C43(DE3)-based E. coli auxotroph host strain used for selective isotope labeling. Genotype: cysE;
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (T. Iwasaki et al., J. American Chem. Soc. 134, 19731-19738, 2012). Additional terms and conditions: Commercialization or third-party transfer of this strain is prohibited. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
Remarks | It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (T. Iwasaki et al., J. American Chem. Soc. 134, 19731-19738, 2012)。 付加的提供条件: 菌株の商品化や第三者移転は禁止されている。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | 実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。 組換え体提供にかかる書式が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15943 | YM138 | Bacteria in stab agar |
Materials & Methods section:
The YM138 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15943). |
Reference section:
Iwasaki, T., Fukazawa, R., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M.T., Gennis, R.B., Hasegawa, K., Kumasaka, T., Dikanov, S.A., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Bacteria host strains (en) (English text) |
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Featured content | Bacteria host strains (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15943_A8Eap1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Iwasaki, T., Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin. J. Am. Chem. Soc. 134 (48): 19731-19738 (2012). PMID 23145461. [link to RRC of NBRP] |
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reference | Lin, M.T., A rapid and robust method for selective isotope labeling of proteins. Methods 55 (4): 370-378 (2011). PMID 21925267. [link to RRC of NBRP] |
protocol | Lin, M.T., Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins. Methods Enzymol. 565: 45-66 (2015). PMID 26577727. [link to RRC of NBRP] |
2024.02.28
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