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p3XFLAG-CDCA7 WT

Expression vector of human CDCA7 wild-type.

Catalog number RDB17374
Resource name p3XFLAG-CDCA7 WT
Alternative name p3XFLAG-CMV10-CDCA7 WT
Clone info. Expression vector of human cell division cycle associated 7 (CDCA7) wild-type, tagged with 3 x FLAG at N-terminus. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human CDCA7 cDNA
Depositor|Developer Unoki, Motoko |
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External Database
human CDCA7

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
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          RDB17374 p3XFLAG-CDCA7 WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17374_A9Fhp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Region: CMV pro, 3xFLAG, insert 5'
          Sequence file: RDB17374_A9Fha.seq check
          >D06399A1_A9Fh_3_CMV_Forward_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGAATTAAC CATGGACTAC AAAGACCATG
            121 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG CTTGCGGCCG
            181 CGAATTCATC GATAGATCTG ATATCGGTAC CAATGGACGC TCGCCGCGTG CCGCAGAAAG
            241 ATCTCAGAGT AAAGAAGAAC TTAAAGAAAT TCAGATATGT GAAGTTGATT TCCATGGAAA
            301 CCTCGTCATC CTCTGATGAC AGTTGTGACA GCTTTGCTTC TGATAATTTT GCAAACACGA
            361 GGCTGCAGTC AGTTCGGGAA GGCTGTAGGA CCCGCAGCCA GTGCAGGCAC TCTGGACCTC
            421 TCAGGGTGGC GATGAAGTTT CCAGCGCGGA GTACCAGGGG AGCAACCAAC AAAAAAGCAG
            481 AGTCCCGCCA GCCCTCAGAG AATTCTGTGA CTGATTCCAA CTCCGATTCA GAAGATGAAA
            541 GTGGAATGAA TTTTTTGGAG AAAAGGGCTT TAAATATAAA GCAAAACAAA GCAATGCTTG
            601 CAAAACTCAT GTCTGAATTA GAAAGCTTCC CTGGCTCGTT CCGTGGAAGA CATCCCCTCC
            661 CAGGCTCCGA CTCACAATCA AGGAGACCGC GAAGGCGTAC ATTCCCGGGT GTTGCTTCCA
            721 NGAGAAACCC TGAACGGAGA GCTCGTCCTC TTACCANGTC AAGGTCNCGG ATCCTCGGGT
            781 CCNTTGACGC TCTACCCNTG GAGGANGANG ANGAAGAGGA NAAGTACNTG TTGGTGAGAA
            841 AGANGAAGAN NGTGGNTGGN TACNTGAATG AANATGACCT GNCCNNAAGC CNNCGCTCCA
          901 NATCATCCGN NANNNNTTNN NNNTNTNNNT NNNNNNNNNN
          //
          Primer: hGHpolyA-R
          Region: hGHp(A), insert 3'
          Sequence file: RDB17374_A9Fhb.seq check
          >D06399A1_A9Fh_3_hGHpolyA-R_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAGGA NACCTAGTCN GACAAAATGA
             61 TGCAACTTAA TTTTATTAGG ACAAGGCTGG TGGGCACTGG AGTGGCAACT TCCAGGGCCA
            121 GGAGAGGCAC TGGGGAGGGG TCACAGGGAT GCCACCCGGG ATCCTCTAGA TTATGCTTGC
            181 ATTTCAAATT CCTGTTTCAG GCTTTTCAAG TAGGCATGCA CATTCCCAAA GCCATGATAT
            241 TTGGCTAAAT ACACAAGGAC CCCAGTCGCA CACCGTCCAT CTCGCTGCCG GCAGAAACTG
            301 CAGTTGCAGA TTCCTCGACA AGGCGGGCAA TGCCAGTTCG GATCCAGCAG AGCATCCCTG
            361 ACCTCTTCAC CATAACGGTT TCGAAGGCAG GGGCCACAGA ACTGGCCTCG AACGCCCCAG
            421 CAGTCTGGGT TTCTGCAGTT TGTTTTGGTA TCAATAGTCT TCTGACGGCA TTGATGACAA
            481 GTAGAGCCCA GTGAACGGTT ATATATCTTC TCTCGAGAAT TGCTGCAGAC GTTCTCCAAC
            541 TCCTCCTCTG TAATTTCTTC CACTGGGCGA ATTATATGCG GAAGGGTCAC GGATGATCTG
            601 GAGCGACGGC TTCTGGGCAG GTCATCTTCA TTCATGTAGC CNTCCACGGT CTTCCTCTTT
            661 CTCACCAACA TGTACTTATC CTCTTCCTCC TCCTCCTCCA TGGGTAGAGC GTCAAGGGAC
            721 CCGAGGATCC GGGACCTTGA CCTGGNAAGA GGACGAGCTC TCCGTTCAGG GTTTCTCCTG
            781 NAAGCAACAC CCGGNNATGT ACGCCTTCNN GGNCTCNTNG ATTGNGAGTC GGANCCTNNN
            841 ANGGGANGTC TTCCACGGAA CGANCCNGGG AANCTTTCTA ANTNNANANA TGANTTTTGN
            901 NANCATNGCT TTGTTTTGCT TTANATTTAA AGCCCNTTTN NCCAAAAAAT NNATTNNNNT
            961 TTNNTNTTCT GAATNNGANT TGGNATCNNN CACNNNNNNN NCNNANGNNT GNNGGNNNNN
          1021 NGCNTTTTTT NNNNGNNNNN NNNCNNNNNN NNNN
          //
          Primer: SV40pro_F
          Region: NeoR/KanR
          Sequence file: RDB17374_A9Fhc.seq check
          >D06399A1_A9Fh_3_SV40pro_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNGANG NTTTTTTGGA GGANNTGATC
             61 NGCTTGGGAN CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
            121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
            181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
            241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
            301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
            361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
            421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
            481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
            541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
            601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
            661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
            721 NTCGACTGTG GNCGGCTGGG TGTGGCGGAN CNCTATCANG ACATAGCGTT GGCTACCCGN
            781 GATATTGCTG AAGAGCTTGN CGGCGANNGG GCTGANCGCT TCCTCNTGCT TTACNNNATC
            841 NNCGCTCCCN ANTCNNNNNG NATCNNCTTC TATCNNNTNC TTGANNANNN CNTCTGANCG
            901 GGACTCTGGG GGNTCNAAAN TGACCGANNN NNCGACGNNN NNNNCNNNNN NTNNNNNNNN
          961 TNNNANNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl