Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(CA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(1/100)Cy(-)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15447_B1K5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(mCherry 5') Sequence file: RDB15447_B1K5g.seq
>D05018D2_B1K5_1_EF1a-C_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNTNNNNNNN NNNNNNTTTG GNNCTTGGTT NNTTCTCNAG CCTCAGACAG    61 TGGTTCAAAG TTTTTTTCTT CCNTTTCAGG TGTCGTGAAC ACGCTACCGG TCTCGAGAAT   121 TCTGCAGCGG CCGCGGATCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA   181 TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG   241 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG   301 TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG   361 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC   421 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA   481 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA   541 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG   601 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA   661 AGGACGGCGG CCACTACGAC GCTGNNGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC   721 AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT   781 ACNCCATCGT GNAACAGTAC GAACGCGCCG ANGGCCGCCA CTCCACCGGC GGCATGGACG   841 ANCTNNACAA GGGATATCCA TCACACNNGC GGCCGCTCGA GATGGNNCAG CGCCGCNNCA   901 NCGANNTCTN NGCGNGCNGC CGCCCCNGNN NCNCCNNNNT CGNGNCNCCN NNNNNNGNNN 961 TNNNNNANCC CNNNNNNNNN N //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(hGem(1/110),AmCyan1 3') Sequence file: RDB15447_B1K5h.seq
>D05018D2_B1K5_1_PRE_rev_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNAA ANGGNNCACN TAGTTAAGAN TACCAGTCAA TCTTTCNCAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGATT   121 ACAGCGCCTT TCTCCGTTTT TCTGCCACTT CCTTCCAATA CTGAGAGGAT GGATTTTCTT   181 TAATCATAAG ATCAAATGAC TCCTGGGTGA CTCCTCCAAG ATTTTTATTT TCACTAGATT   241 CTGGGACAAT AACCCCAGGG CTGGAAGTTG TAGATGTTAA GTGGTCATTC CGATGTTTCC   301 TTTTGGACAA GCCTGCGGAC AGCTCATTTT CTCTTCCAAC AAGAGATCCA GATGCAGAAG   361 GCTGAATCAT CTTCAGAGTT CTTCTTGGGA CAGAACTATT CTTTATATTC TCTTTGATTT   421 CTTCTTGTTT CTGCTTCATA CTGGGATTCA TCTCGAGCGG CCGCCAGTGT GATGGATATC   481 CGAAGGGCAC CACGGAGGTG ATGTGGGCCA CGGCGTGCTC GGTCAGCTGC ACGCTGTTGC   541 CGCCCTTGTC CAGGTCGGTT CTGGCGATGC GGTGCTCCAC CACGTGGTTG GGGGGCATGG   601 TCACGGGCTT CTTGGTCTTG TAGGAGGTGT GGAACTGGCA TCTGTAGTTG CCGCCGCCCT   661 GCAGCATCAG GAAGGCGGTC ACGTCGCCCT TCAAGATGCC GTCGCACACG GTCATCTTCT   721 CGAANGAGGG GTCCCAGCCG GTGGTCTTCT TGGNCATCAC GGGGCCGTCN GCGGGGAAGT   781 TCACGCCGTG GAAGGTGGAC TTGTGCTCGA AGCAGTTGNC CTTCNNNTGA TCTCCCAGCT   841 GGCGGNGGCC ACGCCGCCNN CCTCGTANGT GAAGGTTCTC TCGNANGACA TGCCNNCGGG   901 GAANGNCTGC TNGANNNAGN NNGNNNTGCT GGNGGGGNAN GNNNGNNGNN NCANCNGNNN 961 NNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(mCherry 3',hCdt1(1/100)Cy(-),P2A,AmCyan1 5') Sequence file: RDB15447_B1K5i.seq
>D05018D2_B1K5_1_mCherry-C_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CTGCNNGGNG CCTACNACGT CAACATCAAG TTGGACATCA    61 CCTCCCACAA CGNGGNNTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT   121 CCACCGGCGG CATGGACGAG CTGTACAAGG GATATCCATC ACACTGGCGG CCGCTCGAGA   181 TGGAGCAGCG CCGCGTCACC GACTTCTTCG CGCGCCGCCG CCCCGGGCCC CCCCGCATCG   241 CGCCGCCCAA GCTGGCCTGC CGCACCCCCA GCCCCGCCAG GCCCGCACTC CGCGCCCCGG   301 CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC GGACGCGACC   361 AGGCCAGGCC ACCGGCCCGC GCGGCCGCGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA   421 GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG ATAAAGAAAA   481 CCGGTCGCGC CAAGCGCGGC AGCGGCGCCA CCAACTTCAG CCTGCTGAAG CAGGCCGGCG   541 ACGTGGAGGA GAACCCCGGC CCCCGCGAAT TCGCCACCAT GGCCCTGTCC AACAAGTTCA   601 TCGGCGACGA CATGAAGATG ACCTACCACA TGGACGGCTG CGTGAACGGC CACTACTTCA   661 CCGTGAAGGG CGAGGGCAGC GGCAAGCCCT ACGAGGGCAC CCAGACCTCC ACCTTCAAGG   721 TGACCATGGC CAACGGCGGC CCCCTGGCCT TCTCCTTCGA CATCCTGTCC ACCGTGTTCA   781 TGTACGGCAA CCGCTGCTTC ACCGCCTACC CCACCAGCAT GCCCGACTAC TTCAAGCNNG   841 NCTTCCCCGA CGGCATGTCC TACNAGAGAA NCTTCACCTA CNNNGNNGGC GGCGNGGNCA   901 CCGCCAGCTG GGNNATCANC CNGNANNGGN NNCNGNNTCN NNNACNNGNN NNNCNTTNCN 961 NNNGNNNNNN NNNTNNCNNN NCNNNNNGNN CCNNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB15447_B1K5j.seq
