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pEGFP-C1-Rab38-T23N (#RDB16899)

Expression vector of mouse Rab38 constitutive negative mutant, T23N, fused with EGFP at N-terminus.

Clone info. Expression vector of mouse Rab38 constitutive negative mutant, T23N, fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab38 cDNA
Reference of insert sequence AB232634 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB16899zzt01.seq compiled by us

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol. Cell 20, 2900-2908, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16899 pEGFP-C1-Rab38-T23N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab38-T23N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16899).

Reference section:

Ishida, M., Ohbayashi, N., Maruta, Y., Ebata, Y., Fukuda, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16899_A9A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',insert (T23N)
Sequence file: RDB16899_A9A8a.seq check
>D06060I1_A9A8_1_EGFP-C2_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN CNCNTGGTCC TGCTGGNNTT CGTGNNCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGCAGACA CCTCACAAGG
  121 AGCACCTGTA CAAGCTGCTG GTGATCGGCG ACCTGGGTGT GGGCAAGAAC AGCATTATCA
  181 AGCGCTATGT GCACCAAAAC TTCTCCTCGC ACTACCGGGC CACCATTGGT GTGGACTTCG
  241 CGCTGAAGGT GCTCCACTGG GACCCAGAGA CGGTGGTGCG CTTGCAGCTC TGGGACATTG
  301 CTGGTCAAGA AAGATTTGGA AACATGACAA GAGTTTATTA CCGGGAAGCT ATGGGGGCAT
  361 TTATTGTTTT TGATGTCACC AGACCAGCCA CATTTGAAGC CGTGGCAAAG TGGAAAAATG
  421 ATTTGGACTC AAAGTTAACG CTCCCTAATG GTAAGCCAGT GTCAGTGGTT CTGTTGGCCA
  481 ACAAATGTGA CCAAGGGAAG GATGTGCTTA TGAACAATGG ACTCAAGATG GACCAGTTCT
  541 GCAAGGAGCA TGGCTTCGTA GGATGGTTTG AAACATCAGC CAAGGAAAAC ATAAACATTG
  601 ATGAAGCCTC AAGATGCCTG GTCAAGCACA TACTTGCAAA TGAGTGTGAC CTCCTAGAGT
  661 CTATAGAACC GGACATTGTG AAGCCCCATC TCACATCGCC CAAGGTTGTC AGCTGCTCTG
  721 GCTGTGCCAA ATCCTAGAAT CACTAGTGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG
  781 ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT
  841 TGCTTTAAAA AACCTCCCAC ACCTCCCCCN GANNNTGAAA CATAAAATGA ATGNAATTGN
  901 TGTTGNNNAC TNGTTTATTG CAGCTTATAA TGGNTNNNAA TAAAGCAATA NCATCNCAAA
961 NTTTCNNNAA TAAAGCANTT TTTTTCNNTG NNNNCNANNN NNNNNTNNNN NNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert (T23N)
Sequence file: RDB16899_A9A8b.seq check
>D06060I1_A9A8_1_SV40polyA_R2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNANNNNNN NTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA
   61 GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA
  121 TTATGATCAG TTATCTAGAT CCGGTGGATC CCGGGCCCGC GGTACCGTCG ACTGCAGAAT
  181 TCACTAGTGA TTCTAGGATT TGGCACAGCC AGAGCAGCTG ACAACCTTGG GCGATGTGAG
  241 ATGGGGCTTC ACAATGTCCG GTTCTATAGA CTCTAGGAGG TCACACTCAT TTGCAAGTAT
  301 GTGCTTGACC AGGCATCTTG AGGCTTCATC AATGTTTATG TTTTCCTTGG CTGATGTTTC
  361 AAACCATCCT ACGAAGCCAT GCTCCTTGCA GAACTGGTCC ATCTTGAGTC CATTGTTCAT
