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GFP-NLS PARP (#RDB15741)

Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm.

Clone info. Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm: expression vector of GFP, tagged with a single NLS sequence of poly (ADP-ribose) polymerase (PARP) at C-terminal.
Comment Expression was confirmed by the depositor with microscopic observation. Utilized EGFP with monomeric mutation (A206K, D.A. Zacharias et al., Science. 296 (5569): 913-916, 2002).
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kitamura, A. et al., Biochem. Biophys. Res. Commun., 463 (3): 401-406, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15741 GFP-NLS PARP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GFP-NLS PARP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15741).

Reference section:

Kitamura, A., Nakayama, Y., Kinjo, M., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15741_A8Anp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA_R2 ()
Region: Sv40p(A),PARP NLS,mEGFP
Sequence file: RDB15741_A8Ana.seq check
>D05001A3_A8An_4_SV40polyA-R2_C02_03_ABI08.ab1
    1 GCAAATTAAT AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG
  181 CAGAATTCGA AGCTTCGGCT TTCAGCGCTT TTTCCAGTTT GCTATCTTTA TCCTTTTCTT
  241 TCTTGCTCTT CTTTTTGGCC ACTTCGCCGC CTTTGCGTTT GCCTTCGCTT TTCACAGATC
  301 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
  361 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG GACTGGGTGC
  421 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT
  481 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT
  541 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT
  601 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC
  661 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT
  721 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT
  781 CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG
  841 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA
  901 GCTTGCGTAG TGGCATCGCC CTCGCCCTCG CCGGACAACG CTGAACTTGT GGCCGTTTAC
  961 CGTCGCCGTC CAACTCGACC AGGATGGGCA ACACCCCGGT GACAGCTCTC GCCCTTGGCT
 1021 CACCATGGTG GCGACCGGGT ACCGCTTACC GGATCTGTAC GGTTCACTAA ACCCAGCTCC
 1081 TGCTTATATA TGACCTCCCA CCCGTTCACG CCTAACCGCC CCAATTTGCT TTCAATGGGG
 1141 GCGGAAGTTG TTTCGAAACA TTTTGGAAGT CCCCGTTTGA TTTTGGGTGG CCAAAATCAA
1201 CCTTCCAATT GAACGTCTTA TGGGGGTTGG TAGAGCTTTG GGGAATACTC CGCCTCG
//
Primer: CMV_Forward ()
Region: CMV pro,mEGFP,PARP NLS
Sequence file: RDB15741_A8Anb.seq check
>D05001A3-4_A8An_CMV-Forward_G02_20_ABI24.ab1
    1 CATCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA
  841 TCTGTGAAAA GCGAAGGCAA ACGCAAAGGC GGCGAAGTGG CCAAAAAAGA AGAGCAAGAA
  901 AGAAAAGGAT AAAGATAGCA AACTGGGAAA AAGCGCTGAA AGCCGAAAGC TTCGAAATTC
  961 TGCAGTCGAC GGGTACCGCG GGGCCCGGGG ATCCCACCCG GGATCTAGAT AACTGATCAT
 1021 AATCAGCCCA TACCAACATT TTGGTAGAGG GTTTTACTTT GCTTTAAAAA AAAACTTCCC
 1081 ACACGTTCCC CTCCCTTGGA ACTTGCAAAT CCATTCAAAT GATGCTATTG CATGCCTGGG
1141 CTA
//
Primer: SV40pro_F_V2 ()
Region: NeoR_KanR
Sequence file: RDB15741_A8Anc.seq check
>D05001A3-4_A8An_SV40pro_F_V2_H02_23_ABI24.ab1
    1 GGGCAGTTTT TTGGAGGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCCTCCAGC GCGGGGGATC TCATGCTGGG
 1021 AGTTCTTTCG CCCACCCTAG GGGGGAGGCT ACTGGAAACA CGGAAGGAGA CCATTACCCG
1081 AAGACCCCGC CGCTATTGAC GGCCAATTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463 (3): 401-406 (2015). PMID 26032495. [link to RRC of NBRP]