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pEGFP-C1-mouse Rab5A-Q79L

Expression vector of mouse Rab5A constitutive active mutant EGFP-tag.

Catalog number RDB15726
Resource name pEGFP-C1-mouse Rab5A-Q79L
Clone info. Expression vector of mouse Rab5A constitutive active mutant (GTP-hydrolysis-deficient, Q79L), fused with EGFP at N-terminal. CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope. Please refer Itoh, T. et al., Genes. Cells, 11 (9): 1023-1037, 2006 (PMID 16923123) for gene cloning.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^R (mammalian cells).
Gene/insert name mouse Rab5a cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rab5a

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. Genes Cells 11, 1023-1037, 2006); (Ishida, M. et al. J. Cell Sci. 125, 5177-5187, 2012)。
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          RDB15726 pEGFP-C1-mouse Rab5A-Q79L DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15726_A8A9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP, insert 5'
          Sequence file: RDB15726_A8A9a.seq check
          >D05154A3_A8A9_1_EGFP-C2_E10_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNCGNNC NCNTGGTCCT GCTGGANTTC GTGACCGCCG CCGGGNTCAC
             61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTAATC GAGGAGCAAC
            121 AAGACCCAAC GGGCCAAATA CTGGAAATAA AATATGCCAG TTCAAACTGG TCCTTCTAGG
            181 AGAGTCTGCT GTTGGCAAAT CAAGCCTGGT TCTTCGCTTT GTGAAAGGCC AATTTCATGA
            241 ATTTCAAGAG AGTACCATTG GGGCTGCCTT TCTAACCCAA ACTGTGTGTC TTGATGACAC
            301 AACAGTAAAA TTTGAAATAT GGGATACAGC TGGTCTCGAG CGGTATCATA GCTTAGCACC
            361 AATGTACTAC CGAGGAGCAC AAGCAGCCAT AGTTGTGTAT GATATCACAA ATGAGGAATC
            421 CTTTGCGAGA GCAAAAAACT GGGTTAAAGA ACTTCAAAGG CAAGCAAGTC CTAATATTGT
            481 GATAGCTTTG TCAGGAAACA AAGCTGACTT AGCAAATAAA AGAGCTGTTG ACTTCCAGGA
            541 AGCACAGTCC TATGCAGATG ACAACAGCTT ATTATTTATG GAGACATCAG CTAAGACATC
            601 AATGAATGTA AATGAAATAT TTATGGCAAT AGCTAAAAAG CTGCCAAAGA ATGAACCACA
            661 GAATCCTGGT GCAAACTCAG CCAGAGGANG AGGAGTAGAC CTTACTGAGC CTGCACAGCC
            721 AGCCAGAAGC CAGTGTTGTA GTAACTGAAA TCGAATAATC GAATTCTGCA GTCGACGGTA
            781 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCNGCCNT ACCACATTTG
            841 TAGANGNTTT ACTTGCTTTN AAAAACCTCC CNNACCNNCC CCNNAANCTG AAANATNAAA
            901 TGAANGNNAT TGNNGNTGNT NNCTTGNTTA NNNNANCNTN NTAANGGNNN NNANTAAAGC
          961 NNNANCNTCN NNANNTTNNN NANNNANNNN NTTTTTTNNN NNNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: insert 3'
          Sequence file: RDB15726_A8A9b.seq check
          >D05154A3_A8A9_1_SV40polyA-R2_F10_16_ABI24.ab1
              1 NNNNNNNNNN NCNNNNNNNN NNNGTTAACA ANNACAATTG CATTCNTTTT ATGTTTCAGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
            181 GAATTCGATT ATTCGATTTC AGTTACTACA ACACTGGCTT CTGGCTGGCT GTGCAGGCTC
            241 AGTAAGGTCT ACTCCTCGTC CTCTGGCTGA GTTTGCACCA GGATTCTGTG GTTCATTCTT
            301 TGGCAGCTTT TTAGCTATTG CCATAAATAT TTCATTTACA TTCATTGATG TCTTAGCTGA
            361 TGTCTCCATA AATAATAAGC TGTTGTCATC TGCATAGGAC TGTGCTTCCT GGAAGTCAAC
            421 AGCTCTTTTA TTTGCTAAGT CAGCTTTGTT TCCTGACAAA GCTATCACAA TATTAGGACT
            481 TGCTTGCCTT TGAAGTTCTT TAACCCAGTT TTTTGCTCTC GCAAAGGATT CCTCATTTGT
            541 GATATCATAC ACAACTATGG CTGCTTGTGC TCCTCGGTAG TACATTGGTG CTAAGCTATG
            601 ATACCGCTCG AGACCAGCTG TATCCCATAT TTCAAATTTT ACTGTTGTGT CATCAAGACA
            661 CACAGTTTGG GTTAGAAAGG CAGCCCCAAT GGTACTCTCT TGAAATTCAT GAAATTGGCC
            721 TTTCACAAAG CGAAGAACCA GGCTTGATTT GCCAACAGCA GACTCTCCTA GAANGANCAG
            781 TTTGAACTGG CATATTTTAT TTCCAGTATT TGGCCCGTTG GGTCTTGTTG CTCCTCNATT
            841 ANCCATGGNT CTGANTCCGG ANTTGTACAG CTCGNCCATG NCGANANTGA TNCCGGCGNC
            901 GGNCACGANN TCCAGCAGGA NCATGTGATC GCGCTTNNCG TTGGGGNCTT TGCTNNNGNN
            961 GNANTGGGNG CTCNNNNANT GGNNNNCGGN NNNCANCNNG GGGNNNNNGN NNANGGGGGN
          1021 NNNCNGNNNN NNNNNGNNNN NNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, EGFP
          Sequence file: RDB15726_A8A9c.seq check
          >D05154A3_1_A8A9_CMV-Forward_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNTNGNNG CNNTGGGCGG TANGCGTGTA CGGTGGGANG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
            121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
            181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
            241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
            301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
            361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
            421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
            481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
            541 TACAACTACN ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
            601 GTGAACTTCA AGATCCGCNA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGANCACTAC
            661 CAGCAGAACA CNNNCATCGG CGACGGCCCC GNGCTGCTGC CCGACAACCA CTACCTGANC
            721 ACCCAGTCCG CCCTGANCAA AGANCCCNAC GAGAANCGCG ATCACATNGN CCTGCTNGAN
          781 TNCGTGANCG CCNCCNGGNT CANTCTCNNN NNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB15726_A8A9d.seq check
          >D05154A3_A9Kr_1_SV40pro_F_V2_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTTG NAAGNNCGNN NANGAGNNAG GNNGAGGNNC GTTTCGCATG
             61 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
            121 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
            181 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
            241 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
            301 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
            361 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
            421 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
            481 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
            541 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
            601 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
            661 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA
            721 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
            781 GTGCTTTACG GTATCGCCGC TCCNGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
            841 GAGTNCTTCT GANCGGGNNT CTGGGGTTCN NAATGNNNNA NCNAGCGACG CCCNANNNNN
          901 NNTCACNANN TTNCGANTCC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci. 125 (Pt 21): 5177-5187 (2012). PMID 22854043.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2023.04.06

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