RDB15991 - AGP1UAS-CYC1pro-VTC1ORF

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AGP1UAS-CYC1pro-VTC1ORF (#RDB15991)

PCR template to create yeast strain of AGP1(UAS)-CYC1(pro)-VTC1(ORF).

Alternative name	AGP1(UAS)-CYC1(pro)-VTC1(ORF)
Clone info.	Plasmid clone as a PCR template to create yeast strain of AGP1_UAS-CYC1_pro-VTC1_ORF.
Comment	Expression was confirmed by the depositor with Northern blot. There are three substitutions in CYC1, TATA1:TATATATA to TACGCGTA, TATA3:TAAATATT to TAGGCCTT, TATA4:TTATACATT to TTGCGCATT.
Vector backbone	pBluescript II KS(+) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	S. cerevisiae VTC1 genomic DNA S. cerevisiae CYC1 genomic DNA S. cerevisiae AGP1 genomic DNA
Depositor\|Developer	Kokubo, Tetsuro \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (PLoS One, 12 (11), e0188435, 2017).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (PLoS One, 12 (11), e0188435, 2017).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (PLoS One, 12(11), e0188435, 2017)。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB15991	AGP1UAS-CYC1pro-VTC1ORF	Under QC test. Please contact us.	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The AGP1UAS-CYC1pro-VTC1ORF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15991).

Reference section:

Watanabe, K., Kokubo, T., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Watanabe, K., SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae. PLoS One 12 (11): e0188435 (2017). PMID 29176831. [link to RRC of NBRP]