RDB16773 - pCMV-ChRFR-Venus

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMV-ChRFR-Venus (#RDB16773)

Mammalian expression vector encoding a mammalian codon-optimized chopFR.

Clone info.	Mammalian expression vector encoding a mammalian codon-optimized chopFR (channelopsin/fast-receiver) tagged with Venus at the C-terminus under CMV promoter.
Comment	Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone	pVenus-N1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions	LB+Kan, 37oC
Gene/insert name	Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Depositor\|Developer	Ishizuka, Toru \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wang, H. et al., J. Biol. Chem. 284 (9): 5685, 2009)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16773	pCMV-ChRFR-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-ChRFR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16773).

Reference section:

Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16773_A8L5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward () Sequence file: RDB16773_A8L5a.seq
>D06115A5_A8L5_3_CMV_Forward_E03_15_ABI24.ab1 1 CGTCAACTCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT 121 CAAGCTTCGA ATTCCACCAT GGCGAGAAGA CCCTGGCTGC TGGCTCTGGC TCTGGCCGTC 181 GCCCTGGCTG CCGGTTCCGC TGGTGCCTCA ACTGGCTCCG ATGCTACTGT CCCCGTGGCC 241 ACACAGGACG GCCCTGATTA CGTCTTCCAC CGAGCTCATG AGAGGATGCT GTTTCAGACT 301 TCCTATACCC TGGAAAACAA TGGGTCTGTG ATCTGCATTC CCAACAATGG CCAGTGCTTC 361 TGTCTGGCAT GGCTCAAGTC TAACGGAACT AATGCCGAGA AACTGGCCGC TAACATCCTC 421 CAGTGGATTA CCTTCGCCCT GAGCGCCCTG TGCCTCATGT TTTACGGCTA TCAGACATGG 481 AAGTCCACTT GTGGATGGGA GGAAATCTAC GTGGCCACTA TCGAGATGAT CAAGTTCATC 541 ATTGAATACT TCCACGAGTT TGACGAACCT GCAGTGATCT ATAGCTCCAA CGGCAATAAG 601 ACAGTCTGGC TGAGATACGC TGAGTGGCTG CTCACTTGTC CAGTGATCCT GATTCATCTC 661 AGCAACCTGA CAGGGCTCTC TAATGACTAT AGTAGGAGAA CTATGGGCCT GCTCGTGAGT 721 GATATCGGGA CCATTGTCTG GGGTGCCACT TCAGCTATGG CAACCGGATA CGTGAAAGTC 781 ATCTTCTTTT GCCTGGGGCT CTGTTATGGT GCTAACACAT TCTTTCACGC AGCCAAGGCA 841 TACATCGAGG GCTATCATAC CGTGCCTAAA GGACGGTGCC GCCAGGTGGT CACAGGGATG 901 GCTTGGCTGT TCTTTGTCTC TTGGGGGGAT GTTCCCAATC CTGTTTATTC TCGGTCCCGA 961 AAGGGTTCGG TGTGCTGTCA GTCTACGGGC AGCACCGTGG GACACACAAA TCAATTGACC 1021 TGATGAGTAA GAACTGTTTG GGGCCCTGCT CGGACACTAT CTGAGAGTGC TCAATCAACG 1081 GAAGCCATAT CATGAATTCC ATGGAGAAAA TATATTTTCG GGAAGGAAAC TCA //
Primer: _Venus-N_R () Sequence file: RDB16773_A8L5b.seq
>D06115A5_A8L5_3_Venus-N_R_F03_18_ABI24.ab1 1 TCGGTCGGAG CTGACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC 61 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTGG ATCCTTTCCA 121 GTGCCTTTGT TGACTGCTCC AGCCTCAGCC TCATCCTCCA CGAGTGTTTC CACCTCAATT 181 TCGGTTCCGC CGATATTCAG TTTTGTGGTC TTCCGAATAT CTCCATGAAT CAGGATATGC 241 TCGTGGATGA GCACTCTCAG ATAGTGTCCG AGCAGGCCCC AACAGTTCTT ACTCATCAGG 301 TCAATGATTG TGTGTCCCAC GGTGCTGCCG TAGACTGACA GCACACCGAA CCCTTCGGGA 361 CCGAGAATAA ACAGGATTGG GAACATCCCC CAAGAGACAA AGAACAGCCA AGCCATCCCT 421 GTGACCACCT GGCGGCACCG TCCTTTAGGC ACGGTATGAT AGCCCTCGAT GTATGCCTTG 481 GCTGCGTGAA AGAATGTGTT AGCACCATAA CAGAGCCCCA GGCAAAAGAA GATGACTTTC 541 ACGTATCCGG TTGCCATAGC TGAAGTGGCA CCCCAGACAA TGGTCCCGAT ATCACTCACG 601 AGCAGGCCCA TAGTTCTCCT ACTATAGTCA TTAGAGAGCC CTGTCAGGTT GCTGAGATGA 661 ATCAGGATCA CTGGACAAGT GAGCAGCCAC TCAGCGTATC TCAGCCAGAC TGTCTTATTG 721 CCGTTGGAGC TATAGATCAC TGCAGGTTCG TCAAACTCGT GGAAGTATTC AATGATGAAC 781 TTGATCATCT CGATAGTGGC CACGTAGATT TCCTCCCATC CACAAGTGGA CTTCCATGTC 841 TGATAGCCGT AAAACATGAG GCACAGGGCG CTCAGGGCGA AGGTAATCCA CTGGAGGATG 901 TTAGCGGCCA GTTTCTCGGC ATTAGTTCCG TTAGACTTGA GCCATGCCAG ACAGAAGCAC 961 TGGCCCATTG TTTGGGAATG CAGATCACAG ACCATTGTTT TTCCCAGGGT ATAGGAAAGT 1021 CTGAAACCAG CCATCTCCTC ATGAGCTTCG ATGAGACGTT ATTCAGGTCC GTCCTGTGTG 1081 CACAGGACAG GTAGCAATCG GAGTCCAAGT //
Primer: SV40polyA-R () Sequence file: RDB16773_A8L5c.seq
>D06115A5_A8L5_3_SV40polyA-R_G03_21_ABI24.ab1 1 AAAAATGGCA GTTTTAAGCA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA 61 GAGTCGCGGC CGCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA 121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT 181 GGTAGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT 241 GCTGGTAGTG GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT 301 TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT 361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT 421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC 481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA 541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA 601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA 661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC 721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC 781 CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCTTTCCAGT GCCTTTGTTG ACTGCTCCAG 841 CCTCAGCCTC ATCCTCCACG AGTGTTTCCA CCTCAATTTC GGTTCCGCCG ATATTCAGTT 901 TTGTGGTCTT CCGAATATCT CCATGAATCA CGATATGCTC GTGGATGAGC ACTCTCAGAT 961 AGTGTCCGAG CAGGCCCCAA CAGTTCTTAC TCATCAGGTC AATGATTGTG TGTCCTCACG 1021 GGTGCTGCTC GTAGACTGAC AGCACCACCC GAACTCCTTC GGGGAACCGA AGAATAAACA 1081 GGATTTGGGC AACCATTCCC CTCGAGAGAA CAAAGGATCA GCCTAAGCTC ATCCCCTGGT 1141 GAAACTAAT //
Primer: SV40pro_F Sequence file: RDB16773_A8L5a.seqd.seq
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Wang, H., Molecular determinants differentiating photocurrent properties of two channelrhodopsins from chlamydomonas. J. Biol. Chem. 284 (9): 5685-5696 (2009). PMID 19103605. [link to RRC of NBRP]