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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Oat CYP51H10/pBKS-

Cloning vector of oat cytochrome P450 family 51 subfamily H member 10 (CYP51H10), tagged with His at C-terminus.

Catalog number RDB16759
Resource name Oat CYP51H10/pBKS-
Clone info. Cloning vector of oat cytochrome P450 family 51 subfamily H member 10 (CYP51H10), tagged with His at C-terminus.
Vector backbone pBluescript KS- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Avena sativa CYP51G1 cDNA
Depositor|Developer Aoyama, Yuri |
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External Database
Avena sativa CYP51G1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kunii, M et al. Biol. Pharm. Bull. 35(5), 801-04, 2012) is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kunii, M et al. Biol. Pharm. Bull. 35(5), 801-04, 2012)の引用を必要とする。
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          RDB16759 Oat CYP51H10/pBKS- DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Oat CYP51H10/pBKS- was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16759).

          Reference section:

          Kunii, M., Kitahama, Y., Fukushima, E.O., Seki, H., Muranaka, T., Yoshida, Y., Aoyama, Y., beta-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. Biol. Pharm. Bull. 35 (5): 801-804 (2012). PMID 22687421. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16759_A8K1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 ()
          Region: insert 5'
          Sequence file: RDB16759_A8K1a.seq check
          >D05837A7_A8K1_2_M13_-40_B03_06_ABI24.ab1
              1 AAGCCAGCGA GCGCGCGTAA TACGACTCAC TATAGGGCGA ATTGGAGCTC CACCGCGGTG
             61 GCGGCCGCTC TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCGATCCG TCGACATGGA
            121 CATGACAATT TGCGTCGTTT GGTTGGTCTT AGCAATTATA TCCATCGCTG CAGTAGTATC
            181 CAAGAGTTCA AAGCGAAGCA ATGCCTCTGA TTCAGTGGTG ACACGACCAC CTCCACCGGT
            241 GGTGACAGGA ATTGATCTCC TCAAGTTCTT ACATGCTCTT TGTAGAAAGG ACCCTGAAGC
            301 TGCAATGATG TATCTGTATA ACAAGTTAGG CAGTATTTTC ACATTAAGTT TTTTGTGGAA
            361 AAGAGTAACC ATCTTGATTG GGCACGAGGC CTCCATTCCT TTCTTTCATG GTTTGGAGTC
            421 AGATGTTTCA CAAGGAAATT TCAATGAGTT CACCGTGCCA ATGTTCGGCA AAGAGAATGG
            481 GTATGCTGTG GAATATGCTA CTCGAATTGA GCAGTCTCGC TTCTTCTATG ATTCTCTAAA
            541 GGCATCGCAG CTGAGGAGCC ATGTTGATCT CATTCGACAG GAAGTGGAGG AGTACTTTGC
            601 AAAATGGGGA GACGAGGGTG AAGTCGATCT GAAACAAGAG TTCACCAAGT TACTCATGTT
            661 GATTGCTGGT CGCTGCCTAC TTGGAAGTGA GGTCCGAGAT ACGATATTTG GTGAGTTCTA
            721 CACATTGTTC GCTGATATTG AGGAGGGGGT CAACTTGTTC AGTTACATGT TCCCATATAT
            781 GCCGGTTCCA GTAAACAACC GACGAGACAG AGCACAAATG AAGCTTACAA GTATAGTGTC
            841 TGAGATTGTG AGGTCAAGAA AGAGATGCAA CCGCGTCGAG GATGATATGC TGCAGAGACT
            901 GATAGATTCC AGATATAAAG ATGGTCGTCC AACAACTGAA GGGGAGGTTT CCGGGATGAT
            961 CATTGGACTT ATATTTGCTG GAAAGCACAC AAGTACAATC ACTGCCTCCT GGACCGGAGC
           1021 TTGCCTTTTG ACCCATCCAA AATTCCTAGT GCTGCTGTCG AGGAGCAAAG CAATGATGAG
           1081 TAAATACAAG GATATATAGA CTACATATCC TGTCAGAAAA TGGAAGATTT GGCATAGGTG
          1141 CCATCCAAGA AGGCAGTCGG ATGTAATCCC GCCAGCGGGC CCGGGGTGTT TGGC
          //
          Primer: Reverse2 ()
          Region: insert 3'
          Sequence file: RDB16759_A8K1b.seq check
          >D05837A7_A8K1_2_reverse2_C03_09_ABI24.ab1
              1 AAGGAAAATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGGT
             61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATCCGCTAGC CTAATGGTGG
            121 TGATGATGGT GGTTTGCAGG CATACGACAT CTCTTGTAAC TTACCATTAC TTTGCCTTTA
            181 GGCTCTATTA TAAACTTGCT CCAATCTTGC TTGGGAAATG GAGAAGTCAG TTTAAGATCA
            241 AAATTTCTCA GCAAATGGCT CCAGATAATC TTTATTTGCA TGAAAGCAAA GGACTCGCCG
            301 GCGCAAGCAT GCCTTCCAGC ACCAAAAATT GTGTAAGAGA GTTTACCCCC AATTTTATCC
            361 TCCTCTCTTC CAACACGAAA CCGATCCGGA TCATAGACCT CGGGGTCCTT GTAAGTGTGT
            421 GGCACCTTAC CTGTTAGCAT TACAAGATGT GCTAACGTGG TACCTTTAGG GATACCATAT
            481 TCACCTCCCT CTCTTGTCTG CACACTGATC TCCTTCAGTG TCTTGCGCAG CAACACCGGC
            541 GCTGCGGGAT ACATCCGACC TGCCTCTTTG ATGCAACTAT GCAAAATCTC CATTTCTGAC
            601 AGGATATTGT AGTCTATATT ATCCTTGTAT TTACTCATCA TTTGCTTTTG CTCCTCGACA
            661 GCAGCACCTA GGAATTTTGG ATGGGTCAAA AGGCAAGCTC CGGTCCAGGA GGCAGTGATT
            721 GTACTTGTGT GCTTTCCAGC AAATATAAGT CCAATGATCA TCCCGGAAAC CTCCCCTTCA
            781 GTTGTTGGAC GACCATCTTT ATATCTGGAA TCTATCAGTC TCTGCAGCAT ATCATCCTCG
            841 ACGCGGTTGC ATCTCTTTCT TGACCTCACA ATCTCAGACA CTATACTTGT AAGCTTCATT
            901 TGTGCTCTGT CTCGTCGGTT GTTTACTGGA ACCGGCATAT ATGGGAACAT GTAACTGAAC
            961 AAGTTGACCC CCTCCTCAAT ATCAGCGAAC AATGTGTAGA ACTCACCAAA TATCGTATCT
           1021 CGGACCTCAC TTTCCAAGTA GGCAGCGACC AGCAATCAAC ATGAGTAACT TGGTGAACTC
           1081 TGTTCAGATC GACTTCACCT CGTCTCCCAT TTGCAAGGTA CTCTCACACT GTCGGAATGG
          1141 AATCACATGC TTCTCATCTG CACTGCTATG GATCATAGGA GGAAGCGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kunii, M., beta-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors. Biol. Pharm. Bull. 35 (5): 801-804 (2012). PMID 22687421. [link to RRC of NBRP]

          2023.08.17

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