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human TICAM-1 expression vector (#RDB16923)

Expression vector of human TICAM-1.

Clone info. Expression vector of human Toll-interleukin 1 receptor domain (TIR)-containing adaptor molecule 1 (TICAM-1).
Comment The full-length human TICAM-1 was cloned from mRNA of the human fibroblast cell line MRC-5. Expession was confirmed by the depositor.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TICAM1 cDNA
Depositor|Developer Seya, Tsukasa |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) and an acknowledgment to the DEPOSITOR are requested. Basic research use only. Do not pass the plasmids to the third persons without permission.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Oshiumi, H. et al. Nat. Immunol. 4(2) : 161-167, 2003) を引用し、謝辞の表明を必要とする。基礎研究に限る。許可なく第3者に渡すことを禁じる。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16923 human TICAM-1 expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TICAM-1 expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16923).

Reference section:

Oshiumi, H., Matsumoto, M., Funami, K., Akazawa, T., Seya, T., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16923_A9B7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Region: EF-1a pro, insert 5'
Sequence file: RDB16923_A9B7a.seq check
>D06030A1_A9A7_3_EF1a-C_F_B01_04_ABI24.ab1
    1 CATGGGGATT TGCCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG
   61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC
  121 CTCGAGTCAC CACCTCCAGC TTCTGCTGCC GGAGGCTGCA CCCACCTGTG CCCATGGCCT
  181 GCACGGGCCC ATCACTTCCT AGCGCCTTCG ACATTCTAGG TGCAGCAGGC CAGGACAAGC
  241 TCTTGTATCT GAAGCACAAA CTGAAGACCC CACGCCCAGG CTGCCAGGGG CAGGACCTCC
  301 TGCATGCCAT GGTTCTCCTG AAGCTGGGCC AGGAAACTGA GGCCAGGATC TCTCTAGAGG
  361 CATTGAAGGC CGATGCGGTG GCCCGGCTGG TGGCCCGCCA GTGGGCTGGC GTGGACAGCA
  421 CCGAGGACCC AGAGGAGCCC CCAGATGTGT CCTGGGCTGT GGCCCGCTTG TACCACCTGC
  481 TGGCTGAGGA GAAGCTGTGC CCCGCCTCGC TGCGGGACGT GGCCTACCAG GAAGCCGTCC
  541 GCACCCTCAG CTCCAGGGAC GACCACCGGC TGGGGGAACT TCAGGATGAG GCCCGAAACC
  601 GGTGTGGGTG GGACATTGCT GGGGATCCAG GGAGCATCCG GACGCTCCAG TCCAATCTGG
  661 GCTGCCTCCC ACCATCCTCG GCTTTGCCCT CTGGGACCAG GAGCCTCCCA CGCCCCATTG
  721 ACGGTGTTTC GGACTGGAGC CAAGGGTGCT CCCTGCGATC CACTGGCAGC CCTGCCTCCC
  781 TGGCCAGCAA CTTGGAAATC AGCCAGTCCC CTACCATGCC CTTCCTCAGC CTGCACCGCA
  841 GCCCACATGG GCCCAGCAAG CTCTGTGACG ACCCCCCAGG CCAGCTTGGT GCCCGAGCCT
  901 GTCCCCGGTG GCTGCCAGGA GCCTGAGGAG ATGAGCTGGC CCGCCATCGG GGGAGATTGC
  961 TCAGCCCACC AGAGCTGCCA AGCAGCCTCA CCTTCCTGGC TTTCCCGAGT GGGCCCCAGA
 1021 ATGCCACTCC ACTGGCCTCC TGATACCTCC GGCAGCTTCC AGAACTAGCA TCAACTTACC
1081 TAGTTGGAAG TGGCACTCGA GG
//
Primer: hGCSF_R ()
Region: hGCSF 3'LTR + poly(A), insert 3'
Sequence file: RDB16923_A9B7b.seq check
>D06030A1_A9A7_3_hGCSF_R_C01_07_ABI24.ab1
    1 GTGGAACTGC ATCTCTCAAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGT CGCGGCCGCG
   61 GGAATTCGAT TCGGGATCCC ACCACCAAGA CCCTTCACCC AGAAATAGAG AGATTGGAAT
  121 TGTAAACACC GTATCCAGTT CTGACCACCC TGAAAGCCAG GGCATCATCG CGCCCGTTTT
  181 GTCCTAAATG AAGGGCTCCC GGACAATGTC CTGAAAGTGG CAGATGACCT CATCTTCCAC
  241 TGTCCACCGG GCACAGGGCA GACCGGGGTG CTCTATGGGG TCCTGGCCGA TGCCTGGGTC
  301 CAGGGGTGTT CCCCAGGTGG TCAGGCAAGG ACACGCGGTC ATTCTGCCTC CTGCGTCTTG
  361 TCCTCGGGCG CCTGGGACCC TCTCTGGTTC CACATGTGGT TGTTCAGCCC CAGCTGTACC
  421 ATCTGTGCGT GGTGGATAAT GAGGGGTTGC AGCCCTGGGC TCTGAGGGGG TGCGGGTGAG
  481 GCCGTAGGGA AGGCTGGGGA CTGCGGGAAG GGCAGTGACT GTGGAAAGGC TGCTGGCTGT
  541 GGGGAGGGCG GTGGGGGGGT GCCAGCCTGC CATGCGTGCA GGGGTGGCGG CTGCGGGCAC
  601 CCCGGCCAAG TGGGAAAGGG TGGCGGGGCT CCCAGGGGCA CCTGGCCCCC AAAGGGCATT
  661 CGAGCCCCAT AGGGCGCCCC AGTCCCAAAT GACATGTGGC TCCCAAAAGC CACCTGGAGC
  721 TGCTCCATCT GTGCCTGGTA GGACAAGTAG CTCTGGAGGT AGGCTGAGTA GGCTGCGTTC
  781 AGTGCCGCCG CCTGCATCCG CTCACCATCC AGGTGTTGGC TCTGTTCCCG CAGGGCTCGG
  841 GTGTCCTGTT CCTTCCTCCA CATGGCCTTT CGGGCCTGAA GCCTGTGGGG GCTTGAAGGT
  901 GTTGGCCACC TTTCCTGGCG AAGATCTGGG GAGTGTTTCG TCCAGCCGCC ACCAGCCCCG
  961 GAGAGCAGGC TGGGCCCGTG TCCGGAGCTT GAGCTGGGGC CGGGGAAGCT CCTCCCAGGG
 1021 GCCAAGAAGG GGGATGACAC AGTCTGGCCG ACCCCTTGTT CGCGTGAAGG GTTGGCTCAT
 1081 TCATGGGCCT TGGTTTCACC CTTGATTGCA GGCTCAGGCG ACCAGTTCCG AAAGTTGGAG
1141 A
//
Primer: pT2KXIG#1 ()
Region: SV40pro/ori, EF-1a pro
Sequence file: RDB16923_A9B7c.seq check
>D06030A1_A9A7_3_pT2KXIG#1_A01_01_ABI24.ab1
    1 CAATAATTCC TCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
   61 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
  121 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
  181 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT
  241 TGCAAAGATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG
  301 TCTTGAAAGG AGTGGGAATT GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA
  361 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC
  421 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG
  481 GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG
  541 CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG
  601 GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC
  661 TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC
  721 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC
  781 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG
  841 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG
  901 GTATTTCGGT TTTTTGGGGG CCGCGGGCGG CGACGGGGGC CCGTGCGTCC CAGCGCACAT
  961 GTTTCGGCGA GGCGGGGCCC TGCGAGCGCG GCCACCCGAG AAATCGGACG GGGGTAGTTC
 1021 TTCAAGCTTG GCCGGGTCTT GCTCTGGATG GCTTGGCCTC GCAGTCGATC GATGTTATCG
1081 ACCCCGACCC TGGGGCCGGA TCAAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oshiumi, H., TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat. Immunol. 4 (2): 161-167 (2003). PMID 12539043. [link to RRC of NBRP]