Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmirGLO-TOP2A-3'-UTR (#RDB15800)

Reporter plasmid of human TOP2A-3'-UTR region for quantitatively evaluate miRNA activity.

Clone info. Reporter plasmid of human topoisomerase 2 alpha (TOP2A)-3'-UTR region for quantitatively evaluate miRNA activity.
Comment Expression was confirmed by the depositor with reporter assay.
Vector backbone pmirGLO (plasmid)
Size of vector backbone 7.4 kb
Selectable markers Amp^r
Gene/insert name human TOP2A cDNA
Depositor|Developer Kondo, Yutaka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15800 pmirGLO-TOP2A-3'-UTR DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmirGLO-TOP2A-3'-UTR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15800).

Reference section:

Deguchi, S., Katsushima, K., Hatanaka, A., Shinjo, K., Ohka, F., Wakabayashi, T., Zong, H., Natsume, A., Kondo, Y., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15800_A8Bqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: luc2-c_F (Pr0546)
Region: Luc 2 3',insert 5'
Sequence file: RDB15800_A8Bqa.seq check
>D05196A7-4_A8Bq_luc2-C_F_D02_2018-03-19-14-24-28_11.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNT CTCNTTAAGG CCNAGAAGGG CGGCAAGATC
   61 GCCGTGTAAT TCTAGTTGTT TAAACGAGCT CGCTAGCCTC GAGAATGTGA GGCGATTATT
  121 TTAAGTAATT ATCTTACCAA GCCCAAGACT GGTTTTAAAG TTACCTGAAG CTCTTAACTT
  181 CCTCCCCTCT GAATTTAGTT TGGGGAAGGT GTTTTTAGTA CAAGACATCA AAGTGAAGTA
  241 AAGCCCAAGT GTTCTTTAGC TTTTTATAAT ACTGTCTAAA TAGTGACCAT CTCATGGGCA
  301 TTGTTTTCTT CTCTGCTTTG TCTGTGTTTT GAGTCTGCTT TCTTTTGTCT TTAAAACCTG
  361 ATTTTTAAGT TCTTCTGAAC TGTAGAAATA GCTATCTGAT CACTTCAGCG TAAAGCAGTG
  421 TGTTTATTAA CCATCCACTA AGCTAAAACT AGAGCAGTTT GATTTAAAAG TGTCACTCTT
  481 CCTCCTTTTC TACTTTCAGT AGATATGAGA TAGAGCATAA TTATCTGTTT TATCTTAGTT
  541 TTATACATAA TTTACCATCA GATAGAACTT TATGGTTCTA GTACAGATAC TCTACTACAC
  601 TCAGCCTCTT ATGTGCCAAG TTTTTCTTTA AGCAATGAGA AATTGCTCAT GTTCTTCATC
  661 TTCTCAAATC ATCAGAGGCC GAAGAAAAAC ACTTTGGCTG TGTCTATAAC TTGACACAGT
  721 CAATAGAATG AAGAAAATTA GAGTAGTTAT GTGATTATTT CAGCTCTTGA CCTGTCCCCT
  781 CTGGCTGCCT CTGAGTCTGA ATCTCNCAAA GAGAGAAACC AATTTCTAAG ANGANTGGAT
  841 TGCAGAAGAN TCGGGGNNAA CATTTGATCC NAGATCTTAA ATGNTATATT GATAANNNTG
  901 CTCAGCAATG AGCTATTAGA NTCATTTTGG GAAATCTNNN TNNTTTCNNN TTGTAAACTT
961 NGNTNANNNN NGNCTANNTT NNNATATGNG NGNNNNTTNN NTNANNNNNN NNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,insert 3'
Sequence file: RDB15800_A8Bqb.seq check
>15800_15800_B0JdF_SV40pA_R_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT TNNNNAATAA AGCATTTTTT TCNCTGCATT CTAGTTGTGG
   61 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGCTCG AAGCGGCCGC CCCAAGGGGT
  121 TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG GGCTTTGTTA
  181 GCAGCCGGAT CAGCTTGCAT GCCTGCAGGT CGACTCTAGA TTCTATGGGT TGCAATGTTT
  241 AGAACATTTT ATTAAAGTAC AAAATTGTTG GAATTTAGCT AATAGAAAAA CATAGTAAAT
  301 ATTTACAAAA ACGTTGATAA CATTACTCAA GTCACACACA TATAACAATG TAGACAGGTC
  361 TTAACAAAGT TTACAAATTG AAATTATGGA GATTTCCCAA AATGAATCTA ATAGCTCATT
  421 GCTGAGCATG GTTATCAATA TAACATTTAA GATCTTGGAT CAAATGTTGT CCCCGAGTCT
  481 TCTGCAATCC AGTCCTCTTA GAAATTGGTT TCTCTCTTTG GGAGATTCAG ACTCAGAGGC
  541 AGCCAGAGGG GACAGGTCAA GAGCTGAAAT AATCACATAA CTACTCTAAT TTTCTTCATT
  601 CTATTGACTG TGTCAAGTTA TAGACACAGC CNAAGTGNTT TTCTTCGGCC TCTGATGATT
  661 TGAGAAGANG AANNANATGA GCAATTTCTN NTNGCTTNAA GAAAAANNNN NNNNNTNNNA
721 NNNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: hRluc 5'
Sequence file: RDB15800_A8Bqc.seq check
>D05196A7-4_A8Bq_SV40pro_F_G01_2018-03-19-14-24-28_19.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GNNGNNGNTT TTTTGGAGGC CTAGGCTTTT
   61 GNNNNNAGCT TGATTCTTCT GACNCNNNNN NCTCGAACCA AAGGCTGGAG CCACCATGGC
  121 TTCCAAGGTG TACGACCCCG AGCAACGCAA ACGCATGATC ACTGGGCCTC AGTGGTGGGC
