Short hairpin RNA (shRNA) lentivirus expression vector of human STAT5.
Clone info. | Short hairpin RNA (shRNA) lentivirus expression vector of human signal transducer and activator of transcription 5 (STAT5) shRNA, U6 promoter, GFP. The target sequences for both STAT5A and STAT5B is 5'-GCAGAAACTGTTCAACAACAG-3' with a harpin 5'-CTCGAG-3'. |
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Comment | Expression was confirmed by the deposotor with the observation of GFP expression. |
Vector backbone | CSU6EG (plasmid) |
Size of vector backbone | 10 kb |
Selectable markers | Amp^r. |
Growth conditions | LB+Ampicillin, 37oC |
Gene/insert name | human STAT5A cDNA human STAT5B cDNA |
Depositor|Developer | Yoshitomi, Hiroyuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Ordering instruction.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 付加的使用条件: レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB17322 | CSU6EG_sh2-hSTAT5AB | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The CSU6EG_sh2-hSTAT5AB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17322). |
Reference section:
Kobayashi, S., Watanabe, T., Suzuki, R., Furu, M., Ito, H., Ito, J., Matsuda, S., Yoshitomi, H., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP] |
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