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Rat CYP51A1/pBSK- (#RDB16754)

Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1).

Clone info. Cloning vector of rat cytochrome P450 family 51 subfamily A member 1 (CYP51A1).
Vector backbone pBluescript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Rat CYP51A1 cDNA
Depositor|Developer Aoyama, Yuri |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Aoyama, Y. et al. J. Biochem. 119(5), 926-33, 1996) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Aoyama, Y. et al. J. Biochem. 119(5), 926-33, 1996）の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16754 Rat CYP51A1/pBSK- DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Rat CYP51A1/pBSK- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16754).

Reference section:

Aoyama, Y., Noshiro, M., Gotoh, O., Imaoka, S., Funae, Y., Kurosawa, N., Horiuchi, T., Yoshida, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16754_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: insert 5'
Sequence file: RDB16754_A8K1a.seq check
>D05837A2-A8K1_1_M13_-40_C01_03_ABI08.ab1
    1 CGGCCGGGTT AAATGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG
   61 AGGTCGACGG TATCGATAAG CTTGATATCG AATTCGGCGG AGGCCGGGGC CGCCTCCCGA
  121 CGCCCACCTA CTAGCTTCTC CAGCTCCATC TTTCCCGAGA AGCGGCAGGG ACCTCGGCCT
  181 CCAGCTTACC GGGCGGATCG AAGCAGCGGT CGGGATGGTA CTGCTGGGCT TGCTGCAGTC
  241 AGGCGGCTCG GTGCTCGGGC AGGCGATGGA GCAGGTGACA GGAGGCAACC TGCTTTCCAC
  301 GCTGCTCATC GCCTGCGCCT TCACGCTTAG CCTTGTCTAC CTGTTCCGCC TCGCAGTGGG
  361 CCACATGGTC CAGCTGCCCG CTGGAGCGAA AAGTCCGCCA TATATTTACT CTCCAATTCC
  421 GTTCCTTGGC CACGCGATAG CATTTGGGAA AAGTCCAATT GAGTTCCTGG AAAATGCATA
  481 TGAGAAGTAC GGACCTGTGT TTAGCTTTAC CATGGTGGGC AAGACTTTCA CTTACCTTTT
  541 GGGGAGTGAC GCAGCCGCCC TGCTCTTCAA CAGTAAGAAC GAAGACCTGA ATGCGGAAGA
  601 GGTCTACGGT CGGCTGACAA CGCCCGTGTT TGGGAAGGGA GTTGCATACG ACGTGCCAAA
  661 TGCAGTTTTC CTGGAACAGA AGAAAATACT AAAAAGCGGC CTTAACATAG CCCACTTCAA
  721 GCAGTATGTT TCTATCATTG AAAAAGAAGC GAAGGAGTAC TTTAAAAGTT GGGGAGAAAG
  781 CGGAGAAAGA AATGTGTTTG AAGCGCTGTC TGAGCTCATA ATTTTAACAG CTAGCCATTG
  841 CTTACATGGA AAGGAAATTA GAAGTCAGCT CAACGAGAAA GTGGCTCAGC TGTACGCAGA
  901 CCTGGGATGG AGGCTTTAGC CATGCCGCCT GGGCTGTTGC CAGGCTGGCT GCCCCTGCCC
  961 GAGTTTCAGG CGCAGGACAG GAGCCCAATC GGAGAGATCA AGAATATTTT TCTACAAGGC
 1021 CATCCAGAAC GGTAAGCTGT CAAAGAACTA GGTCGGAGGA CACTCGTCGA AACTCGACTG
1081 GAGTCCTACC GTACAAGGAA TTGGGGG
//
Primer: Reverse2 ()
Region: insert 3'
Sequence file: RDB16754_A8K1b.seq check
>D05837A2-A8K1_1-reverse2_A01_01_ABI08.ab1
    1 GTAAACAGGG ATTACGCCAA GCTCGAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC
   61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGGTT
  121 TTTTTTTTAA TTAAAATTAA AAATTCCACA AGGATTTGAT TTTGGATTTT TCTGGAACCA
  181 ATAACATAAG TACTCTTTAG TATTTATTAT TTTTAGGAAA TAAGGTTATC AAGATAAGGT
  241 CCAAGATTGC TGCCATCTCG CTGGAGTGGT GCTCTCAGTT AACACTACAA AGGGCACTCA
  301 CACCATCTAG AGCTTCGCAG AAAACTTATC CTTCACCCTC TCACATCTTT ACGCACAGAA
  361 GTGGCCCAAC CACACCAATG ACCCATTGCC TCTGTAGCAT CTGACCAGGC TTAAAACTTC
  421 CCAAACTAGT TCCAAGCCGG AATCTCCCTA CGGCAACGTG TGCTTCTGAA GTTAGTTACC
  481 TGGATGCTAT ATTCTGTATC ATCTCTGAAT ACCGAGGCTC CCCTAAACGA TTTCTAACAT
  541 AAACTTGGCT TCCCTGAAAT CCTATCAGAA CTATTATAAC TGTTTCAAAT GCCATTAGGT
  601 CTGACAAAAA TCACCGAGTT AATAAAACAT TAAAATCAAA CTGGCTTTCA GAACCACACA
  661 CTTAAAAAAC AGTACAGTAT GAAAGCTGTG TCAAAGCTTC ATTCTCTGCC AAGCTGCAGC
  721 TTGCAGTCCC GTCTCCACAC TGGCTCCTTG TTCCTTTCTT CATTTTGATC TTCGTTTGTA
  781 ACGGATTACT GGGTTTTCTG GGGTATGAAT CATTGTTGTA TAATTCACAC TGGGAAAATA
  841 TCCATTGATG AGGTCAAATT CATATAAACG AAGCATAGTG GACCAAATTG TCTTAATTTG
  901 AACATAGGCA AAATTTTCTC CAATACAACG ATGGCGCCCG GGCTCCAAAC GGGCACATAG
  961 GCAAACTTTC TCTCCCGACG CTGGGGTTGG TCCTGGTAGT AGCGGGTCAG ATTGAAAGTT
 1021 CCAAGCGTTC TACCAGAGTC TTTCAGCTCT TTGATGACAG TGGAGACACA CACCTTGAGT
1081 GTCCTGAGAA TGTGTACCTC AGCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Aoyama, Y., Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species. J. Biochem. 119 (5): 926-933 (1996). PMID 8797093. [link to RRC of NBRP]