>D05018D2_B1K5_1_CMV_Forward_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGNNGCNN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG   121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT   181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC   241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA   301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA   361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG   421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA   481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC   541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA   601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA   661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG   721 GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACNAAAGTA AGACCACCGC ACANCAAGGG   781 CCGCTGATCT TCNGACCTGG NNGANGANAT ATGAGGGACA ATTNGAGAAN TGAATTATAT   841 AAATATNAAA GTAGTAAAAA TTGANNCATT NNGANTAGCN CCNACNNAGG CAAANANAAG   901 NNNGGNGCAG AGAGAAAAAA GANCANTNNN NNTANGNNCT TTGTTNCTTN GGNTCNNGGG 961 NNNNNNCNNN NNNNNNN //
Primer: BGH_rev3 (Pr0885) Region: 3'LTR,PRE Sequence file: RDB15447_B1K5k.seq
>D05018D2_B1K5_1_bGH_rev3_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNGNNGGNNN NNNACNGANG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG NTCNNCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA   121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG   181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC   241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC   301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC   361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC   421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA   481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA   541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC   601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG   661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA   721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG   781 ACGGTATCGA TGCGGGGNGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAN GGCGAANGCG   841 AANANGCGGA ANANGNCGCA NANCCGGCAG CNNGCCGCGG GAANGAANNN CCGCNNGNNT   901 NNNGGNCGAA NGNNNGTANC ANANNNNNNT CCNNNGNNNN NNNCANGNGG NNNCNCNNNN 961 NNNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo,SV40 pA Sequence file: RDB15447_B1K5l.seq
>D05018D2_B1K5_1_SV40pro_F_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNANNANTGN NGNNGCTTTT TTGGNNGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG NATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT   121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG   181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT   241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG   301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA   361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG   421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT   481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG   541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG   601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC   661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA   721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT   781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC NACCTCTAGC TAGAGCTTGN   841 CGTANTCATG GNCATAGCTG TTTCCTGTGN NAAATTGTTA TCCGCTCACN ATNNCNCNCA   901 ACATACNNNC CGGAAGCATA AAGNGNAAAG CCNNGGGGNN NNTNNNGAGN GAGCNNNNNC 961 NNNNNNANNN NNNNTNNNNN NNNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mCherry 3',hCdt1(1/100)Cy(-),P2A) Sequence file: RDB15447_B1K5m.seq
