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Oat CYP51G1m/pET17b (#RDB16758)

Expression vector of oat cytochrome P450 family 51 subfamily G member 1 (CYP51G1), modification of N-terminal region.

Clone info. Expression vector of oat cytochrome P450 family 51 subfamily G member 1 (CYP51G1), modification of N-terminal region.
Comment Deletion of ATGGATCTCC TCGCGGACGC AAAGCCCCAG TGGCTCCTCG CCGGCGCGGC GCTGCTGCTC GCCACGCTCG CCTTCCTCAA GGTCCTCTTC GGGTCCGGCG GCGGCGGCAA GCGT of nucleic acid sequence coding N-terminal 38 amino acid residues, and addition of ATGGCTAAGA AAACGAGTTC GAAAGGGAAG at this region.
Vector backbone pET-17b (plasmid)
Size of vector backbone 3.3 kb
Selectable markers Amp^r
Gene/insert name Avena sativa CYP51H10 cDNA
Depositor|Developer Aoyama, Yuri |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16758 Oat CYP51G1m/pET17b DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Oat CYP51G1m/pET17b was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16758).

Reference section:

, Master thesis.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16758_A8K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp ()
Region: insert 5'
Sequence file: RDB16758_A8K1a.seq check
>D05837A6_A8K1_1_T7_20bp_H02_23_ABI24.ab1
    1 GAACTTCCTC TAGAATATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGCTAAGAAA
   61 ACGAGTTCGA AAGGGAAGGC GCCGCCCACG ATCCCGGGGG CGCCCGTGGT GGGCGGCCTG
  121 CTGCGGTTCC TCAAGGGCCC GATCCCGCTC ATCCGGGCCG AGTACGCGCG CCTCGGCTCC
  181 GTCTTCACCG TCCCCATCCT CACGCGCCGG ATCACCTTCC TCATCGGGCC GGAGGTTTCG
  241 GGCCACTTCT TCAAGGGCAA CGAGTCCGAG ATGAGCCAGC AGGAGGTGTA CCGCTTCAAC
  301 GTGCCCACCT TCGGGCCCGG GGTCGTCTTC GACATCGACT ACCTGGTCAG GCAGGAGCAG
  361 TTCAGGTTCT TCACCGAGGC GCTCAAGTCT AACAAGCTCC GCAGCTACGT CGACATGATG
  421 GTTGCTGAGG CCGAGGACTA CTTCTCGACG TGGGGAGAAA GCGGCACTGT GGATTTGAAG
  481 TATGAGTTGG AGCACCTCAT CATACTGACT GCTAGCCGAT GTTTGTTGGG AAGGGAGGTG
  541 CGAGAAAAGT TATTTGATGA TGTCTCTGCT CTATTCCATG ATCTCGACAA TGGGATGCAG
  601 CCAATCAGTG TCATCTTCCC CTACCTCCCG ATCCCTGCGC ACCGCAAACG TGACCAGGCA
  661 CGGGCACGTT TGGCGGAAAT CTTCGCCACT ATCATCAAAT CTCGCAAGGC TTCTGGACAG
  721 TCTGAGGAAG ACATGCTGCA GTGCTTCATT GATTCCAAGT ACAGGAACGG CCGCCCCACC
  781 ACAGAAATTG AGGTGACTGG GCTCCTCATT GCAGCGCTAT TTGCTGGGCA GCATACTAGC
  841 TCTATCACCT CAACCTGGAC CGGGGCGTAC CTTCTTAAGT TCCAGCAGTA CTTTGCGGAG
  901 GCTGTAGAGG AGCAGAAGAG GGTCATGAAG AGGCACGGTG ACAAGATCGA TCATGACATC
  961 TTGGCGGAGA TGGATGTCCT CTACCGGTGC ATCAAGGAGG CACTCCCGTC TCCACCCGCC
 1021 ACTCATCATG TTTGCTTCGC CAGTCACATA CTGACTTCAG CGTGACCACG AGAAGAGGCA
 1081 AAGAGTATGA TATCCCCAAA GGCCCATATC GGTCGCACGT CTCGTTCTTC GCTCACAGGG
1141 CTCCCTCATA TCTTCCAGGA ACCCGGGAAC TCTCGAAT
//
Primer: T7 terminator ()
Region: insert 3'
Sequence file: RDB16758_A8K1b.seq check
>D05837A6_A8K1_1_T7 Terminator_A03_03_ABI24.ab1
    1 GCGTAGCTTC TTTCGGGCTT TGTTAGCAGC CGGATCTGCT CGAGCGGCCG CCAGTGTGAT
   61 GGATATCTGC AGAATTCCTA ATGGTGGTGA TGATGGTGGT TATCGACAAC GAGCTTCCGC
  121 CGCTTGTAGT TGACCATCAC CTCACCCTTG ATGCCCACGA CCATCGCGTT CCAGTCGTTC
  181 TCGGGGAACG GCGAGACAAG CTCGAACTCA AAGTTCCTCA GCAGGTGCGT CCATATCGCC
  241 TTGATCTGCA GGTACGCGAA GGGCTCACCG AGGCACCCGT GCCTGCCACC CCCGAATGAG
  301 ATGTACGAGA AGGCACCAGC AGCCTTGTCT TCCTCCCTCC CAGGTGCAAA CCGGTCAGGG
  361 TCATACGAGT CAGGGTTCTT GAAGATATGA GGGAGCCTGT TGGCGAAAGA AGGAGACGTT
  421 GCGACGATAT GGCCCTTGGG GATATCATAC TCTTTGCCTT CTCTCGTGGT CACGCTGAAG
  481 TCAGTATGTG ACTGGCGAAG CAACATGATG AGTGGCGGGT GGAGACGGAG TGCCTCCTTG
  541 ATGCACCGGT AGAGGACATC CATCTCCGCC AAGATGTCAT GATCGATCTT GTCACCGTGC
  601 CTCTTCATGA CCCTCTTCTG CTCCTCTACA GCCTCCGCAA AGTACTGCTG GAACTTAAGA
  661 AGGTACGCCC CGGTCCAGGT TGAGGTGATA GAGCTAGTAT GCTGCCCAGC AAATAGCGCT
  721 GCAATGAGGA GCCCAGTCAC CTCAATTTCT GTGGTGGGGC GGCCGTTCCT GTACTTGGAA
  781 TCAATGAAGC ACTGCAGCAT GTCTTCCTCA GACTGTCCAG AAGCCTTGCG AGATTTGATG
  841 ATAGTGGCGA AGATTTCCGC CAAACGTGCC CGTGCCTGGT CACGTTTGCG GTGCGCAGGG
  901 ATCGGGAGGT AGGGGAAGAT GACACTGATT GGCTGCATCC CATTGTCGAG ATCATGGAAA
  961 TAGAGCAGAG ACATCATCAA ATAACTTTTC TCGCACCTCC CTTTCCCAAC AAACATCGGC
 1021 TAGCAGTCAG TATGATGAGG TGCTCCAACT CATACTTTCA AATCCACAGT GCCGCTTTTC
 1081 TCCCCCACGT CGAGAAGTAG TCATCGGGCC TCAGCATCAA TCATGTCGAA CGTTAGCCTG
 1141 CGGAGCCTGG TAGACGTGAG CGCCCTCGGG TGAAAGAACC TTGAACCGGC TCTCGACCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original , Master thesis.