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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-FIP1L1-PDGFRA-FL

Expression vector of human FIP1L1-PDGFRA (full-length), FLAG-tag.

Catalog number RDB15460
Resource name pFLAG-FIP1L1-PDGFRA-FL
Clone info. Expression vector of human FIP1L1-PDGFRA (full-length), tagged with FLAG at N-terminal.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pFLAG-CMV (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human FIP1L1 cDNA human PDGFRA cDNA
Depositor|Developer Kondo, Takeshi |
Other clones in our bank

External Database
human FIP1L1 human PDGFRA

        Reference sequence
          
         
        Sequence (full) RDB15460zzk02.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ibata, M et al. Cancer Sci. 108 (2), 200-207, 2017)。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB15460 pFLAG-FIP1L1-PDGFRA-FL DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pFLAG-FIP1L1-PDGFRA-FL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15460).

        Reference section:

        Ibata, M., Iwasaki, J., Fujioka, Y., Nakagawa, K., Darmanin, S., Onozawa, M., Hashimoto, D., Ohba, Y., Hatakeyama, S., Teshima, T., Kondo, T., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.

        Further references such as user reports and related articles (go to bottom)


        References and tips

        Featured content


        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB15460_B2Cgp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Sequence file: RDB15460_B2Cga.seq check
        >D04855A7_B2Cg_3_ CMV_Forward_A04_01_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGNNGCNAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
           61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTAATTC AGATCGATCT ACCATGGACT
          121 ACAAAGACGA TGACGACAAG CTTGCGGCCG CGAATTCATC GATAGATCTG ATATCGGTAC
          181 CAGTCGACTC TAGAGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG GAGCTGAGCG
          241 GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG CGATGAAAAT GAAGTTGAAA
          301 GGCCAGAAGA AGAAAATGCC AGTGCTAATC CTCCATCTGG AATTGAAGAT GAAACTGCTG
          361 AAAATGGTGT ACCAAAACCG AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG
          421 ATGATGATGA AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT
          481 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA AGAGTTTATG
          541 GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC ACCTGGAAGC ATTAATGGAG
          601 TTCCACTCTT AGAGGTAGAT TTGGATTCTT TTGAAGATAA ACCATGGCGT AAACCTGGTG
          661 CTGATCTTTC TGATTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG
          721 AAAAACAAAA GAGGANACGA ATGGGACTTG AAGTTATACC AGTAACCTCT ACTACNAATA
          781 AAATTACNGT ACAGCANGGA AGANNTGGAA ACTCANANAA AGAAACTGNC CTTCCNTCTA
          841 CAAAAGCTGA NTTNACTTCT CCTNCTTCTT TGTTCNANAN TGGGCTTCCN CCNANCNNNA
          901 NANTACNNGG GGNNATNGAT GNNNNCGGNC ANANTNTNAC TATCANCCNA NTANANNNNN
        961 NNNGANNGNN NANTGANNNN NGCNNCNTAC NGCTGNNNNN NNNNN
        //
        Primer: PDGFRA_F2
        Sequence file: RDB15460_B2Cgb.seq check
        >D04855A7_B2Cg_3_ PDGFRA_F2_F04_16_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CCTCCTTTTA CCNCCNNGAG TGANNNNNNN
           61 NAGAATCTNG CTGCCNNGGA CAATATAAAA AGAGTTATGA AAAAATTCAC CTGGACTTCC
          121 TGAAGAGTGA CCATCCTGCT GTGGCACGCA TGCGTGTGGA CTCAGACAAT GCATACATTG
          181 GTGTCACCTA CAAAAACGAG GAAGACAAGC TGAAGGACTG GGAGGGTGGT CTGGATGAGC
          241 AGAGACTGAG CGCTGACAGT GGCTACATCA TTCCTCTGCC TGACATTGAC CCTGTCCCTG
          301 AGGAGGAGGA CCTGGGCAAG AGGAACAGAC ACAGCTCGCA GACCTCTGAA GAGAGTGCCA
