Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mVenus-zGeminin (1/100) / pT2KXIGdeltain

zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.

Catalog number RDB15279
Resource name mVenus-zGeminin (1/100) / pT2KXIGdeltain
Clone info. zGem-based S/G2/M marker for zebrafish cells to label nuclei with yellow fluorescent protein mVenus.
Comment Refer Fukuhara, S. et al., Dev. Biol. 393 (1): 10-23, 2014.
Vector backbone pT2KXIGdeltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name zebrafish zGeminin cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AB971707 (DDBJ accession number)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
zebrafish zGeminin Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA., 106 (49), 20812-20817.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15279 mVenus-zGeminin (1/100) / pT2KXIGdeltain DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mVenus-zGeminin (1/100) / pT2KXIGdeltain was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15279).

          Reference section:

          Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP]
          Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Transposon and plasmid, Kawakami laboratory (English text)
          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15279_A7H9p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: beta-gi_F ()
          Region: insert 5'
          Sequence file: RDB15279_A7H9a.seq check
          >D04498J1_A7H9_2_beta-gi_F_H06_08_ABI08.ab1
              1 CAGGATTCAT GCCTTCTTCT CTTTCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG
             61 TCTCATCATT TTGGCAAAGA ATTCCTCGAC GGATCCACTA GTAACGGCCG CCAGTGTGCT
            121 GGAATTCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
            181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
            241 CACCTACGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
            301 GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA
            361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
            421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
            481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
            541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA
            601 GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA
            661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
            721 CAACCACTAC CTGAGCTACC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
            781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
            841 CAAGGGATAT CCATCACACT GGCGGCCGCT CGAGATGAGT TCCATCAGAA GACCAAAGAA
            901 TGCAGAAAAC CCGTCTGAAA ACATCAAGAA ATTCCTGGTA GCGCCAACAT CTGGAGCAGG
            961 AATGGGGAGG AGGACGCTTC AGTGCTTCAG CCCTCTGCTG TAAACAAAAA CCTTGGTCGT
           1021 ATAATTGAGA ATGGTAAGCA GTGGCCAAAA GGAAGATGTG AGCGCCGAGC AAGTTAAAGG
           1081 TTCAAAGAAG AGTCAAAGCA GGAGGTGCCT GTCCAATCCA CAATGCAGGA AATGGAAAAC
          1141 AGCCCCTGGA AGGGGGGTCT CACCAACCCA
          //
          Primer: pAxCALNL_F1 ()
          Region: insert 3'
          Sequence file: RDB15279_A7H9b.seq check
          >D04498J1_A7H9_2_pAxCALNL_F1_F07_16_ABI24.ab1
              1 CTCCAGTGGT TTCTTAATCA ATGTCTGGAT CATCAATCGA TTACTCATAG GCCTCTTGTG
             61 TGACCCCCTC AGGCTGGTTT TCATTTTCTG CATTTGTGGA TTTGACAGCC ACCTCTGCTT
            121 TGACTCTCTT TGAACCCTTA ACTTGCTCGG CGCTCCACAT CTTCCTTTTG GCCACTGCTT
            181 TACCATTCTC AATTATACGA CCAAGGTTTT TGTTTACAGC AGAGGGCTGA AGCACCTGAA
            241 GCGTCCTCCT CCCCATTCCT GCTCCAGATG TTGGCGCTAC CAGGAATTTC TTGATGTTTT
            301 CAGACGGGTT TTCTGCATTC TTTGGTCTTC TGATGGAACT CATCTCGAGC GGCCGCCAGT
            361 GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA
            421 ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGC TTGGACTGGT
            481 AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT
            541 GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG
            601 CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGTAGTTGT
            661 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA
            721 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT
            781 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA
            841 AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCACGCCG TAGCCCAGGG
