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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIP-FLAG-Mafk (#RDB15412)

Expression vector of mouse Mafk.

Clone info. Expression vector of mouse musculoaponeurotic fibrosarcoma (MAF) oncogene family protein K (Mafk), tagged with FLAG at N-terminal, SV40 ori, IRES-puromycin resistance, CAG promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Please refer Niwa, H. et al., Nat. Genet. 24 (4): 372-376, 2000 for pCAGIP vector.
Vector backbone pCAGIP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Mouse Mafk cDNA
Depositor|Developer Kato, Mitsuyasu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Okita, Y et al. Sci. Signal. 10(474) pii: eaak9397, 2017)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15412 pCAGIP-FLAG-Mafk DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIP-FLAG-Mafk was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15412).

Reference section:

Okita, Y., Kimura, M., Xie, R., Chen, C., Shen, L.T., Kojima, Y., Suzuki, H., Muratani, M., Saitoh, M., Semba, K., Heldin, C.H., Kato, M., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15412_A7J2p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330)
Region: CAG pro,FLAG,insert5'
Sequence file: RDB15412_A7J2a.seq check
>D04907A1_A7J2_1_pCAG-F_V2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NNNNTCTGGG NNGNGCCGCC GCACCCCCTC
   61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
  121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG
  181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG
  241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
  301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCAGAT CCACCGCCAT
  361 GGACTACAAG GACGATGATG ACAAGGGCGA ATTCACGACT AATCCCAAGC CCAACAAGGC
  421 ATTGAAGGTC AAGAAGGAGG CGGGTGAGAA CGCCCCTGTT CTTAGCGATG ATGAGCTGGT
  481 GTCCATGTCA GTGCGGGAGC TAAACCAGCA CCTGCGGGGG CTCACCAAGG AGGAGGTCAC
  541 TCGGCTGAAG CAGCGGCGGC GCACACTCAA GAACAGAGGC TACGCGGCTA GCTGTCGCAT
  601 CAAGCGTGTG ACACAGAAAG AGGAGCTGGA ACGGCAGCGT GTGGAGCTAC AGCAGGAGGT
  661 GGAGAAGCTG GCTCGAGAGA ACAGCAGCAT GCGGCTGGAG CTAGATGCCC TGCGCTCCAA
  721 GTATGAGGCC CTACAGACCT TCGCTCGCAC CGTGGCCCGA NGGCCTGTCA CACCCACCNA
  781 GGTGGCCACC ACCAGTGTCA TCACCATCGT CAAATCTGCC GAGCTCTCCT CCNCCTCTGT
  841 ACCCTTCTCA GCCGCCTCCN ANTCNAGANG GNCCCCCCCT CGANGNCNAN GAANTCCNCC
901 CCCCCCCCCN NNNCNNNNNN NNNN
//
Primer: IRES-R (Pr0037)
Region: IRES,insert3',FLAG,CAG pro
Sequence file: RDB15412_A7J2b.seq check
>D04907A1_A7J2_1_IRES-R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT NAGGGGGGGG GGNNGGANAG GGGGGGGGGG
   61 CGGAATTCCT CGACCTCGAG GGGGGGCCCT CTAGACTAGG AGGCGGCTGA GAAGGGTACA
  121 GAGGTGGAGG AGAGCTCGGC AGATTTGACG ATGGTGATGA CACTGGTGGT GGCCACCTTG
  181 GTGGGTGTGA CAGGCCCTCG GGCCACGGTG CGAGCGAAGG TCTGTAGGGC CTCATACTTG
  241 GAGCGCAGGG CATCTAGCTC CAGCCGCATG CTGCTGTTCT CTCGAGCCAG CTTCTCCACC
  301 TCCTGCTGTA GCTCCACACG CTGCCGTTCC AGCTCCTCTT TCTGTGTCAC ACGCTTGATG
  361 CGACAGCTAG CCGCGTAGCC TCTGTTCTTG AGTGTGCGCC GCCGCTGCTT CAGCCGAGTG
  421 ACCTCCTCCT TGGTGAGCCC CCGCAGGTGC TGGTTTAGCT CCCGCACTGA CATGGACACC
  481 AGCTCATCAT CGCTAAGAAC AGGGGCGTTC TCACCCGCCT CCTTCTTGAC CTTCAATGCC
  541 TTGTTGGGCT TGGGATTAGT CGTGAATTCG CCCTTGTCAT CATCGTCCTT GTAGTCCATG
  601 GCGGTGGATC TGAATTCTTT GCCAAAATGA TGANACAGCA CAATAACCAG CACGTTGCCC
  661 AGGAGCTGTA GGAAAAAGAA GAAGGCATGA ACATGGTTAG CAGAGGCTCT AGAGCCGCCG
