Resource data sheet
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CSIIR-EF-MCS-IRES2-Venus (#RDB17308)

Lentivirus expression vector, CSII-EF-MCS-IRES2-Venus (RDB04384) with XhoI multi-cloning site.

Clone info. Lentivirus expression vector, CSII-EF-MCS-IRES2-Venus (RDB04384) with XhoI multi-cloning site (MCS).
Comment Expression was confirmed by the deposotor with the observation of Venus expression.
Vector backbone CSII-EF-MCS-IRES2-Venus (RDB04384) (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Amp^r.
Growth conditions LB+Ampicillin, 37oC
Depositor|Developer Yoshitomi, Hiroyuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshitomi H. et al. Nat. Commun. 9(1), 3762, 2018)。リソースの使用は、非営利機関での非営利学術・研究目的に限定する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17308 CSIIR-EF-MCS-IRES2-Venus Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSIIR-EF-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17308).

Reference section:

Yoshitomi, H., Kobayashi, S., Miyagawa-Hayashino, A., Okahata, A., Doi, K., Nishitani, K., Murata, K., Ito, H., Tsuruyama, T., Haga, H., Matsuda, S., Toguchida, J., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]