>D05018D2_B1K5_1_mRFP1-inner1_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNTCC TCCNTGCAGG NNGGCGNNTT CNTCTACNAG    61 GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG   121 GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC   181 AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC   241 AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC   301 ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC   361 TCCACCGGCG GCATGGACGA GCTGTACAAG GGATATCCAT CACACTGGCG GCCGCTCGAG   421 ATGGAGCAGC GCCGCGTCAC CGACTTCTTC GCGCGCCGCC GCCCCGGGCC CCCCCGCATC   481 GCGCCGCCCA AGCTGGCCTG CCGCACCCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG   541 GCCTCCGCTA CCAGTGGCAG CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC   601 CAGGCCAGGC CACCGGCCCG CGCGGCCGCG CGGCTGTCGG TGGACGAGGT TTCCAGCCCC   661 AGTACCCCCG AGGCCCCAGA CATCCCAGCC TGCCCTTCTC CGGGCCAGAA GATAAAGAAA   721 ACCGGTCGCG CCAAGCGCGG CAGCGGCGCC ACCAACTTCA GCCTGCTGAA GCAGGCCGGC   781 GNCGTGGANG ANAANNCCGG CCCCCGCGAA TTCGCCACCA TGGCCCTGTC CNACAAGTTC   841 ATCGGCGANG ACATGAAGAN GANCNACCNC ATGGNNNGNN GCGNGAACNG NCNCNACTNN 901 ANNNNGNANN GGNGAGGGNA GNGGNNNNNN NTACNANGGC NCCCANNNNN NNNNN //
Primer: AmCyan1-H_F (Pr1071) Region: insert mid(AmCyan1 3',hGem(1/110) 5') Sequence file: RDB15447_B1K5n.seq
>D05018D2_B1K5_1_AmCyan1-H_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNNNNNN CTCCNCCTNN NNNGTGNNCA TGGCCAACGG    61 CGGCCCCCTG GNCTTCTCCT TCGACATCCT GTCCACCGTG TTCATGTACG GCAACCGCTG   121 CTTCACCGCC TACCCCACCA GCATGCCCGA CTACTTCAAG CAGGCCTTCC CCGACGGCAT   181 GTCCTACGAG AGAACCTTCA CCTACGAGGA CGGCGGCGTG GCCACCGCCA GCTGGGAGAT   241 CAGCCTGAAG GGCAACTGCT TCGAGCACAA GTCCACCTTC CACGGCGTGA ACTTCCCCGC   301 CGACGGCCCC GTGATGGCCA AGAAGACCAC CGGCTGGGAC CCCTCCTTCG AGAAGATGAC   361 CGTGTGCGAC GGCATCTTGA AGGGCGACGT GACCGCCTTC CTGATGCTGC AGGGCGGCGG   421 CAACTACAGA TGCCAGTTCC ACACCTCCTA CAAGACCAAG AAGCCCGTGA CCATGCCCCC   481 CAACCACGTG GTGGAGCACC GCATCGCCAG AACCGACCTG GACAAGGGCG GCAACAGCGT   541 GCAGCTGACC GAGCACGCCG TGGCCCACAT CACCTCCGTG GTGCCCTTCG GATATCCATC   601 ACACTGGCGG CCGCTCGAGA TGAATCCCAG TATGAAGCAG AAACAAGAAG AAATCAAAGA   661 GAATATAAAG AATAGTTCTG TCCCNAGAAG AACTCTGAAG ATGATTCAGC CTTCTGCATC   721 TGGATCTCTT GTTGGAAGAG AAAATGAGCT GTCCGCAGGC TTGTCCAAAA GGAAACATCG   781 GANTGACCAC TTAACATCTA CAACTTCCAG CCCTGGGGTT ATTGTCCCAG AATCTAGTGA   841 AAATAAAAAT CTTGGAGGAN TCANCCAGGA NTCATTTGAT CTTATGANNN AAGAAAANNC   901 NTNCTNTCAG NATNNGNNNN GNANTGGNAG AAAAANNGAN AANGGNGCTN NNNNNTANNN 961 NTNNNNNNNN NN //
Primer: EF1a-N_R (Pr0024) Region: cPPT_CTS,RRE Sequence file: RDB15447_B1K5o.seq
>D05018D2_B1K5_1_EF1a-N_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGCGNNGT GCGCTCTGCC CACTGNCGGG CACCGGNNCC    61 TCACGAAGCT TGNATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT   121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT   181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA   241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT   301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC   361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA   421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC   481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT   541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG   601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA   661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG   721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT   781 GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA NANGCTGTTG CGCCTCAATA   841 GCCCTCANCA AATTGTTCTG CTGCTGCACT ATACCAGANA ATAATTGTCT GGNCNGNANC   901 NNCAGCGNCA TTGNNGCTGC GCCCATANNG NTNNCNGCTG NTCCCNNNNA NNCCNNNNNN 961 NNAANNNNCN NANTNNCNNN NNNNNNTTTT TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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