          361 TTGAGACGGG TTCCAGCAGT TCCACCTTCA TCAAGAGAGA GGACGAGACC ATTGAAGACA
          421 TCGACATGAT GGACGACATC GGCATAGACT CTTCAGACCT GGTGGAAGAC AGCTTCCTGT
          481 AACTCGACCT GATGGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG
          541 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA
          601 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA
          661 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG NCTGCGGGGT CTATTGGGAA NNNAGCTGGA
          721 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGNNTTCAA GCGATTCTCC
          781 TGCCTCAGCC TCCCGAGTTG TTGGGANTCC AGGNATGCNT GANNNNGCTC ANCTAANTTT
          841 TGTTTTTTTG GNANANNNGG GGTTTCACCA TATTGGCNNN GCTNNNCTCN NACTNCNNAN
          901 CTCNNGNGAN NNACCCANCT NGNNNNCCCN AANNGNNGGN NTNCNNGNNT GNNCNANNGC
        961 TCCNNNCCNN TNNTTNNNAN TTNAAANNAN NNNNNNNNN
        //
        Primer: FIP1L1_F1
        Sequence file: RDB15460_B2Cgc.seq check
        >D04855A7_B2Cg_3_ FIP1L1_F1_C04_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNTCTT TTGNNGNNAA ACCATGGCGT
           61 AAACCTGGTG CTGATCTTTC TGATTATTTT AATTATGGGT TTAATGAAGA TACCTGGAAA
          121 GCTTACTGTG AAAAACAAAA GAGGATACGA ATGGGACTTG AAGTTATACC AGTAACCTCT
          181 ACTACAAATA AAATTACGGT ACAGCAGGGA AGAACTGGAA ACTCAGAGAA AGAAACTGCC
          241 CTTCCATCTA CAAAAGCTGA GTTTACTTCT CCTCCTTCTT TGTTCAAGAC TGGGCTTCCA
          301 CCGAGCAGGA GATTACCTGG GGCAATTGAT GTTATCGGTC AGACTATAAC TATCAGCCGA
          361 GTAGAAGGCA GGCGACGGGC AAATGAGAAC AGCAACATAC AGCTGCCATA TGACTCAAGA
          421 TGGGAGTTTC CAAGAGATGG ACTAGTGCTT GGTCGGGTCT TGGGGTCTGG AGCGTTTGGG
          481 AAGGTGGTTG AAGGAACAGC CTATGGATTA AGCCGGTCCC AACCTGTCAT GAAAGTTGCA
          541 GTGAAGATGC TAAAACCCAC GGCCAGATCC AGTGAAAAAC AAGCTCTCAT GTCTGAACTG
          601 AGGATAATGA CTCACCTGGG GCCACATTTG AACATCGTAA ACTTGCTGGG AGCCTGCACC
          661 AAGTCAGGCC CCATTTACAT CATCACAGAG TATTGCTTCT ATGGAGATTT GGTCAACTAT
          721 TTGCATAAGA ATAGGGANAG CTTCCTGAGC CANCACCCAG AGAANCCAAA GAAAGAGCTG
          781 GATATCTTTG GACTGAACCC TGCTGATGAA AGCACACGGA NCTATGTTAT TTTATCTTTT
          841 GAAAACAATG GTGANTACNT GGACNTGAAG CNNNTGATAC TACNCANNAT GNNCCCNNGC
          901 TANAAAGGAA ANANGNTNCT AAANATTCCN ANNTNCNNAN NTNANTCNAT GATCGTCCNN
          961 CNTCNTNNNN ANNAANTCTA TGNNANNNTN NNNANTCNAA ANNNNNNNTT CNNNNGNNNN
        1021 NNNNNN
        //
        Primer: FIP1L1v3_F2
        Sequence file: RDB15460_B2Cgd.seq check
        >D04855A7_B2Cg_3_FIP1L1v3_F2_G04_19_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GCGNNNGNNN NNNAGAACNG CAACATACAG CTGCCATATG
           61 ACTCAAGATG GGAGTTTCCA AGAGATGGAC TAGTGCTTGG TCGGGTCTTG GGGTCTGGAG
          121 CGTTTGGGAA GGTGGTTGAA GGAACAGCCT ATGGATTAAG CCGGTCCCAA CCTGTCATGA
          181 AAGTTGCAGT GAAGATGCTA AAACCCACGG CCAGATCCAG TGAAAAACAA GCTCTCATGT
          241 CTGAACTGAG GATAATGACT CACCTGGGGC CACATTTGAA CATCGTAAAC TTGCTGGGAG
          301 CCTGCACCAA GTCAGGCCCC ATTTACATCA TCACAGAGTA TTGCTTCTAT GGAGATTTGG
          361 TCAACTATTT GCATAAGAAT AGGGATAGCT TCCTGAGCCA CCACCCAGAG AAGCCAAAGA
          421 AAGAGCTGGA TATCTTTGGA CTGAACCCTG CTGATGAAAG CACACGGAGC TATGTTATTT
          481 TATCTTTTGA AAACAATGGT GACTACATGG ACATGAAGCA GGCTGATACT ACACAGTATG
          541 TCCCCATGCT AGAAAGGAAA GAGGTTTCTA AATATTCCGA CATCCAGAGA TCACTCTATG
          601 ATCGTCCAGC CTCATATAAG AAGAAATCTA TGTTAGACTC AGAAGTCAAA AACCTCCTTT
          661 CANGTGATAA CTCAGAANGN CTTACTTTAT TGGATTTGTT GAGCTTCNCC TATCAAGTTG
          721 CCCGAGGAAT GGAGTTTTTG GCTTCNAAAA NTTGTGTCCA CCGNGATCTG GCTGCTCGCA
          781 ACGTCCTCCT GGCACAAGGA AAAANTGTGA ANANCTGTGA NTTTNNNATN NNNNGANANA
