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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSuperior.neo-U6snRNA (#RDB16712)

Expression vector of synthetic U6 small nuclear RNA (snRNA) oligonucleotide, H1 promoter.

Clone info. Expression vector of synthetic U6 small nuclear RNA (snRNA) oligonucleotide, H1 promoter.
Comment Expression was confirmed by the deposior with Urea-PAGE.
Vector backbone pSuperior.neo (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human U6 snRNA cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Yahara, M. et al. PLoS One, 12 (11): e0187813, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yahara, M. et al. PLoS One, 12 (11): e0187813, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16712 pSuperior.neo-U6snRNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSuperior.neo-U6snRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16712).

Reference section:

Yahara, M., Kitamura, A., Kinjo, M., U6 snRNA expression prevents toxicity in TDP-43-knockdown cells. PLoS One 12 (11): e0187813 (2017). PMID 29125873. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16712_A8Ilp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: PGK_N_rev ()
Sequence file: RDB16712_A8Ila.seq check
> CWKGGGAGSGGCTCCCTACCCGGTAGATTCGAACGCTGACGTCATCAACCCGCTCCAAGGAATCGCGGGCCCAGTGTCAC
    1 TAGGCGGGAA CACCCAGCGC GCGTGCGCCC TGGCAGGAAG ATGGCTGTGA GGGACAGGGG
   61 AGTGGCGCCC TGCAATATTT GCATGTCGCT ATGTGTTCTG GGAAATCACC ATAAACGTGA
  121 AATGTCTTTG GATTTGGGAA TCTTATAAGT TCCCTATCAG TGATAGAGAT CCCCGTGCTC
  181 GCTTCGGCAG CACATATACT AAAATTGGAA CGATACAGAG AAGATTTAGC ATGGCCCCTG
  241 CGCAAGGATG ACACGCAAAT TCGTGAAGCG TTCCATATTT TTAAGCTTAT CGATACCGTC
  301 GACCTCGAGG GGGGGCCCGG TACCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTTCGAGC
  361 TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA
  421 CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA
  481 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG
  541 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
  601 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
  661 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
  721 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
  781 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
  841 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
  901 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
  961 CGCTTTCTCA TAGCTCACGC TGTAGTATCT CAGTTCGGTG TAGTCGTTCG CTCAGCTGGC
 1021 TGTGTGCACG AACCCCCCGG TTCAGTGAAC GCTGCGCTAA TCGGTACTAT CGTCTTGGAG
1081 TCCAACCCGG TTAGGA
//
Primer: PGKpro_F ()
Sequence file: RDB16712_A8Ilb.seq check
> CKTCSSSGCGGTTCTCTCTTCTCATCTCCGGGCCTTTCGACCTGCAGCCCAAGCTAGCTTACCGGACTCAGATCAGCTTG
    1 ATTCTTCTGA CACAACAGTC TCGAACTTAA GGCTAGAGCC ACCATGATTG AACAAGATGG
   61 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA
  121 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT
  181 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG
  241 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA
  301 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA
  361 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT
  421 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC
  481 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC
  541 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT
  601 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT
  661 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG
  721 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT
  781 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC
  841 GGGACTCTGG GGTTCGAAAT GACCGACCAG CGACGCCCAA CCTGCCATCA CGATGGCCGC
  901 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGCG
  961 ATAGGATCGG GGATGCAGCY CCGGGGGATC ACTAGTTCTA GAGCGCGGCA CGCGTGGAGC
 1021 TCCATTCGCC TATAGTGRTT CGTATTACAT TCACTGCGGT CGATTTACAC GTYSKKAMCK
1081 TGGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yahara, M., U6 snRNA expression prevents toxicity in TDP-43-knockdown cells. PLoS One 12 (11): e0187813 (2017). PMID 29125873. [link to RRC of NBRP]