            901 TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGGTGCAGAT CAGCTTCAGG
            961 GGTCAGCTTG CCGTAGGTGG CATCGCCCTC GCCCTCGGCC GGGACACGCC TGAACTTGGT
           1021 GGGCCGTTTA CCGTCCGCCG GTCCAGCTCG AACCAAGGAT GGGGCACCCA CCCCCCGGAT
           1081 GAAACAGGCT CTCGGCCCGT TGCTCCACCA ATGGATTCCA AGCACCAACT TGGCGGCCGT
          1141 TAACCTATAG GTGGGAATCT CCCGAGTC
          //
          Primer: EF1a-N_R2 ()
          Region: Tol2 R
          Sequence file: RDB15279_A7H9c.seq check
          >D04498J1_A7H9_2_EF1a-N_R2_G07_19_ABI24.ab1
              1 CTTAACGGGC CAAAGAATTG CAAGTTTGAT TTGCATTGGA AAGGGTCGCT GGCTTTTGTG
             61 TTACACGCCC CTTATTTGTG CTTGATTAGA TGATCCCCCT GCTCGAGGGC CCATCTGGCC
            121 TGTGTTTCAG ACACCAGGGA GTCTCTGCTC ACGTTTCCTG CTATTTGCAG CCTCTCTATC
            181 AAGACTAATA CACCTCTTCC CGCATCGGCT GCCTGTGAGA GGCTTTTCAG CACTGCAGGA
            241 TTGCTTTTCA GCCCCAAAAG AGCTAGGCTT GACACTAACA ATTTTGAGAA TCAGCTTCTA
            301 CTGAAGTTAA ATCTGAGGTT TTACAACTTT GAGTAGCGTG TACTGGCATT AGATTGTCTG
            361 TCTTATAGTT TGATAATTAA ATACAAACAG TTCTAAAGCA GGATAAAACC TTGTATGCAT
            421 TTCATTTAAT GTTTTTTGAG ATTAAAAGCT TAAACAAGAA TCTCTAGTTT TCTTTCTTGC
            481 TTTTACTTTT ACTTCCTTAA TACTCAAGTA CAATTTTAAT GGAGTACTTT TTTACTTTTA
            541 CTCAAGTAAG ATTCTAGCCA GATACTTTTA CTTTTAATTG AGTAAAATTT TCCCTAAGTA
            601 CTTGTACTTT CACTTGAGTA AAATTTTTGA GTACTTTTTA CACCTCTGTC AAGAACTCCT
            661 GGACAAACCT CTGACCTGTG TGGAACAGAG TGGATATGGG TGTCTGAACA GATATTCACG
            721 TCTTTTGCAG ATCAGAGGGC ATTTCTGGTG TTGTGCAATG ACCTGGGTCC AACAGGTAGG
            781 CGTACTCGTA TCCAAGAGCA TTGTTGGACA GGAGGTAGTC TCCATTCCTA TAAAGAGGAA
            841 GGAATGGCAC CATGTAGTAG CCGTCATTGT GGCCAATTGG TGCATTGGTA CGTGGGGTAG
            901 ATGGACCGGG GGAGGCTGAT GAGTTCTTAG CCCAGCGCTC AAAGATGCTA GAAATGATGT
            961 AAAGAAACAA ATATGCAGAT GAAAGTTTAA GATAAGATAA AAAATTTAAA CAAATAAATG
           1021 CAATTTATGG TTTTGTGTTT CTATTCCACC CAAATAATAA CCTCAACGGC AGTAAAGCCC
           1081 ACCAGGATAA TTTTTTGTCC ATGCTCTGCA AGGCATCCGA ACTCTTAGCA TGAATCTCTC
          1141 CA
          //
          Primer: pAxEF_R2 ()
          Region: Tol2 L
          Sequence file: RDB15279_A7H9d.seq check
          >D04498J1_A7H9_2_pAxEF_R2_H07_22_ABI24.ab1
              1 TGGGGAAAAA ATGCCTTTTT ATTTTGGTGA AATTTGTGGA TGCTATTGCT TTAATTTGTA
             61 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
            121 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
            181 GCTGATTATG ATCCTCTAGA TCAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT
            241 GTGGCTGATT TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT
            301 TCTTCCACTG ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT
            361 GGTAATAGAT GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC
            421 ATATAATGTG AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT
            481 CAGCGCAATT CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA
            541 AATAAGTACT TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA
            601 AAAAATGTAA TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA
            661 AATCCCCAAA AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC
            721 CTCTGGTTCT TGACCCCCTA CCTTCAGCAA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG
            781 CCGCCAACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTTA GTGAGGGTTA ATTGCGCGCT
            841 TGGCGTAATC ATGGTCATAA GCTGGTTTCC TGTGTGAAAT TGTTAATCCG CTCACAATTT
            901 CCACACAACA TACGAAGCCG GAAGGCATAA AGTGTAAGCC CTGGGTGCCT AAATGAGTGA
            961 GCTAAACTCA CATTAAATTG CGTTGCGCTC ACTGCCCGCT TTTCCAAGTC GGGAAAACCT
           1021 GTTCGTGGCC AAGCTGCATT AAATGAAATT CGGGCCAACG CGCGGGGGGA AGAAGGCGGT
           1081 TTGCGTAATG GGCGCTCCTT TCCGGCATTC CCTCGCTTCA CTTGACCTCC CCTGCCGCCC
          1141 TCGGCTCGAT TCGGACCTGG CGGGGCGAAG ACGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuhara, S., Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 393 (1): 10-23 (2014). PMID 24975012. [link to RRC of NBRP]
          original Sugiyama, M., Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. U.S.A. 106 (49): 20812-20817 (2009). PMID 19923430. [link to RRC of NBRP]
          reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

          2023.11.23

          GNP_filter3_RDBDEP_html_231027.pl