  421 AAGCACATCC TTCCCTTGGT CACATTTGTT GGCCAACAGA ACCACTGACA CTGGCTTACC
  481 ATTAGGGAGC GTTAACTTTG AGTCCAAATC ATTTTTCCAC TTTGCCACGG CTTCAAATGT
  541 GGCTGGTCTG GTGACATCAA AAACAATAAA TGCCCCCATA GCTTCCCGGT AATAAACTCT
  601 TGTCATGTTT CCAAATCTTT CTTGACCAGC AATGTCCCAG AGCTGCAAGC GCACCACCGT
  661 CTCTGGGTCC CAGTGGAGCA CCTTCAGCGC GAAGTCCACA CCAATGGTGG CCCGGTAGTG
  721 CGAGGAGAAG TTTTGGTGCA CATAGCGCTT GATAATGCTG TTCTTGCCCA CACCCAGGTC
  781 GCCGATCACC AGCAGCTTGT ACAGGTGCTC CTTGTGANGT GTCTGCATGG ATCTGANTCC
  841 GGANTTGTAC AGCTCGTCCA TGCCGANAGT GATNCNGGCG GCGGTCACGA ACTNCANCAN
  901 GANCNTGTGA TCGCNCTTCN CNNTNGGGNC TTNNCTCNNN NCGGANNGGG NGNTCNNNNN
961 NGGNNGNCGG GNANCANCNC GGGGNCNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB16899_A9A8c.seq check
>D06060I1_A9A8_1_CMV_Forward_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 ACCCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAN
  781 TTCGTGANCG CCGCCGGGAT CACTCTCGGC ATGGNCGANC TGTACNAGTC CGGANTCAGA
  841 TCCNTGCAGA CACCTCACAA GGANCACCTG TACAAGCTGC TGGNGNTCGG CGANNNNNNT
  901 GNGGGCNAGA ANNGCATTAT CNANCGCTNT GNGCNNNNAA NNNTCNNNNN GCACTACCNG
961 GNCNNCNNNN NNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB16899_A9A8d.seq check
>D06060I1_A9A8_1_SV40pro_F_V2_E06_15_ABI24.ab1
    1 NNNNNNNNNN NGNNCNNNGC TTTTGCAAGN NCGATCAAGA GNNAGGNTGA GGNTCGTTTC
   61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
  601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG
  721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
  781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
  841 TTGACGANTT CTTCTGANCG GGANTCTNGG GTTCGAAATG ANCGACCAAG CGACGCCCNA
  901 CCTGCCNTCA CGAGATTTCG ANNNCNCCGC CNNCTTCTAT GAAAGGNTGG NNTTCNNNNN
961 NNTTTTNNNG GNNNNCNNNN NGNNNGATNN NNCNNNNNNN GGNNNNNNNN NN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert (T23N)
Sequence file: RDB16899_A9A8e.seq check
>16899_16899_B1JdF_mVenus-M_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANNGTN NNNCTTCNNN NATCCGCCNC NNCATCNNNG
   61 ACNGNNNCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
  121 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG
  181 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
  241 TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGCAGAC ACCTCACAAG GAGCACCTGT
  301 ACAAGCTGCT GGTGATCGGC GACCTGGGTG TGGGCAAGAA CAGCATTATC AAGCGCTATG
  361 TGCACCAAAA CTTCTCCTCG CACTACCGGG CCACCATTGG TGTGGACTTC GCGCTGAAGG
  421 TGCTCCACTG GGACCCAGAG ACGGTGGTGC GCTTGCAGCT CTGGGACATT GCTGGTCAAG
  481 AAAGATTTGG AAACATGACA AGAGTTTATT ACCGGGAAGC TATGGGGGCA TTTATTGTTT
  541 TTGATGTCAC CAGACCAGCC ACATTTGAAG CCGTGGCAAA GTGGAAAAAT GATTTGGACT
  601 CNAAGTTAAC GCTCCCTAAT GGTAAGCCNG TGTCAGTGGT TCNGTTGNNC NACNAATGNG
  661 ANCNNNGNNA NGNANGTGCT TNNGANCNNT GGNNTCANNN GGACNNNTTC TGNNNGNNNC
721 NNNNNNNCNN NNNNNNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes. J. Cell Sci., 125 (Pt 21): 5177-5187 (2012). PMID 22854043. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]