          841 TCATGNNTGA NTCGAANTAT NNGNCNNNAN NNAGTANCTT TCTGNCNNNG AANTGNNNGG
          901 NTNNNGNNAN CNTNTTNNNN NNNNNCNACN NNNCNCNNNN NGNNGNNNGG NNTNANNNNN
        961 NNNNN
        //
        Primer: PDGFRA_F1
        Sequence file: RDB15460_B2Cge.seq check
        >D04855A7_B2Cg_3_ PDGFRA_F1_E04_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN CNNGAAGGNN NAGGNTTCNN AAATATTCCG ACATCCAGAG
           61 ATCACTCTAT GATCGTCCAG CCTCATATAA GAAGAAATCT ATGTTAGACT CAGAAGTCAA
          121 AAACCTCCTT TCAGGTGATA ACTCAGAAGG CCTTACTTTA TTGGATTTGT TGAGCTTCAC
          181 CTATCAAGTT GCCCGAGGAA TGGAGTTTTT GGCTTCAAAA AATTGTGTCC ACCGTGATCT
          241 GGCTGCTCGC AACGTCCTCC TGGCACAAGG AAAAATTGTG AAGATCTGTG ACTTTGGCAT
          301 GGCCAGAGAC ATCATGCATG ATTCGAACTA TGTGTCGGAA GGCAGTACCT TTCTGCCCGT
          361 GAAGTGGATG GCTCCTGAGA GCATCTTTGA CAACCTCTAC ACCACACTGA GTGATGTCTG
          421 GTCTTATGGC ATTCTGCTCT GGGAGATCTT TTCCCTTGGT GGCACCCCTT ACCCCGGCAT
          481 GATGGTGGAT TCTACTTTCT ACAATAAGAT CAAGAGTGGG TACCGGATGG CCAAGCCTGA
          541 CCACGCTACC AGTGAAGTCT ACGAGATCAT GGTGAAATGC TGGAACAGTG AGCCGGAGAA
          601 GAGACCCTCC TTTTACCACC TGAGTGAGAT TGTGGAGAAT CTGCTGCCTG GACAATATAA
          661 AAAGAGTTAT GAAAAAATTC ACCTGGACTT CCTGAAGAGT GACCATCCTG CTGTGGCACG
          721 CATGCGTGTG GACTCAGACA ATGCATACAT TGGTGTCACC TACNAAAACG AGGAANANAA
          781 GCTGAANGAN TGNNNNGGTG GTCTGGATGA NCAGANANTG ANCGCTGANA GTGNCTACNT
          841 CATTCCTCTG NCTGANNNTG ANCCNGNCCC NGANGANGAN GANNNGGGCA AGANGAACAN
          901 NNNCNNCTCG CANANCTCTG AANANAGTGN CNTNGANANN GNNNCCNNCA NTNCCNNCTT
        961 CNTCNNNANA NANNNNNANA NNNTTNNNNA NNTCNNNNTG ANNNNNNANN NNNNNNNN
        //
        Primer: SV40pro_ori_F
        Sequence file: RDB15460_B2Cgf.seq check
        >D04855A7_B2Cg_3_ SV40pro_ori_F_D04_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNGNNG NCNCCTCGGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
           61 CTTTTTTGGA GGAATTGATC AGCTTGGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
          121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
          181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
          241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
          301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
          361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
          421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
          481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
          541 GCATCGAGCG AGCACGTACT CNNNTGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
          601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
          661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
          721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGN TGTGGCGGAN CGCTATCAGG
          781 ACATAGCGTT GGCTACCCGT GATATTGCTG AANAGCTNGN CGGCGAATGG GCTGANCGCT
          841 TCCNCGNGNT TTACNGNATC GCCGCTNCCN ATTCNNANCG NATCNCCNTC TATCNNCNNN
          901 NNGANNANTN CTTCTGANNG GNNNTCNGGG GNNCNAAATG ANNGANCNAN CNNNNNCCNN
        961 NCNNNNNNTN NNNANATTTC NANTCCNNNN NNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ibata, M., Leukemogenic kinase FIP1L1-PDGFRA and a small ubiquitin-like modifier E3 ligase, PIAS1, form a positive cross-talk through their enzymatic activities. Cancer Sci. 108 (2): 200-207 (2017). PMID 27960034.
        reference Iwasaki, J., FIP1L1 presence in FIP1L1-RARA or FIP1L1-PDGFRA differentially contributes to the pathogenesis of distinct types of leukemia. Ann. Hematol. 93 (9): 1473-1481 (2014). PMID 24763514.

        2023.05.01

        GNP_filter3_RDBDEP_html_230501.pl