Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-Rab13

Expression vector of mouse Rab13 fused with EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17260_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert 5' Sequence file: RDB17260_A9Ela.seq
>D06280E6_A9El_2_EGFP-C2_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNCNTGG TCCTGCTGGA GTTCGTGNCC GCCGCCGGGA    61 TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCATGGCC AAAGCCTACG   121 ACCACCTCTT CAAGTTGCTG CTCATCGGGG ACTCGGGGGT GGGCAAGACT TGTCTGATCA   181 TTCGCTTTGC AGAGGACAAC TTCAACAGCA CTTACATCTC TACCATCGGA ATTGATTTCA   241 AGATCCGAAC CGTGGACATA GAGGGGAAGA GGATCAAACT GCAAGTGTGG GACACGGCTG   301 GCCAAGAACG ATTCAAGACA ATAACTACCG CCTATTACCG TGGAGCCATG GGCATTATCC   361 TCGTATATGA CATCACAGAT GAGAAATCCT TCGAGAATAT TCAGAACTGG ATGAAAAGCA   421 TCAAAGAGAA TGCCTCTGCG GGAGTGGAGC GCCTCCTGCT GGGAAACAAG TGTGACATGG   481 AGGCCAAGCG GCAGGTGCAG AGAGAGCAGG CGGAGAAGTT GGCTCGAGAG CACAGAATCC   541 GATTTTTTGA GACGAGTGCC AAATCCAGTG TGAATGTGGA TGAGGCTTTC AGTTCCCTGG   601 CCCGTGACAT CTTGCTCAAG ACAGGAGGCC GGAGATCGGG AACCAACAGT AAGCCCTCAA   661 GCACTGGCCT GAAAACATCT GACAAGAAGA ACAACAAGTG CTTGTTANGC TGAAATCACT   721 AGTGAATTCT GCNGTCGACG GTACCGCGGG CCCGGGATCC ACCGGATCTA GATAACTGAT   781 CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT   841 CNNCNNGANC CTGAANCATN AAATGAATGN AATTGTTGTT GTTNNCTTGT TTATTGCAGC   901 TTATAATGGT TACNAATNAA GCNATAGCAT CNCNAATTTC NCAAANNAAN NCNTTTTTTT   961 CNCNGNATTC NAGTNGNNGN NTTNNNNNAN NTCNTNNNNG NATNNNANCG CNNNANNNNN 1021 NNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17260_A9Elb.seq
>D06280E6_A9El_2_SV40polyA-R2_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNNNNNNNN ATTGCNTTCA TTTTATGTTT    61 CNGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT   121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC   181 TGCAGAATTC ACTAGTGATT TCAGCCTAAC AAGCACTTGT TGTTCTTCTT GTCAGATGTT   241 TTCAGGCCAG TGCTTGAGGG CTTACTGTTG GTTCCCGATC TCCGGCCTCC TGTCTTGAGC   301 AAGATGTCAC GGGCCAGGGA ACTGAAAGCC TCATCCACAT TCACACTGGA TTTGGCACTC   361 GTCTCAAAAA ATCGGATTCT GTGCTCTCGA GCCAACTTCT CCGCCTGCTC TCTCTGCACC   421 TGCCGCTTGG CCTCCATGTC ACACTTGTTT CCCAGCAGGA GGCGCTCCAC TCCCGCAGAG   481 GCATTCTCTT TGATGCTTTT CATCCAGTTC TGAATATTCT CGAAGGATTT CTCATCTGTG   541 ATGTCATATA CGAGGATAAT GCCCATGGCT CCACGGTAAT AGGCGGTAGT TATTGTCTTG   601 AATCGTTCTT GGCCAGCCGT GTCCCACACT TGCAGTTTGA TCCTCTTCCC CTCTATGTCC   661 ACGGTTCGGA TCTTGAAATC AATTCCGATG GTAGAGATGT AAGTGCTGTT GAAGTTGTCC   721 TCTGCAAAGC GAATGATCAG ACAAGTCTTG CCCACCCCCG AGTCCCCGAT GANCANCAAC   781 TTGAAGANGT GGTCGTANGC TTTGGNNNNG GATCTGANTC CNGACTTGTA CAGCTCGNCC   841 ATGCNNAGAN TGNTCCCNGC NGGNNGGTCA CGAACTCNAG CANGANCATG TGATCGCNCT   901 TCTCGTTGGG GGTCTTTGCN CNNGNCNGAN TNGGGTGCTC NNNNANTGGN TTGTCNGGCA   961 GCANCNNNNN GNCCNTCNCC NANNNGGGNG NTCNNCNNGN NANNNNNNNG CNANCNNNNN 1021 NNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB17260_A9Elc.seq
>D06280E6_A9El_2_CMV-Forward_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNT GNNNNNNNTG GGCGGTANGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG   121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA   601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC   781 TGGANTTCGT GACCGCCGCC GGGNTCACTC TCGGCATGGA NNANCTGTAC AAGTNCNGAN   841 TCANATNCAT GGNCNAAGCC TACGANCACC TCTTCAAGTN NCTGCTCATC NGGGANTCNG   901 GGGGNNGGCN ANANTNGTCT GATNNTTCGC TTNNCAGANG NNANCTTNNN NNNCACTTAC 961 NTCTCTANNN NNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB17260_A9Eld.seq
>D06280E6_A9El_2_SV40pro_F_V2_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTN NNNNNNNNCG ATCNAGAGAN AGGANGAGGN    61 NCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG   121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC   181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG   241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC   301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG   361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT   421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG   481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT   541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC   601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG   661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC   721 TATCANGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT   781 GANCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT   841 CGCCTTCTTG ACGANTTCNT CTGNNCGGGA NNCTGGGGGT TCNAAATGAN CGANCAAGCG   901 ACGCCNNANC NNGNCATCAC GANNNTTCNA TTNCACCNCC GNCNTTCTAT GAAANNNNTN   961 GGGCTTCNGN AANNNTTTTT NNNGGNNNNN CNNGNNNGNN GANNCNTNCN NNNNNNGGGN 1021 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl