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CSU6EG_sh2-hSTAT5AB (#RDB17322)

Short hairpin RNA (shRNA) lentivirus expression vector of human STAT5.

Clone info. Short hairpin RNA (shRNA) lentivirus expression vector of human signal transducer and activator of transcription 5 (STAT5) shRNA, U6 promoter, GFP. The target sequences for both STAT5A and STAT5B is 5'-GCAGAAACTGTTCAACAACAG-3' with a harpin 5'-CTCGAG-3'.
Comment Expression was confirmed by the deposotor with the observation of GFP expression.
Vector backbone CSU6EG (plasmid)
Size of vector backbone 10 kb
Selectable markers Amp^r.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human STAT5A cDNA human STAT5B cDNA
Depositor|Developer Yoshitomi, Hiroyuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi S. et al. Eur. J. Immunol. 46(2), 360-371, 2016)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17322 CSU6EG_sh2-hSTAT5AB Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSU6EG_sh2-hSTAT5AB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17322).

Reference section:

Kobayashi, S., Watanabe, T., Suzuki, R., Furu, M., Ito, H., Ito, J., Matsuda, S., Yoshitomi, H., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP]