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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-FcR gamma gRNA

Targeting construct of human Fc receptor gamma chain (FcR gamma).

Catalog number RDB15903
Resource name pX330-FcR gamma gRNA
Clone info. Expression vector of gRNA for targeting human Fc receptor gamma chain (FcR gamma) and human codon-optimized SpCas9.
Comment Effect of the targeting vecctor was confirmed by the depositor with immunoblotting.
Vector backbone pX330 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human FCER1G genomic DNA
Depositor|Developer Sada, Kiyonao |
Other clones in our bank

External Database
Human FCER1G

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chihara, K et al. Sci Rep. 7(1) : 11480, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15903 pX330-FcR gamma gRNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15903_A8Crp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3' element Reverse
          Sequence file: RDB15903_A8Cra.seq check
          >D05305A1-1_A8Cr_40pA3'elementRever_A01_01_ABI24.ab1
              1 GTTTGGGGGG GGGCGGGGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
             61 CCTTTTGCTG GCCTTTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
            121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
            181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
            241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
            301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGAGT AAGAGCAAGA CCACTGCGTT
            361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
            421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
            481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
            541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
            601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
            661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG
            721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC
            781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT
            841 TCACTCTCCC CATCTCCCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTTATTTTTT
          901 TAATTATTTT TGTGCAGCGA TGGGGGGCGG GGGGGGGGGG GGA
          //
          Primer: RDB13535#1
          Sequence file: RDB15903_A8Crb.seq check
          >D05305A1-1_A8Cr_RDB13535#1_B01_04_ABI24.ab1
              1 TGGGAATGCC TGAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC
             61 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
            121 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
            181 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
            241 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
            301 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
            361 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
            421 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
            481 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
            541 GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC
            601 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC
            661 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC
            721 CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG
            781 GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC
            841 GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG
            901 GGCCCTGACC CCCCAACTTC AAGAGCAACT TCGACCCTGT CGAGGATGCC CAAACTGCAG
            961 CTGAGCAGGA CACCTACGAC GAACGACTGC ACACCTGCTG GGCCAGATCG GCCGACAAGT
           1021 ACGGCGACCT GGCTTTCCTG ACGGACAGCA GCTGATCGAC GTCATCTTGC TTGAGCGACA
          1081 TTCCATG
          //
          Primer: BGH_rev
          Sequence file: RDB15903_A8Crc.seq check
          >D05305A1-1_A8Cr_BGH_rev2_C01_07_ABI24.ab1
              1 CACGGGCTAG GGAGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGAGC TCTAGGAATT CTTACTTTTT CTTTTTTGCC TGGCCGGCCT
            121 TTTTCGTGGC CGCCGGCCTT TTGTCGCCTC CCAGCTGAGA CAGGTCGATC CGTGTCTCGT
            181 ACAGGCCGGT GATGCTCTGG TGGATCAGGG TGGCGTCCAG CACCTCTTTG GTGCTGGTGT
            241 ACCTCTTCCG GTCGATGGTG GTGTCAAAGT ACTTGAAGGC GGCAGGGGCT CCCAGATTGG
            301 TCAGGGTAAA CAGGTGGATG ATATTCTCGG CCTGCTCTCT GATGGGCTTA TCCCGGTGCT
            361 TGTTGTAGGC GGACAGCACT TTGTCCAGAT TAGCGTCGGC CAGGATCACT CTCTTGGAGA
            421 ACTCGCTGAT CTGCTCGATG ATCTCGTCCA GGTAGTGCTT GTGCTGTTCC ACAAACAGCT
            481 GTTTCTGCTC ATTATCCTCG GGGGAGCCCT TCAGCTTCTC ATAGTGGCTG GCCAGGTACA
            541 GGAAGTTCAC ATATTTGGAG GGCAGGGCCA GTTCGTTTCC CTTCTGCAGT TCGCCGGCAG
            601 AGGCCAGCAT TCTCTTCCGG CCGTTTTCCA GCTCGAACAG GGAGTACTTA GGCAGCTTGA
            661 TGATCAGGTC CTTTTTCACT TCTTTGTAGC CCTTGGCTTC CAGAAAGTCG ATGGGATTCT
            721 TCTCGAAGCT GCTTCTTTCC ATGATGGTGA TCCCCAGCAG CTCTTTCACA CTCTTCAGTT
            781 TCTTGGACTT GCCCTTTTCC ACTTTGGCCA CCACCAGCAC AGAATAGGCC ACGGTGGGGC
            841 TGTCGAAGCC GCCGTACTTC TTAGGGTCCC AGTCCTTCTT TCTGGCGATC AGCTTATCGC
            901 TGTTTCCTCT TGGGCAAGGA TAGACTCTTT GCTGAAAGCC GCCCTGTCTG CACCTCGGGT
            961 CTTTTTCACG ATATTCACTT TGGGGCATGC TCAGCACTTT CCCGCCACGG TGGCAAAATC
           1021 CCGGCCCTTA TCCCACACGA TCTCCCCGGA TTTCGACGAT TTGTTCTCCG AATCAGAAGG
          1081 GCCGCCTTTC CGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Chihara, K., Syk-dependent tyrosine phosphorylation of 3BP2 is required for optimal FcRγ-mediated phagocytosis and chemokine expression in U937 cells. Sci. Rep. 7 (1): 11480 (2017). PMID 28904407.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl