CGACGCCCNA NCNNGNNNTC NCNANNTTTC NNNTNNNNCN 1021 NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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