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pGL3-mouse RORgammat promoter (-710 to +68) (#RDB16746)

Luciferase reporter of the mouse retinoic acid-related orphan receptor gamma t (ROR gamma t) promoter (-710 to +68).

Clone info. Luciferase reporter of the mouse retinoic acid-related orphan receptor gamma t (ROR gamma t) promoter (-710 to +68).
Vector backbone pGL3 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rorc genomic DNA
Depositor|Developer Maruyama, Takashi |
 
Sequence (full) RDB16746zzt01.seq compiled by us

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16746 pGL3-mouse RORgammat promoter (-710 to +68) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-mouse RORgammat promoter (-710 to +68) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16746).

Reference section:

,

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16746_A8Ipp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: synthPA_F (Pr0257)
Region: Pause site,insert 5'
Sequence file: RDB16746_A8Ipa.seq check
>D05839D2_A8Ip_2_synthPA_F_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT ACGCTCTCNN TCNAAACNAA ACGAAACAAA ACAAACTAGC
   61 AAAATAGGCT GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCTATC GATAGGTACC
  121 GAGCTCTCAA CTTGTTGGTC CAGTGCAGTT GCTCTCTTCT GCTCTCCCTC CAACACCCCT
  181 CACCTCTCAA TTTGCTCTTC CTCCTCCACA CCTCCCCCCA CCACACCAGA AGTATGGGAA
  241 GCATCTAGAA GCCTGGAAAA TCCAGAGGTG GGCAGCATGG AGTTAGAGAG AGGCAAAGCA
  301 AGACATGTGC TGGGGGAACC CAAGCTGCAG GGAAGTATAT CTGATCCCAT CCTGGAGATC
  361 AGAGTGCACA CACACACACA CACACACACA CACAGAGGCT GTCTTTTCTC TTCACTCTTC
  421 CTGCCTCCCT TTCTTGCTAT TCTGTCACCT GTCCTAAAAA AGTTACAGAG ACCAGGTTCA
  481 AATCTCAACT CAATCACGAA TTGCCCTTGT GAATTCCTGG CAAGTCTTTT CTATTCTGGG
  541 CAGCAATTGG TTCATGATTA TGAATAATCA CTCGTGCCTG TAGGGGGTTA TCATGTGGGA
  601 TTCCAAAAAA CAAACAAAAA AATTCTGATT TACCTACAAG GCCTGGCAAA AACTCAGCAT
  661 ACAGTAGGTA ATTAATAAGT ACTAGCTCCC TTTCACCTGT TTTTCCTCAA GACTCCAGAC
  721 ACTGGTCCAC TGCTCTCTCC CTGANTCCCC TCCTGTGCCA GGACACTCTG CCAGCCACTC
  781 CTTTCCCCTG CCTGCTGACG GGCCANGTGC TCCCTCCCTC TTCCCTCCTC CCTCCNNTGN
  841 GCCCTGCTCC CNGCNNTCCT GGGGCAGCCA GGGCAGCAAG GACGGCACCN ANGGANCTAC
  901 CCNNTAGATC TGCNATCTAN NTAANCTNNN CNNNNCGGNA NNGNNGGNNN NNCCNCCNNG
961 GNANANGCCN AAAACNTAAA NNNNNCCCNN NNNNNNNN
//
Primer: Luc_R (Pr0146)
Region: Luc_R,insert 3'
Sequence file: RDB16746_A8Ipb.seq check
>16746_16746_B1EqF_2_Luc_R_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCT CTTNNNNNNC TTATGNNNGT TGCTCTCCNG
   61 CGGTTCCNTC TTNNNNNGGA TAGAATGNNN NNNNNGGCCT TTCTTTATGT TTTTGGCGTC
  121 TTCCATGGTG GCTTTACCAA CAGTACCGGA ATGCCAAGCT TACTTAGATC GCAGATCTAT
  181 GGGGTAGCTC CCTTGGTGCC GTCCTTGCTG CCCTGGCTGC CCAGGAGGGC AGGGAGCAGG
  241 GCCCAAGGGA GGGAGGAGGG AAGAGGGAGG GAGCACCTGG CCCGTCAGCA GGCAGGGGAA
  301 AGGAGTGGCT GGCAGAGTGT CCTGGCACAG GAGGGGACTC AGGGAGAGAG CAGTGGACCA
  361 GTGTCTGGAG TCTTGAGGAA AAACAGGTGA AAGGGAGCTA GTACTTATTA ATTACCTACT
  421 GTATGCTGAG TTTTTGCCAG GCCTTGTAGG TAAATCAGAA TTTTTTTGTT TGTTTTTTGG
  481 AATCCCACAT GATAACCCCC TACAGGCACG AGTGATTATT CATAATCATG AACCAATTGC
  541 TGCCCAGAAT AGAAAAGACT TGCCAGGAAT TCACNAGGGC AATTCGTGAT TGNNNNNNNA
  601 NTTGAACCTG GTCTCTGTAA CTTTTTTAGG ACANGTGACA GAATANCAAG AAAGGGANGC
  661 AGGAANAGTG NAGAGAAAAG ACAGCCTCTG NGTGTGTGTG TGTGTGNGTG TGTGNNNNNN
721 NNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,Luc 3'
Sequence file: RDB16746_A8Ipc.seq check
>16746_16746_B1EqF_2_SV40pA_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT TNNNNAATAN NNNNNTTTTT TNNCTGCATT CTAGTTGTGG
   61 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC
  121 TCTAGAATTA CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT
  181 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC
  241 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG
  301 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT
  361 CGGGAAGACC TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA
  421 ATTCAGCGGG AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA
  481 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT
  541 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAN
  601 CTCTCACACA CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGNA TCCAGATCCN
  661 CAACCTTCNN TTCNAAAAAN GNNNNNNCTT TANCGANCNN GCCCNGNNNN NNCNNNCCCN
721 NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original unpublished,