  181 TCGCTGCAAG CAAATGAACG TGCTGGACTC CTTCATCAAC TACTATGATT CCGAGAAGCA
  241 CGCCGAGAAC GCCGTGATTT TTCTGCATGG TAACGCTGCC TCCAGCTACC TGTGGAGGCA
  301 CGTCGTGCCT CACATCGAGC CCGTGGCTAG ATGCATCATC CCTGATCTGA TCGGAATGGG
  361 TAAGTCCGGC AAGAGCGGGA ATGGCTCATA TCGCCTCCTG GATCACTACA AGTACCTCAC
  421 CGCTTGGTTC GAGCTGCTGA ACCTTCCAAA GAAAATCATC TTTGTGGGCC ACGACTGGGG
  481 GGCTTGTCTG GCCTTTCACT ACTCCTACGA GCACCAAGAC AAGATCAAGG CCATCGTCCA
  541 TGCTGAGAGT GTCGTGGACG TGATCGAGTC CTGGGACGAG TGGCCTGACA TCGAGGAGGA
  601 TATCGCCCTG ATCAAGAGCG AAGAGGGCGA GAAAATGGTG CTTGAGAATA ACTTCTTCGT
  661 CGAGACCATG CTCCCAAGCA AGATCATGCG GAAACTGGAG CCTGAGGAGT TCGCTGCCTA
  721 CCTGGAGCNN TTCAAGGAGA AGGGCGAGGT TAGACGGCCT ANCCTCTCCT GNCCTCGCGA
  781 NATCCCTCTC GTTAAGGGAN GCAAGCCCGA CGTCGTCCAG ATTGTCCGCA ACTACNACGN
  841 CTACCTTCGG GCCANCGNCG ATCTGCCTAA GATGTTCATC GANTCCGANC CNGGGTTCTT
  901 TTNCNNCNCT ATTGTCNAGG GNNCTNAGAN NTTCCCTAAC NNNNNNTTCG TGNNNNNGNN
961 NGNNNTNNNN TTCAGCNNNN NNNNNN
//
Primer: Neo-M_R (Pr0145)
Region: NeoR 5',hRluc 3'
Sequence file: RDB15800_A8Bqd.seq check
>D05196A7-4_A8Bq_Neo-M_R_A02_2018-03-19-14-24-28_02.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNNT TNNGTGACNA CGTCGAGCAC AGCTGCGCAA
   61 GGAACGCCCG TCGTGGCCNG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG
  121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC
  181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC
  241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCCCTCC GGAGCCTCCG
  301 CCACCTGATC CGCCACCTCC GCTCCCACCA CCGGTCTGCT CGTTCTTCAG CACGCGCTCC
  361 ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT GAAGTGGAGG
  421 CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC AATAGCGTTG
  481 GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT GGCCCGAAGG
  541 TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT AACGAGAGGG
  601 ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT GAATGGCTCC
  661 AGGTAGGCAG CGAACTCCTC ANGCTCCAGT TTCCGCATGA TCTTGCTTGG GAGCATGGTC
  721 TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT GATCAGGGCG
  781 ATATCCTCCT CNATGTCANG NCACTCGTCC CAGGANTCNA TCACGTNCAC GANACTCTCA
  841 GCATGGACNA TGGNCTTGAT CTTGNCTTGG NGCNCGTNNN ANTAGTGAAA NGNNCAGANN
  901 AGCCCCCCNN GNNNNNNNNC NNNNGNTGAT TTNCNTNNGN NNNNNNANCA GNNNNNNCCN
961 AGCNNNNANG NNNNNNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: Luc2 5'
Sequence file: RDB15800_A8Bqe.seq check
>D05196A7-4_A8Bq_PGKprp_F_C02_2018-03-19-14-24-28_08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCTCCNGG CCTTTCGNNC TGCAGCCCAA GCTTGGCAAT
   61 CCGGTACTGT TGGTANAGCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC
  121 CATTCTACCC ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT
  181 ACGCCCTGGT GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT
  241 ACGCCGAGTA CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA
  301 ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT
  361 TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG
  421 AGCTGCTGAA CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC
  481 TGCAAAAGAT CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG
  541 ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC
  601 CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG
  661 CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC
  721 GCACCGCTTG TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC
  781 CCGACACCGC TATCCTCAGC GTGGTGCCAT TTCACNACGG CTTCGGCATG TTCACCACGC
  841 NNGGCTACTT GATCTGCGGC TTTCGGGNCG TGCTCATGTA CCGCTTCNAG GANGANCTAT
  901 TCTTGCGCAG CTNGNNNGAN TATAANATTN NNTCTGNCNN NCNNGNNNCC ACNCTATTTN
961 NCNNNNTCNC NNANNNCNNN NNNATCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Deguchi, S., Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis. Oncogene 36 (32): 4629-4640 (2017). PMID 28368417. [link to RRC of NBRP]