zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.
Clone info. | zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus. |
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Comment | Refer Fukuhara, S. et al., Dev. Biol. 393 (1): 10-23, 2014. |
Vector backbone | pT2KXIGdeltain (plasmid) |
Size of vector backbone | 8.1 kb |
Selectable markers | Amp^r |
Growth conditions | LB + Amp, 37oC |
Gene/insert name | zebrafish zGeminin cDNA Aequorea victoria GFP cDNA |
Reference of insert sequence | AB971707 (DDBJ accession number) |
Depositor|Developer | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49), 20812-20817 (2009). Additional terms and conditions: The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49), 20812-20817 (2009). 付加的使用条件: Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。 |
備考 | 提供条件は英文のまま記載してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15279 | mVenus-zGeminin (1/100) / pT2KXIGdeltain | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The mVenus-zGeminin (1/100) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15279). |
Reference section:
Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP] |
Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15279_A7H9p1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: beta-gi_F () Region: insert 5' Sequence file: RDB15279_A7H9a.seq ![]() |
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>D04498J1_A7H9_2_beta-gi_F_H06_08_ABI08.ab1 1 CAGGATTCAT GCCTTCTTCT CTTTCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG 61 TCTCATCATT TTGGCAAAGA ATTCCTCGAC GGATCCACTA GTAACGGCCG CCAGTGTGCT 121 GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA 181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC 241 CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG 301 GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA 361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC 421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA 481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT 541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA 601 GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA 661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA 721 CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA 781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA 841 CAAGGGATAT CCATCACACT GGCGGCCGCT CGAGATGAGT TCCATCAGAA GACCAAAGAA 901 TGCAGAAAAC CCGTCTGAAA ACATCAAGAA ATTCCTGGTA GCGCCAACAT CTGGAGCAGG 961 AATGGGGAGG AGGACGCTTC AGTGCTTCAG CCCTCTGCTG TAAACAAAAA CCTTGGTCGT 1021 ATAATTGAGA ATGGTAAGCA GTGGCCAAAA GGAAGATGTG AGCGCCGAGC AAGTTAAAGG 1081 TTCAAAGAAG AGTCAAAGCA GGAGGTGCCT GTCCAATCCA CAATGCAGGA AATGGAAAAC 1141 AGCCCCTGGA AGGGGGGTCT CACCAACCCA // |
Primer: pAxCALNL_F1 () Region: insert 3' Sequence file: RDB15279_A7H9b.seq ![]() |
>D04498J1_A7H9_2_pAxCALNL_F1_F07_16_ABI24.ab1 1 CTCCAGTGGT TTCTTAATCA ATGTCTGGAT CATCAATCGA TTACTCATAG GCCTCTTGTG 61 TGACCCCCTC AGGCTGGTTT TCATTTTCTG CATTTGTGGA TTTGACAGCC ACCTCTGCTT 121 TGACTCTCTT TGAACCCTTA ACTTGCTCGG CGCTCCACAT CTTCCTTTTG GCCACTGCTT 181 TACCATTCTC AATTATACGA CCAAGGTTTT TGTTTACAGC AGAGGGCTGA AGCACCTGAA 241 GCGTCCTCCT CCCCATTCCT GCTCCAGATG TTGGCGCTAC CAGGAATTTC TTGATGTTTT 301 CAGACGGGTT TTCTGCATTC TTTGGTCTTC TGATGGAACT CATCTCGAGC GGCCGCCAGT 361 GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA 421 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC TTGGACTGGT 481 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT 541 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG 601 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT 661 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA 721 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT 781 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA 841 AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCACGCCG TAGCCCAGGG 901 TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGGTGCAGAT CAGCTTCAGG 961 GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGGCC GGGACACGCC TGAACTTGGT 1021 GGGCCGTTTA CCGTCCGCCG GTCCAGCTCG AACCAAGGAT GGGGCACCCA CCCCCCGGAT 1081 GAAACAGGCT CTCGGCCCGT TGCTCCACCA ATGGATTCCA AGCACCAACT TGGCGGCCGT 1141 TAACCTATAG GTGGGAATCT CCCGAGTC // |
Primer: EF1a-N_R2 () Region: Tol2 R Sequence file: RDB15279_A7H9c.seq ![]() |
>D04498J1_A7H9_2_EF1a-N_R2_G07_19_ABI24.ab1 1 CTTAACGGGC CAAAGAATTG CAAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG 61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC 121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC 181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA 241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA 301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG 361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT 421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC 481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA 541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA 601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT 661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG 721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG 781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA 841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGGTAG 901 ATGGACCGGG GGAGGCTGAT GAGTTCTTAG CCCAGCGCTC AAAGATGCTA GAAATGATGT 961 AAAGAAACAA ATATGCAGAT GAAAGTTTAA GATAAGATAA AAAATTTAAA CAAATAAATG 1021 CAATTTATGG TTTTGTGTTT CTATTCCACC CAAATAATAA CCTCAACGGC AGTAAAGCCC 1081 ACCAGGATAA TTTTTTGTCC ATGCTCTGCA AGGCATCCGA ACTCTTAGCA TGAATCTCTC 1141 CA // |
Primer: pAxEF_R2 () Region: Tol2 L Sequence file: RDB15279_A7H9d.seq ![]() |
>D04498J1_A7H9_2_pAxEF_R2_H07_22_ABI24.ab1 1 TGGGGAAAAA ATGCCTTTTT ATTTTGGTGA AATTTGTGGA TGCTATTGCT TTAATTTGTA 61 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 121 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG 181 GCTGATTATG ATCCTCTAGA TCAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT 241 GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT 301 TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT 361 GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC 421 ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT 481 CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA 541 AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA 601 AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA 661 AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC 721 CTCTGGTTCT TGACCCCCTA CCTTCAGCAA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG 781 CCGCCAACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTTA GTGAGGGTTA ATTGCGCGCT 841 TGGCGTAATC ATGGTCATAA GCTGGTTTCC TGTGTGAAAT TGTTAATCCG CTCACAATTT 901 CCACACAACA TACGAAGCCG GAAGGCATAA AGTGTAAGCC CTGGGTGCCT AAATGAGTGA 961 GCTAAACTCA CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAAGTC GGGAAAACCT 1021 GTTCGTGGCC AAGCTGCATT AAATGAAATT CGGGCCAACG CGCGGGGGGA AGAAGGCGGT 1081 TTGCGTAATG GGCGCTCCTT TCCGGCATTC CCTCGCTTCA CTTGACCTCC CCTGCCGCCC 1141 TCGGCTCGAT TCGGACCTGG CGGGGCGAAG ACGCG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP] |
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original | Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP] |
reference | Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130. |
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