  721 GTCACACGCC ANAAGCCGAA CCCCGCCCTG CCCCGTCCCC CCCGAANGCA GCCGTCCCCC
  781 CGCGGANAGC CCCGANGCTG GANAGGGAGA ANGGGANGGC GGCGCGGCGA CGCACGAANG
  841 CCCTCCCCGC CCATTTCCTT CCTGCCGGCG CCGCACCGCT TCGCCCCGCG CCNGCTANAG
  901 GGGGNGCGGC GGCGCCNNCC NNATTTNGGN TCCNCACANA TTTNGGNANA AAGGAAGTCC
  961 CTGCNCCCNN TCNNACNATT ACCATAAANN GNNANNGGNN GNNNNNCNNN GCGCNTCGNN
1021 NGNNNNCNGG NNCNNNNGGG NNNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,PuroR
Sequence file: RDB15412_A7J2c.seq check
>D04907A1_A7J2_1_IRES-F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGAGG TTANNAAACG TCTAGGCCCC CCGAACCNCG
   61 GGGACGTGGT TTTCCTTTGN NAAACACGAT GATAATATGG CCACAACCAT GACCGAGTAC
  121 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
  181 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
  241 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
  301 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
  361 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
  421 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
  481 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
  541 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
  601 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
  661 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC
  721 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCNTG CATCGATGAT CTAGAGCTCG
  781 CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT
  841 GCCTTCCTTG ACCCTGNAAN GTGCCNCTCC CACTGTCCTT TCCTAANNAA ATGAGGAANN
  901 TGCNTCGCAT TGTCTGANTA NGTGTCATTC TATTCTGGGG GGGNGGGGTG GGGGCANGAC
  961 ANCAAGGGGG ANGANTGGGN AAGNNAATAN CNNNNTGCTG GGGNNGCNGT GGGCNNNANN
1021 GNNNTNNNNA NNCNGNAAAN NNNNNGNAGC CNNNNNNNNN N
//
Primer: Amp_R (Pr0008)
Region: Polyoma Ori,SV40 poly(A),HCMVIEE
Sequence file: RDB15412_A7J2d.seq check
>D04907A1_A7J2_1_Amp_R_A04_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAGANTGT ATTTCCTGGA AATTAATGTT
   61 TATTCNATAA ACTGTGTATT CAGCTATATT CACATAGTGG TGAGGCTGAA ATGAGGCGGG
  121 AAGAGGCGGT TGGGGCTTAA TTATATCAAT TTGGGTGGCC CCACAGCGCC TCCAAGGCGC
  181 CAGTCCTGTT TTGACAAGTT GCCTCTGGAA GCCTCTCTTC TTTTTCTCCA GAGTAAGCGG
  241 AGGCCAGGGG CCCCCGGCCT CTGCTTAATA CTAAAAAAAA CAGCTGTTGT CATAGTAATG
  301 ATTGGGTGGA AACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA
  361 CACTGACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGC
  421 CCTCTGGAGG GCAGTTGCCT AGCAACTAAT TAAAAGAGGA TGTCGCACGG CCAGCTGCGG
  481 TCAGTTAGTC ACTTCCTGCT TAACTGACTT GACATTTTCT ATTTTAAGAG TCGGGAGGAA
  541 AATTACTGTG TTGGAGGCCC TCCGCCATCT TCTGAAGCTG AATCGAATTA ACTTGTTTAT
  601 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
  661 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
  721 GATCTGATAT CATCGTCGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
  781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG
  841 CCTGGCTGAN CGNCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGNA TGTTCCCNTA
  901 GTAACGCCNA TANGGACTTN NNATTGACGT CAANNGGNGG AGTATTTANG GNAAACTGCC
  961 NNCTTGGNAN TANNTCAANN GTATCATATG CNNNGNANNN CCCCNANNGN NNNNNNTGAN
1021 NGNNAANNNN NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Okita, Y., The transcription factor MAFK induces EMT and malignant progression of triple-negative breast cancer cells through its target GPNMB. Sci. Signal 10 (474). pii: eaak9397 (2017). PMID 28400538. [link to RRC of NBRP]