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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF6-FLAG-mouse Ago2 (#RDB17347)

Expression vector of mouse Ago2 tagged with FLAG at N-terminus.

Clone info. Expression vector of mouse argonaute RISC catalytic subunit 2 (Ago2) tagged with FLAG at N-terminus.
Vector backbone pEF6/myc-His (plasmid)
Selectable markers Amp^r (E. coli), BSD (mammalian cells).
Gene/insert name mouse Ago2 cDNA
Depositor|Developer Maruyama, Takashi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17347 pEF6-FLAG-mouse Ago2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF6-FLAG-mouse Ago2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17347).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17347_A9Etp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro, FLAG, insert5'
Sequence file: RDB17347_A9Eta.seq check
>D05962B3_A9Et_1_EF1a-C_F_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNNA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCNTT TCAGGTGTCG TGAGGAATTA GCTTGGTACT AATACGACTC
  121 ACTATAGGGA GACCCAAGCT GGCTAGGTAA GCTTGGTACC GAGCTCGGAT CGCCACCATG
  181 GACTACAAGG ACGACGATGA CAAGGGATCC ACCATGTACT CGGGAGCCGG CCCCGTTCTT
  241 GCTTCTCCTG CTCCGACAAC ATCACCCATC CCAGGATATG CCTTCAAACC TCCACCTCGG
  301 CCGGACTTCG GCACCACCGG GAGAACAATC AAACTACAGG CCAATTTCTT TGAAATGGAC
  361 ATCCCCAAAA TTGACATCTA TCACTATGAA TTGGACATCA AACCTGAGAA ATGCCCTCGG
  421 AGAGTGAACA GGGAAATTGT GGAGCACATG GTCCAGCACT TTAAAACTCA GATCTTCGGG
  481 GACCGGAAGC CAGTGTTTGA TGGAAGGAAG AATCTGTACA CAGCAATGCC CCTTCCGATC
  541 GGCAGGGACA AGGTGGAGCT GGAGGTCACG CTCCCGGGAG AAGGCAAAGA TCGCATCTTT
  601 AAGGTATCCA TCAAGTGGGT GTCGTGCGTG AGCCTGCAGG CGTTACACGA TGCACTTTCG
  661 GGGCGGCTGC CCAGCGTCCC CTTCGAGACG ATCCAGGCCC TGGACGTTGT CATGAGGCAC
  721 TTACCATCCA TGAGGTACAC CCCTGTTGGC CGTTCCTTCT TCACTGCATC TGAANGCTGT
  781 TCCAACCCTC TGGGTGGGGG CAGANAAGTG TGGTTTGGCT TCNATCAGTC CGTCCGANNT
  841 TCTCTTTGGA AAATGATGCT GAATATTGAT GTATCGGCAA CAGCGTTTTA CNNGGCACAG
  901 CCAGNGATCN ANTTTGTTTG TGANNTTTTG NATTTTAAAA GTATTGAAGA NNANNNNNNN
961 NNN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH p(A), insert3'
Sequence file: RDB17347_A9Etb.seq check
>D05962B3_A9Et_1_BGH_rev2_E05_14_ABI24.ab1
    1 NNNNNNNNNG NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACTCAATG GTGATGGTGA TGATGACCGG TACGCGTAGA
  121 ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCTTCG AAGGGCCCTC TAGACTCGAG
  181 CGGCCGCCAC TGTGCTGGAT ATCTGCAGAA TTCTCAAGCA AAGTACATGG TGCGCAGTGT
  241 GTCCTGGTGG ACCTGGACCG CCTTGGCCAG CGCTTGGTGA TCTCGCCCGT TGCTCTGCCC
  301 AGAGGTATGG CTTCCTTCAG CGCTGTCATG TTCTTTATCC ACCAGGTGGT ACCTGGCTCG
  361 GAAGGCCACC AGGTGAGCAT AGTAAGCTGG CGCGGGTATT GACACAGAGC GTGTGCAGCG
  421 CACGTAGGTG TGACACAGCT GGTAGGTCAG AATCTGCAGC TCATCAGAAG AAAAACGATT
  481 GTCATCCCAA AGCACGTGGT AGTGGGAAGG ACGGCTTGTC CCCTGGATGC CGGCGTGACT
  541 GCACAGGTAG AAGTCAAACT CAGTGGGGTG GGTGATCTTC GTGTCCACGG TTGTGCCTGC
  601 GGGAATGTTC CCACTCTTCC CAACCCGCTC ATTTTTGTCT GTGCAGAAGA GGCGTGTGTG
  661 GTGCCGCTTC TGCACCACGA TGAACGTGAT TCCTGGCTGA TAGTCCTTCT CCAGCTTGAT
  721 GCAGGCCTCT CTGATGGCCA GGANCTCATG GTGGANAACC TGCTGGAACT GGCCCTCGGA
  781 GANGCCATCG CGGTANAAGA NGANGCGGGT GGGCTTGAAG CGGGTGGANT TGTAGAACTG
  841 AATGANCAAC TCTCGTACNA TGGCAGCCAG GTCCTGNATG NNCTCCTGTC GGNGCTGCTG
  901 CACGCGCACG GNGNNNCAGN ANCGGTTNGG GNNNGCGTCC NNNCTGNCNN CNANNGCANC
961 AATANNANNN NTNNNNNCNN NNCNNNNCNN NNNGNNGGNN NNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40pro_ori, BSD
Sequence file: RDB17347_A9Etc.seq check
>D05962B3_A9Et_1_SV40pro_F_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGAN GNTTTTTTGG AGGNCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GCCTTTGTCT CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT
  241 CCCCATCTCT GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT
  301 CACTGGTGTC AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG
  361 CACTGCTGCT GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA
  421 CAGGGGCATC TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG
  481 GATCAAAGCC ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT
  541 GCTGCCCTCT GGTTATGTGT GGGAGGGCTA AGCACTTCGT GGCCGAGGAG CAGGACTGAC
  601 ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGNTGGGC TTCGGAATCG
  661 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG
  721 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
  781 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC NAACTCATCA
  841 ATGTATCTTA TCATGTCTGT ATACNGTCNA NNTCTANCTA GANCTTGGCG TAATCATGGN
  901 CATAGCTGTT TCCTGTGTGA AATTGNTATN NGCNNNCAAT TCCNCACNAN NTACNNNNNN
961 GNNNNNTAAA GTGNNANNNN NNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles