Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBaitD-gap43-linker-EGFP

Donor plasmid for targeted insertion into the gap43 locus of Japanese medaka by the BaitD system.

Sequence (full)	RDB15409zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Murakami, Y. et al. Zool. Letters 3: 10, 2017).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Murakami, Y. et al. Zool. Letters 3: 10, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15409_A7Ikp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_C_F (Pr0271) Region: AmpR,AmpR ter,Bait7,GAP43 intron1,exon2,linker Sequence file: RDB15409_A7Ikd.seq
>D04849A1_A7lk_1_Amp_C_F_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTC GNANTTANCT ACNCGACGGG GNNTCAGGCA ACTATGGATG    61 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG   121 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA   181 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGAGCGGATA CACGTAGTGG ATCTTCGGCC   241 TAGACTGCGA GGCTCGAGGA ACATCTCAGT CTAAGACTCC ATTTTTCTTA GCAAATGTAC   301 ATTATAGCTA CAAAAGGTTC CTCTGGACCC ACATGACTGC TGCTAGCAGC TAATATTCTG   361 TACTGTGTGT TGTTCATCAG GTGGAGAAAA ATGAGGATGC AGACCAGAAG ATTGAGCAGG   421 ATGGCACCGC CAACAAACCC GAGGACAACA AGGCCCACAA GGCAGCCACC AAAATACAGG   481 CTAGCTTCCG TGGACACATA ACTCGGAAAA AGATGAAAGA CGGAGAGGAG GAGAAGGAAG   541 AAAACAGTCC TGTTGCAGAT GAGGCAAAAG AGAGAGAAGA GGAGACAAAG AAAGCAGAGG   601 GAGATGAGAC ACCTGCTAAG CAGGATGAAG CTGCAGGAGA GGAGGCGAAG GAAGAGTTAA   661 ACCAAGCCAA AAGCCCTGCA GCAGACAAAC CAGCTAACTC TCCGGCAGCT GCTGTCTCTC   721 CTGCAGGCGC AGCCACTTCT CCCGGATCCG GANGTAGCGG TGGAACTATG GTGAGCAAGG   781 GCGAGGANCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA NCTGGACGGC GACGTAAACG   841 GCCACAAGTT CAGCGTGTCC GGCGNNGGCG ANGNCGANGC CANCNACGGC AAGCTGANCC   901 TGAANTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCNN GCCNACCNNC NNGNNCNCCN   961 NNNNCNACNG NNNGCANNGN TNNAGCNGNN ACCCNNNNCN NNNNNAGCAN CNNNNNNNNN 1021 N //
Primer: SV40pA3element Reverse (Pr0108) Region: Bait7,GAP43 intron3,exon3,intron2,insert seq,exon2,SV40 pA Sequence file: RDB15409_A7Ike.seq
>D04849A1_A7lk_1_SV40pA3'ElementReverse_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNGNNNNN NTGGNNANNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG    61 CCTTTTGCTG GCCTTTTGCT GATCTTCGGC CTAGACTGCG AGGACTAGTC CTTATAAATG   121 CTTCACTATA CACTTACCAC ATGCACGACA AAGGTTTGTT CCATTTTCAT AAAGTCCCTT   181 AAGTTGGTTG TAGGACCCTA AAGGAAACTG CAAGACACAC CTGCTCTCCC CTTAGGATGC   241 AGCCACTCTT CTCTATGACC CTCTATGGGC CCGGATAACC AAAAAAACTG TAGCACCCAT   301 ACCGGTCATG TGACCAAGGC TTAACTGGAG CGCTGTAATC GGCATACCTT GTTGATCTTT   361 TGTTTGGTTA GGTTCTTCCT CAGCTGTTGG GCTGACAGCA GCAGGCACAT CGGCTTGTCT   421 GGCCTCCTCC TTCTTCTCCT CCCACTTTTT CTCCTCCTCC TTCTTCTCCT CAGCTGTGGC   481 TGTGGTGGGA CTCTTGGTTG TTGCAGCTGG AGCCTCCACC TCTTTGGGCT TCTCCTTCGG   541 CTCCTCTTTC TCAGGTTCAG AGGGCGCCGT GGCAGTTGTA GGAGCAGATG GAGCCGGCGC   601 CACAGGAATT CTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA   661 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG   721 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGANGT   781 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACNAATGT GGTATGGCTG ATTATGATCT   841 AGANTCGCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG ANAGTGATNC CGGCGGCGGT   901 CNNGAACTCC AGCAGGNNCA TGTGATNGCG CTTCNCGTTG GGGTCTTTNC TCNNGGCNNN 961 NNNGGNGNNC NNNNANNGGN TNNNCNGGNA NCNNCNNNGG GGNNNNNNNN NN //
Primer: pGCAP_R (Pr0074) Region: GAP43 exon2,insert seq,intron2,exon3,intron3 Sequence file: RDB15409_A7Ikf.seq
>15409_15409_B2DdF_1_pGCAP_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN CCGGCTCCNT CTGCTCCTAC NANCTGCCAC    61 GGCGCCCTCT GAACCTGAGA AAGAGGAGCC GAAGGAGAAG CCCAAAGAGG TGGAGGCTCC   121 AGCTGCAACA ACCAAGAGTC CCACCACAGC CACAGCTGAG GAGAAGAAGG AGGAGGAGAA   181 AAAGTGGGAG GAGAAGAAGG AGGAGGCCAG ACAAGCCGAT GTGCCTGCTG CTGTCAGCCC   241 AACAGCTGAG GAAGAACCTA ACCAAACAAA AGATCAACAA GGTATGCCGA TTACAGCGCT   301 CCAGTTAAGC CTTGGTCACA TGACCGGTAT GGGTGCTACA GTTTTTTTGG TTATCCGGGC   361 CCATAGAGGG TCATAGAGAA GAGTGGCTGC ATCCTAAGGG GAGAGCAGGT GTGTCTTGCA   421 GTTTCCTTTA GGGTCCTACA ACCAACTTAA GGGACTTTAT GAAAATGGAA CAAACCTTTG   481 TCGTGCATGT GGTAAGTGTA TAGTGAAGCA TTTATAAGGA CTANNCCTCG CAGTCTAGGC   541 CGAAGATCAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG   601 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG   661 TGGCGAANCC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG   721 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA   781 AGCGTGGCGC TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGNGNA NGNTCGTTCG   841 CTNCAAGCTG GGCTGTGTGC ACGANCCCCC CGTNNANCCC GANCGCTGCN CCTTNTCCNG   901 GNAACTATCN NNNNGAGTCN NANCCNGNNN ANNANNNNAN NTNNTCGCCN CNNGGCNNCN   961 NCCNNCNGGN NANNNNGNNN NNNNNANCNA GGNATGNNNG GNGGNGNNNN NNNANTTNNT  1021 TGNANNGGNG GNNNNNNCNN NCNGNTACNN NNNNANNNAN NNNNNNTTTN GNNNNCNGNN 1081 CTNNNNNGNA NNNCNNNNNN NNCNNNNNGA AAANNANNNN GNNNNNNNNN N //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',SV40 pA,GAP43 exon2,insert seq Sequence file: RDB15409_A7Ikg.seq
>15409_15409_B2DdF_1_mVenus-M_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGTGAACT TNNAGANCCG CCNCNACATC    61 GAGGANGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC   121 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC   181 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC   241 GGCATGGACG AGCTGTACAA GTAAAGCGGC CGCGACTCTA GATCATAATC AGCCATACCA   301 CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC   361 ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT   421 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG   481 GTTTGTCCAA ACTCATCAAT GTATCTTAGA ATTCCTGTGG CGCCGGCTCC ATCTGCTCCT   541 ACAACTGCCA CGGCGCCCTC TGAACCTGAG AAAGAGGAGC CGAAGGAGAA GCCCAAAGAG   601 GTGGAGGCTC CAGCTGCAAC AACCAAGAGT CCCACNNNNN NCACAGCTGA GGAGAAGAAN   661 NNNNNGGAGA AAAAGTGGGA GGAGAAGAAG GAGGAGGCCA GACAAGCCGA TGTGCCTGCT   721 GCTGTCAGCC CAACAGCTGA GGAAGAACCT AACCNAACNA AAGATCAACA AGGTATGCCG   781 ATTACAGCGC TCCAGNTTAA ANCCTTGGTC ANNNGACCGG TATGGGTGCN ACAGNTTTTT   841 TTNGGTTATC CGGGCCCNNT AGAGGGTCNT AGANNANAGT GGNNTGCNNN CCTAANGNGA   901 NAGCNNGTGN GNNNTNGNAG TTTTCCNTNA NGNNCCTACN ANCAACTTNA NGGNANTTTN   961 NNAAAATGGA NNAANNNNTT TGNCNNGNNT GTGNNAAGTG NNNTAGTGAN NCATTNNNNN 1021 AGGNCTANNC NTNNNNNNNN NN //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',linker,GAP43 exon2 Sequence file: RDB15409_A7Ikh.seq
>15409_15409_B2DdF_1_EGFP-M_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNGNNGGNN CTTGAAGTTN NCCTTGATGC CGTTCTTCTG    61 CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG   121 GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC   181 GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT   241 GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG   301 GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCAGG GTGGTCACGA GGGTGGGCCA   361 GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC   421 ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC   481 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TAGTTCCACC   541 GCTACCTCCG GATCCGGGAG AAGTGGCTGC GCCTGCAGGA GAGACAGCAG CTGCCGGAGA   601 GTTAGCTGGT TTGTCTGCTG CAGGGCTTTT GGCTTGGTTT AACTCTTCCT TCGCCTCCTC   661 TCCTGCAGCT TCATCCTGCT TAGCAGGTGT CTCATCTCCC TCTGCTTTCT TTGTCTCCTC   721 TTCTCTCTCT TTTGCCTCAT CTGCAACANG ACTGTTTTCT TCCTTCTCCT CCTCTCCGTC   781 TTTCATCTTT TTCCGAGTTA TGTGTNNNNN GNNNNTAGCC TGTATTTTGG TGGCTGCCTT   841 GTGGNCCTTG TTGTCCTCGG GTTTGTTGNN GGNNGCCNNN NTGCTCNNTN NTTCTGGTCT   901 GCANNCTCAN TTTTCTNCNN CNGNANGAAC AACACACAGT ACANANNANT AGCTGNTAGC   961 ANCANNCNTG NGGGNTNCAN ANGAACNNTT TNGNNNNNTA TNNNGNNNNN TTTGCNNNNN  1021 AAAAATGNAA NTNTNNNANN NNANNNGTTN CNTCNNNNNT CGNNNNNNAN NNNNNNNANN 1081 NNNNNNNN //
Primer: FP-N_F1 (Pr0920) Region: EGFP 3',SV40 pA Sequence file: RDB15409_A7Iki.seq
>15409_15409_B2DdF_1_FP-N_F1_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCN TGAAGCAGCA CGACTTCTNN NNNNCCGCCA    61 TGCCCGAAGG CTACNNNNNN NANCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA   121 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA   181 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC   241 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC   301 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA   361 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA   421 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG   481 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT AGATCATAAT   541 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT   601 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTNAC TTGTTTATTG NAGCTTATAA   661 TGGTTACNAA TAAAGCAATA GCATCACAAA NTTNNCAAAT AAAGNATTTT TTTNNNCNNN 721 NNNNNANNNN NNNNTNNNNN NANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl